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Biomedical subjects

T Onizuka

Publications and source records attributed to T Onizuka.

At least 73 records · Page 4Linked to original sources
PubMed1982

Scar revision.

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Cicatrix↗
PubMed1982

Metabolism of benzo(a)pyrene in epidermal keratinocytes and dermal fibroblasts of humans and mice with reference to variation among species, individuals, and cell types.

The metabolism of benzo(a)pyrene (BP) in epidermal keratinocytes and dermal fibroblasts of humans and mice was investigated with emphasis on variation among species, individuals, and cell types. Human epidermal and dermal cells were isolated from the skin of normal subjects by trypsinization at 4 degrees overnight, followed by separation of the epidermis from the dermis with forceps. In confirmation of previous studies metabolic activity of human epidermal cells on BP was consistently demonstrated by cell-mediated assay, in which V79 Chinese hamster cells were plated on top of sheets of epidermal cells and treated with BP for 48 hr. Mutation of the V79 cells, measured as ouabain resistance, was induced in a dose-related fashion, although the extent of induced mutation varied from 5 to 22 ouabain-resistant colonies per 10(6) survivors/10 microM BP in cultures derived from different individuals. The most striking observation was that human dermal fibroblasts did not activate BP to a form that was mutagenic to cocultured V79 cells. This was observed without exception in all nine cultures of dermal fibroblasts and the one culture of embryo fibroblasts (MR-90) tested. Analysis by high-pressure liquid chromatography indicated that human epidermal and dermal cells both metabolized BP, producing almost the whole series of known metabolites of BP. The amount of BP 7, 8-dihydrodiol, a proximate metabolite of BP, produced by human dermal cells varied from 0.2 to 2.7% of the total BP added and seemed to be enough to induce mutation. Furthermore, human dermal cells not necessarily activated exogenously added BP 7,8-dihydrodiol to a form being mutagenic to V79 cells. These observations suggest that further metabolism of BP 7,8-dihydrodiol is partially or entirely blocked in human fibroblasts. In contrast to human fibroblasts, mouse fibroblasts isolated from the dermis of embryos did activate BP and induced mutation in cocultured V79 cells to a higher extent than did mouse epidermal cells, indicating interspecies variation in metabolic activation of BP between human and mouse fibroblasts.

Adolescent↗
PubMed1980

A new method for the primary repair of unilateral cleft lip.

The author reports primary repair of complete unilateral cleft lip using three flaps: (1) a small triangular flap at the vermilion border; (2) a large triangular flap at the upper lip; and (3) an alar flap. This method has the advantages that the design can be made by any beginner in plastic surgery, reconstruction of the nostril floor is simple, operative results are good and constant even for a beginner; and retardation of the maxilla is minimal or nonexistent.

Cleft Lip↗
PubMed1980

Seven years' survey of our new cleft foot repair.

The reconstruction of lobster-cleft foot is no longer considered from a functional point of view only. The aesthetic appearance of the repaired foot is now considered to be equally important. Since we reported the reconstruction of five toes in 1976, we have operated on 8 patients, totaling 16 cleft foot anomalies. We have followed-up these patients from 3 years to 7 years. As a result, we emphasize that this operative method is excellent in terms of both the function and the aesthetics, and the newly formed toes help to prevent pincer-like deformities. Since those deformities become more serious as a result of weight-bearing, we are convinced that this operation should be done before the patient is 1 year of age, preferably at about 8 months, before walking.

Child, Preschool↗
PubMed1978

A method for repair of cryptotia.

The key points in our method of repair of cryptotia are (1) to cover the skin defect on the posterior aspect of the ear after it is dissected from the head, and (2) to repair the cartilaginous deformity. We describe the use of a temporal skin flap for the former. For the latter, we make parallel incisions on the back of the superior crus and transfer a small graft of conchal cartilage there. The conchal cartilage graft "splints" the repaired crus against cicatricial contracture.

Cartilage↗
PubMed1978

A new method to create a philtrum in secondary cleft lip repairs.

We report an additional surgical method to form a philtrum in cleft lip cases. The key point of this new method is to release the skin tension of the upper lip, thus protecting against the disappearance of the newly-made philtrum. We have obtained better results with this method, in comparison with the other methods previously reported.

Cleft Lip↗