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Biomedical subjects

T Oonishi

Publications and source records attributed to T Oonishi.

24 records · Page 2Linked to original sources
PubMed1985

A new standard fluorescence microsphere for quantitative flow cytometry.

A standard fluorescence microsphere has been prepared for quantitative flow cytometry. A uniformly sized (6.53 +/- 0.34 micron in diameter) and perfectly spherical plastic microsphere, Micropearl-FT, was covalently conjugated with fluorescein isothiocyanate (FITC). The characteristic of this microsphere to lyse completely in strong alkaline solutions made it possible to determine the amount of bound FITC easily and reproducibly. The amount of bound FITC depended on the concentration of FITC and the incubation time at pH 9.5 and 37 degrees C. In flow cytometry, the fluorescence intensity of the FITC-conjugated microsphere (FCMS) showed linear dependence on the amount of bound FITC. The fluorescence intensity of FCMS was stable for at least 3 months even in aqueous solutions. Using FCMS, an Epics V was calibrated and the minimum measurable fluorescence intensity was examined. From analysis using this system, the number of FITC-conjugated OKT3 molecules bound to human T lymphocytes was shown to average 4.4 X 10(4) per cell at saturation.

Adult↗
PubMed1983

Mesangiolysis in the glomerular lesion of cyanotic congenital heart disease.

Mesangiolysis associated with glomerulomegaly was observed in an autopsy case of an 11-year-old boy with cyanotic congenital heart disease, i.e. complete transposition of the great vessels with large VSD. Microscopical examination of the kidney revealed marked enlargement of glomeruli with mesangiolysis mainly found in the midzonal cortex involving 16 to 20% of all glomeruli. The glomerular basement membrane in the mesangiolytic lesion showed splitting, between which red blood cells were occasionally encountered. By reconstruction study with serial sectioning, the mesangiolysis was observed to be obviously focal and segmental. Immunofluorescent study revealed no positive results for IgG, IgA, IgM, C3, and fibrinogen/fibrin. By electron microscopic study on the post formalin fixed specimens, no remarkable dense deposits were found in any structural components of the glomeruli. It was assumed that the mesangiolysis might have been provoked by some circulatory disturbances, which superimposed on the preexisting glomerular lesion of cyanotic congenital heart disease in the terminal stage of the clinical course.

Child↗
PubMed1982

Pachymeningitis cervicalis hypertrophica.

Presented here is an autopsy case of pachymeningitis cervicalis hypertrophica. A 77-year-old female had the initial symptoms of cervical radiculopathy which terminated in the syndrome of spinal cord transection. Prior to the onset of radiculopathy, the patient had been admitted to the hospital because of a fever and had been shown to have granulomatous sinusitis. Autopsy revealed chronic granulomatous pachymeningitis of the cervical spinal cord, the lesion being mostly restricted to this region. From histopathologic similarities, the extension of sinusitis to cervical pachymeningitis was assumed, but this was not confirmed. An attempt to discover the causative micro-organisms was unsuccessful. The possibility of a limited form of Wegener's granulomatosis could be excluded in view of the absence of true angiitis. Corresponding to the levels of pachymeningitis, the spinal cord was also severely damaged, which was principally due to circulatory disturbance as a result of pachymeningitis.

Aged↗
PubMed1980

Allergic granulomatosis and angiitis (Churg-Strauss).

An autopsy case of allergic granulomatosis and angiitis of Churg and Strauss is presented. The patient with a history of bronchial asthma of 3 years duration, a mild fever and hypereosinophilia, died in status asthmaticus. Cortocosteroid therapy was not given. The autopsy revealed severe eosinophilic pneumonia, disseminated necrotizing vasculitis of small arteries and veins in various stages, and extravascular granulomatous nodules. The involved organs were the lungs, liver and gastrointestinal tract, but the heart and kidneys were free from these lesions. The differences from the reported cases were discussed.

Adult↗
PubMedDate not supplied

In vivo and in vitro evaluation of the pulsatile mode of a magnetically suspended centrifugal pump.

The authors have been developing a magnetically suspended centrifugal pump (MSCP). They have devised a pulsatile mode for the MSCP, which was generated by altering rotational speed. This article describes in vitro and in vivo studies with the pulsatile mode of the MSCP. Hemolysis tests were performed in two identical circuits to compare the nonpulsatile (NP) mode and the pulsatile (P) mode. In vivo studies were performed in sheep. First, biventricular assisted circulation was instituted in the left heart with the MSCP and in the right heart with the Biopump. The native heart was induced to ventricular fibrillation. Second, a left ventricular assisted circulation was instituted as the native heart was beating. An inflow cannula was inserted into the left atrium in one sheep and into the left ventricle in the other. The normalized indices of hemolysis of the NP and P groups were 0.0025 +/- 0.0018 g/100 L, and 0.0032 +/- 0.0024 g/100 L (N = 4, not significant). During ventricular fibrillation in the P mode, the pulse pressure was 14 mmHg (the rotational speed: 1,500 to 2,600 rpm). In a beating heart, at atrial withdrawal, the pulse pressure increased from 10 to 24 mmHg (2,100 +/- 500 rpm), while at ventricular withdrawal, it decreased from 17 to 40 mmHg (2,000 +/- 500 rpm) on P mode. The MSCP in pulsatile mode did not increase hemolysis. At ventricular withdrawal, it was easier to produce a pulsation than at atrial withdrawal. The pulsatile mode of the MSCP is applicable to a left ventricular assist system.

Animals↗