Overview of selected health surveys in Canada, 1985 to 1991.
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Biomedical subjects
T Ramsay
Publications and source records attributed to T Ramsay.
Adipocyte development in primary rat cell cultures, effect of cell density and serum source.
The development of adipocytes was studied in primary cultures of rat adipose tissue stromal-vascular cells on collagen-coated glass coverslips. The effects of cell density and serum source on lipoprotein lipase (LPL), esterase and lipid histochemistry were evaluated. With a mixture of fetal calf serum (FCS; 2%), horse serum (2%) and pig serum (PS; 10%), large and loosely arranged clusters of adipocytes developed with time through an increase in cell number and size. An inverse relationship was observed between cell size and the number of adipocytes in a cluster. Lower cell densities were associated with large cells and the densest areas contained smaller cells. Unilocular adipocytes were observed by d 13 after plating and were generally absent from the densest area of the coverslips. Histochemically detectable LPL activity was demonstrable before lipid deposition in adipocyte clusters. A comparison of FCS (10%) and PS (10%) as the only serum sources indicated higher level of adipocyte esterase activity and lipid deposition in PS cultures. Cultures of cells from weanling (21 to 28 d old) and old (18 mo) rats were similar, whereas cells from younger rats (2 to 4 d old) formed denser cultures that contained fewer adipocytes. These adipocytes were small (less than 30 micron) and morphologically homogeneous (all multilocular). When cells from the very young rats (2 to 4 d old) were plated at low densities, an inverse relationship between cell size and number of cells in a cluster was also observed.(ABSTRACT TRUNCATED AT 250 WORDS)
Adipose Tissue↗
Adipocyte development.
Our understanding of adipocyte development has advanced rapidly over the last ten years. New developments in adipocyte methodologies have allowed rapid progress. Both histochemical and ultrastructural evaluation of normal and abnormal adipocyte development has increased our understanding of the complexities of cell proliferation and differentiation and the identification of the adipocyte precursor. Adipose tissue transplant studies have shown that some forms of genetic obesity do not involve inherent differences in the adipocyte but do involve some serum factor which predisposes an individual to adipocyte hypertrophy. Cell culture procedures have been developed recently which will allow us to answer more difficult questions concerning adipocyte proliferation and its regulation. The ultimate goals of understanding the complexities of adipocyte development should lead to some practical approaches for the prevention and control of childhood obesity.
Adipose Tissue↗