Biomedical subjects

T Orii

Publications and source records attributed to T Orii.

401 records · Page 23Linked to original sources

PubMedDate not supplied

Immunoglobulin-secreting cells in healthy peripheral blood mononuclear cells.

Immunoglobulin-secreting cells were measured in healthy uncultured peripheral blood mononuclear cells (PBMCs) in vitro. The percentage of IgM-, IgG- and IgA-secreting cells in adult PBMCs was 0.053, 0.099 and 0.065%, respectively. The percentage of IgM-, IgG- and IgA-secreting cells was 0.73, 5.2 and 3.8% of surface IgM-, IgG- and IgA-bearing cells, respectively. The numbers of IgM-, IgG- and IgA-secreting cells increased with age in childhood. However, the numbers of all three classes were slightly decreased in adults compared with children aged 9-15 years. These results may explain the difference in immunity in vivo.

Adolescent↗

PubMedDate not supplied

Evaluation of lymphocyte proliferative responses to food antigens with regard to age and food-specific IgE antibodies in food-sensitive atopic dermatitis.

Peripheral blood mononuclear cells (PBMCs) from patients with atopic dermatitis (AD), selected for sensitivity to hen's egg or cow's milk, showed significant proliferation in response to food antigens, ovalbumin or bovine serum albumin compared with PBMCs from nonatopic control subjects. The proliferative responses of PBMCs to food antigens were evaluated with regard to age and specific IgE antibodies to food antigens in food-sensitive patients with AD. There were no differences in proliferation between each age group, and the proliferation in patients with AD was significantly higher than in age-matched control subjects. There was no relationship between proliferative responses of PBMCs and specific IgE antibodies to food antigens in patients with AD. These results suggest that the responsiveness of lymphocytes to food antigens is independent of age and food-specific IgE antibodies in food-sensitive patients with AD.

Adolescent↗

PubMedDate not supplied

Immunoglobulin-secreting cells in primary immunodeficiency diseases.

To assess immunoglobulin production in vivo, the numbers of immunoglobulin-secreting cells were measured in uncultured peripheral blood mononuclear cells (PBMCs) of patients with primary immunoglobulin deficiency. The numbers of immunoglobulin-secreting cells were found to be variable, and they were well correlated with the serum concentrations of all three major immunoglobulin classes. Our results suggest that measurement of immunoglobulin-secreting cells in PBMCs is useful in the assessment of the ability to produce immunoglobulin in vivo.

Antibody-Producing Cells↗

PubMedDate not supplied

Impaired response to HBcAg in a hepatitis B virus carrier.

Hepatitis B virus (HBV) carriers usually produce antibody to HB core antigen (anti-HBc). We present the case of an HBV carrier who has lacked anti-HBc for a 2-year observation period. Antibody titers against several common viruses (herpes simplex virus-1, varizella-zoster virus and Epstein-Barr virus) were detected in his serum. His lymphocytes proliferated in vitro in response to phytohemagglutinin and concanavalin A. In contrast to other naturally immune or chronic HBV carriers, anti-HBc was not produced by his peripheral mononuclear cells (PBMC) in culture supernatant of pokeweed mitogen-induced anti-HBc production system. These results suggested that he was in a state of impaired specific antibody response to HB core antigen (HBcAg).

Carrier State↗

PubMedDate not supplied

Relationship between interferon-gamma, interleukin-4 and IgE production of lymphocytes from hen's egg-sensitive patients.

We measured in vitro interferon-gamma, interleukin-4 and IgE production of peripheral blood mononuclear cells stimulated with ovalbumin. Interferon-gamma in culture supernatants of ovalbumin-stimulated peripheral blood mononuclear cells from hen's egg- sensitive patients with atopic dermatitis was significantly higher than that of healthy children or hen's egg-sensitive patients with immediate symptoms. Furthermore, in patients with atopic dermatitis who were sensitive to hen's egg and patients with immediate symptoms, there was an inverse relationship between interferon-gamma and IgE production (r = -0.535, p <0.05), and significant correlation was found between interleukin-4 and IgE production (r = 0.802, p <0.05). For the above reasons, IgE synthesis of ovalbumin-stimulated peripheral blood mononuclear cells may be suppressed by interferon-gamma in patients with atopic dermatitis. In contrast, in patients with immediate symptoms, interleukin-4 may play a major role in the pathogenesis of increased IgE production.

Animals↗