Studies of a cell line derived from a human malignant melanoma.
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Aged↗
Biomedical subjects
T R Chen
Publications and source records attributed to T R Chen.
Chromosome painting and quantitative karyotyping of colon adenocarcinoma cell lines, DLD-1 and HCT-15.
Chromosome painting by fluorescence in situ hybridization (FISH) was used to examine abnormalities identified by G-banding in colon cancer lines, DLD-1 (ATCC CCL 221) and HCT-15 (CCL 225). DNA libraries from chromosomes comprising these abnormalities (i.e., N2, N8, N11, N16, N17 and N20) were used to prepare paint probes by PCR amplification. Of these paint probes, N2 and N8 exhibited additional chromosome-specific hybridization signals on centromeres that were also useful as a marker for chromosome identification. Results from FISH-painting and G-band analysis were consistent and permitted our quantitative analysis on karyotype evolution in vitro. In DLD-1, predominant cells having trisomic N20 in early passages were replaced by others with disomic N20 in late passages resulting in the trisomic 2p13-23 segment as the only deviation from the diploid content. In HCT-15, predominant cells having t(16;16) and double Y chromosome copies in early passages were replaced by those bearing the paired N16 and single Y chromosome in later passages. Thus cultures changed from the predominant hyperdiploidy to the sole pseudodiploidy with increased number of normal chromosomes.
Adenocarcinoma↗
Coexisting clones in diploid and near-diploid cell lines.
The karyotypic characteristics and clonal composition of human normal diploid MRC-5 (46,XY) and tumorigenic SK-UT-1 (45,XX,-13) and SK-UT-IB (46,XX) cell lines were studied. In MRC-5, 45, XX, -6; 45,X; 46, XY, t(7;14) (p11:q24) and 47, XY, M1 clones were identified. Other karyotypic characteristics included 2.4% polyploidy, 4.8% breaks/gaps, 4.1% structural anomalies, 1.4% hyperdiploidy, and 5.4% hypodiploidy. In SK-UT-1, 45, XX, -13, and clones with t(18;22) (q22.2;q11.23) and 46, XX, 4p+ occurred at 56.6%, 39.3%, and 4.1%, respectively. The t(18;22) karyotypes differed by either paired N13, one N13 plus one i(13q), or one i(13q). The significance of single chromosome amplification relating to the outgrowth in one culture by the t(18;22) clone is discussed. Structural aberrations occurred at high frequencies in N3, N6, and N7, and breaks from these chromosomes were clustered at 3p12, 3q21, 6q21, 6q23, and 7q11.2. The highly malignant SK-UT-IB had the stable karyotype and one 46X, f clone only. Clearly, the cell population was basically mosaic, containing more than one co-existing clone plus the polyploidy of each clone.
Cell Line↗
Liver metastasis of a human colorectal cancer containing two actively growing subclones.
A common pattern of karyotype evolution between clones involves gradual changes of one or only a few chromosomes. Karyotypes of one day-old cultures derived from a tumor nodule of colorectal cancer liver metastasis were studied by GTG chromosome banding. Two karyotypically distinct aneuploid CC-9-a and CC-9-b clones were found respectively, at about 1:3 ratio. Both clones showed common karyotype characteristics with the same number of copies for 13 normal and four marker chromosomes, indicating their common genetic origin. However, CC-9-b differed from CC-9-a by the loss of one copy each of eight normal and four common marker chromosomes. This mode of gross single-chromosome losses is also seen in established cell lines, and probably plays an important role in the drastic chromosome changes associated with the duplication-reduction cycle of karyotype evolution.
Cell Division↗