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Biomedical subjects

T Ono

Publications and source records attributed to T Ono.

At least 523 records · Page 29Linked to original sources
PubMed1996

Expression of perforin in nasal lymphoma. Additional evidence of its natural killer cell derivation.

Eight patients with nasal lymphoma in whom fresh-frozen tissues were available were studied to elucidate the nature of the lymphoma cells. Two cases were diagnosed as diffuse, large cell lymphoma, and the remaining six cases as diffuse, mixed cell types. Immunohistochemical studies revealed that all of the cases were positive for perforin, which is a specific marker for cytotoxic T or natural killer (NK) cells. As all of the cases were CD8 negative, the perforin-positive finding further confirmed the concept that nasal lymphoma is a distinct neoplastic entity derived from NK or NK-related cells. Light microscopic immunohistochemical studies revealed that these nasal lymphoma cases could be classified into Leu19(CD56)+Leu4(CD3)+ (two cases) and Leu19(CD56)+Leu4(CD3)- (six cases) types according to the phenotypes of the proliferating cells. However, simultaneous staining for perforin and Leu4 (CD3) using immunoelectron microscopy on the Leu19+Leu4+ cases showed that the perforin-positive cells were different from the Leu4-positive cells. This finding suggests that the Leu4-positive cells are not neoplastic NK cells but reactive T cells. Six cases were positive for EBER-1 by in situ hybridization analysis. This finding reconfirms the previous studies that Epstein-Barr virus plays a significant role in the pathogenesis of nasal lymphoma.

Adult↗
PubMed1996

Carcinoembryonic antigen and related antigens expressed on keratinocytes in inflammatory dermatoses.

Carcinoembryonic antigen (CEA) is recognized as being important not only as a tumour marker, but also as a cell adhesion molecule in the metastasis of malignant tumour cells, and in the development of inflammatory disorders in some organs. However, the role played by this antigen in inflammatory dermatoses has not been studied previously. To investigate the expression of CEA in inflamed skin, we performed immunohistochemical studies to detect the antigen in both frozen and formalin-fixed, paraffin-embedded biopsy specimens of inflammatory dermatoses, using five different monoclonal antibodies and one polyclonal antibody against human CEA or CEA-related molecules. CEA and CEA-related antigens were expressed by epidermal keratinocytes and by some infiltrating inflammatory cells in inflamed skin. The expression was observed primarily in the upper part of the malpighian layers of the epidermis. On the basis of the distribution of this expression, we suggest an additional role for CEA-related molecules, differing from their roles as tumour markers and adhesion molecules, namely that these molecules may be associated with the state of differentiation of epidermal keratinocytes. Unexpected expression of CEA-related antigens was seen on normal follicular keratinocytes. Thus, it is conceivable that CEA-related antigen may also play a role in the biology of hair follicles.

Antigens, Surface↗
PubMed1996

Clinical characteristics of three patients with UVs syndrome, a photosensitive disorder with defective DNA repair.

Recently, we established a new category of photosensitive disorder termed UVs syndrome. Cells from patients with UVs syndrome have a similar UV sensitivity as xeroderma pigmentosum (XP) cells, but have a normal level of unscheduled DNA synthesis (UDS) unlike XP. UVs syndrome is distinct from Cockayne syndrome (CS) or XP including XP variant (XP-V), as determined by studies of genetic factors using cell fusion, microinjection, and postreplication repair assays. In this study, we identified three Japanese patients with UVs syndrome: an 11-year-old girl, a 17-year-old male, and an 8-year-old boy. The first two patients were siblings, while the third was a case from a different family. All of these patients exhibited acute recurrent sunburn. Common clinical manifestations of these patients were slight erythema and dryness, a number of freckles on sun-exposed areas, and slight telangiectasia only seen on the cheek and nose. Patient 3 showed a lowered minimal erythema dose between 280 and 300 nm. The patients' fibroblasts showed similar characteristics to those in CS, such as UV sensitivity, and a failure of RNA synthesis (RRS) after UV irradiation, despite a normal level of UDS. Thus, UVs syndrome is a new hereditary photosensitive disorder with clinical manifestations similar to a mild form of XP but showing the cellular characteristics of CS.

Adolescent↗
PubMed1996

Excitatory response of rabbit myometrium to nitric oxide in vitro.

Nitric oxide (NO) at high concentration (approx. 33 microM) produced a marked excitation: increase of tension development or increase in amplitude of spontaneous contraction, in 7 out of 8 rabbit nonpregnant myometrial strips. One case produced an inhibition: disappearance of spontaneous contraction. A latent period of several sec usually preceded the excitation. The response of the myometrium to NO approx. 33 microM associated with remarkable increase in tissue cyclic GMP levels. NO approx. 33 microM reduced an inhibition, in 1 out of 3 myometrial strips taken from ovariectomized rabbits. Two cases produced an excitatory. A precursor of NO, L-Arginine 100 microM or an inhibitor of NO synthase, NG-nitro-L-arginine 100 microM also produced a transient weak excitatory response. On the contrary, 8-bromo-cyclic GMP 100 microM produced an inhibition. The excitatory response to NO 33 microM was almost unaffected by pretreatment with indomethacin 10 microM, whereas the spontaneous motility was remarkably depressed. The contractile response of the isolated rabbit myometrium to electrical field stimulation was almost unaffected by the pretreatment with L-arginine 100 microM or NG-nitro-L-arginine 100 microM. The present findings may indicate that NO has inhibitory and excitatory components on the mechanical activity of the rabbit isolated myometrium.

8-Bromo Cyclic Adenosine Monophosphate↗
PubMed1996

[An experimental study on antitumor effect of MMC-fibrin glue mixture].

In spite of postoperative chemo- and/or radiation therapy, the prognosis of advanced cancer patients undergoing palliative operation is still poor. Therefore, in order to improve the effect of chemotherapy, we made a mixture of MMC and fibrin glue (MMC-FIB) as a local chemotherapy. Using FIB with MMC, we expected to enhance the efficiency of MMC by sustained release of MMC. We examined cytotoxic effects and anti-tumor effects of the MMC-FIB using a gastric cancer cell line (MKN-28) and an esophageal cancer cell line (KE-3). Effects of the MMC-FIB were twice as strong as those of MMC on in vitro and in vivo studies. MMC-FIB is easy to make and apply to residual tumor. These results suggest that MMC-FIB is an effective treatment as a local chemotherapy for postoperative residual cancer.

Animals↗
PubMed1996

Enhanced proliferation and IL-1 production of mouse splenocytes by low-dose whole-body X-irradiation.

To elucidate the stimulative effect of low-dose whole-body X-irradiation on the immune system, in vivo, we studied its effects on some immune functions of mouse splenocytes. Results show that Concanavalin A (Con A) and phytohemagglutinin (PHA) responses of splenocytes were significantly increased by irradiation of 2.5 cGy and 5 cGy, whereas lipopolysaccharide (LPS) response was significantly depressed by irradiation of 5 cGy. By irradiation of 2.5 cGy, Con A response was significantly accelerated at each concentration of Con A, but the optimum concentration of Con A shifted to a higher value of 4 micrograms/ml from 2 micrograms/ml in the control group. When blood plasma obtained from 2.5 cGy irradiated mice was added into the medium at 0.005-1%, the Con A response of splenocytes in another un-irradiated mouse was significantly accelerated over that where the plasma added came from the sham-irradiated control mice. Furthermore, 2.5 cGy whole-body irradiation also enhanced the biological activity of intracellular interleukin-1 (IL-1) of LPS-stimulated splenocytes.

Animals↗
PubMed1996

[A case of advanced gastric cancer with multiple liver metastasis successfully treated with combination chemotherapy using UFT, CDDP and etoposide].

We reported a 66-year-old male patient with advanced gastric cancer accompanied by multiple liver metastasis, who responded to combination chemotherapy using UFT, CDDP and etoposide. The patient was administered three courses of 600 mg/body UFT po daily, 50 mg/body CDDP iv and 50 mg/body etoposide iv on days 1 and 8 every 4 weeks. As a result, both the primary and metastatic tumors decreased remarkably in size. Adverse reactions were leukocytopenia (Grade 2), thrombocytopenia (Grade 1) and nausea (Grade 1). he is alive 16 months after the beginning of therapy in a condition of partial response (PR). This combination therapy seemed to be effective for advanced gastric cancer.

Adenocarcinoma↗
PubMed1996

Decreased incidence of thymic lymphoma in AKR mice as a result of chronic, fractionated low-dose total-body X irradiation.

We have investigated whether low-dose total-body X irradiation could suppress the development of lymphoma in AKR mice. Male mice were irradiated with 5 cGy three times a week or 15 cGy two times a week from 11 weeks of age for 40 weeks. The incidences of lymphoma were 80.5% in sham-irradiated mice, 67.5% in mice irradiated with 5 cGy three times a week and 48.6% in mice irradiated with 15 cGy twice a week. Incidence of lymphoma was significantly reduced by irradiation with 15 cGy twice a week (P = 0.006). The mean survival time was significantly prolonged from 283 +/- 3 days in control mice to 316 +/- 10 days in mice irradiated with 15 cGy twice a week (P = 0.008) and to 309 +/- 14 days in mice irradiated with 5 cGy three times a week (P = 0.040). Because about 80% of male AKR mice die of lymphoma, it is likely that the prolonged life span observed in the irradiated mice is due to the reduced incidence of lymphoma.

Animals↗
PubMed1996

Metastatic skin cancer; a case with signet ring cell histology.

We report a 64-year-old Japanese man who developed metastatic skin cancer, in the form of 1-3 cm diameter dome-shaped tumours on his face and head. Histopathological examination demonstrated diastase-resistant periodic acid--Schiff and Alcian blue-positive signet ring cells, suggesting gastric carcinoma. Immunohistochemical staining showed that these cells were positive for carbohydrate antigen CA19-9.

Antigens, Neoplasm↗
PubMed1996

Infantile perianal pyramidal protrusion.

BACKGROUND: The number of reported cases of infantile perianal eruption has been increasing. However, infantile pyramidal protrusion located in the midline anterior to the anus has apparently been reported mainly as acrochordons or skinfolds. OBSERVATIONS: Fifteen infants (14 girls and 1 boy) had a pyramidal protrusion located only in the midline anterior to the anus. Histological examination revealed acanthosis in the epidermis, marked edema in the upper dermis, and mild infiltrates in the dermis. The patients had been brought to the hospital because of swelling of the protrusion. However, all protrusions showed reduction without any treatment. CONCLUSION: Infantile pyramidal protrusion located only in the midline anterior to the anus should be distinguished from acrochordons or skinfolds based on the characteristic clinical features.

Anus Diseases↗
PubMed1996

[Total cavopulmonary connection using a knitted dacron prosthesis impregnated with gelatin (GELSEAL) as an intracardiac conduit].

It is important to find a suitable vascular prosthesis as an intracardiac conduit for a total cavopulmonary connection because of the need for long-term patency. A 7-year-old girl with a double outlet of right ventricle, hypoplastic left ventricle, pulmonary atresia and single atrium with azygos connection underwent a total cavopulmonary connection using a knitted dacron prosthesis impregnated with gelatin (GELSEAL) as an intracardiac conduit. Her postoperative course was uneventful. GELSEAL is soft, easy to hand and effective at preventing blood loss. The conduit is also expected to have long-term patency because of good healing with thin and uniform pseudointimal formation. However, long-term patency especially in the right side of the heart is still unknown. She should be followed with close anticoagulant therapy and careful observation.

Anastomosis, Surgical↗
PubMed1996

Sweat duct milia--immunohistological analysis of structure and three-dimensional reconstruction.

The fine structure of sweat duct milia and the pathomechanism in their aetiology are still unknown. To examine the relationship and connection of milia to the sweat ducts as well as to the overlying epidermis, nine sweat duct milia, six incomplete and three complete, were studied by three-dimensional reconstruction (3DR) analysis based on photomicrographs obtained after histological and immunohistochemical staining with antibodies against carcinoembryonic antigen (CEA), cancer antigen (CA 50) and human cytokeratin 19 (CK 19). In both incomplete and complete milia, an eccrine duct expressing the antigens penetrated into the cyst wall at the centre of its base, formed a circular path within the wall, and opened into the inner cavity. The eccrine duct was mature in eight milia and immature in one. In the cyst wall, CA 50 and CK 19 were detected throughout the entire cyst except for the most apical portion of incomplete milia, where the cyst wall fused with the overlying epidermis which did not express any of the antigens. CEA was distributed mainly in the basal half of the milia. The finding that the path of the eccrine duct within the cyst wall is circular conflicts with the currently accepted concept of simple penetration of the eccrine duct into the wall, suggesting an acrosyringeal origin of the milia. An incomplete milium is the result of fusion between cells derived from an eccrine duct and those derived from the surrounding epidermis, while the formation of a complete milium does not involve this fusion.

Aged↗
PubMed1996

[Preliminary screening for antiviral AIDS drugs. VII. Report for fiscal year 1994].

Preliminary screening of antiviral AIDS drugs has been carried out using three different in vitro assay systems. Among 246 samples of different origin tested, six were shown to inhibit the growth of HIV in vitro. Two of the positive samples have hopeful signs, as the ranges of effective doses are wider than those of most of positive samples which had been found by us.

Anti-HIV Agents↗