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Biomedical subjects

T Okazaki

Publications and source records attributed to T Okazaki.

At least 559 records · Page 31Linked to original sources
PubMed1969

Mechanism of DNA chain growth. 3. Equal annealing of T4 nascent short DNA chains with the separated complementary strands of the phage DNA.

Nascent short DNA chains isolated from T4-infected E. coli under a variety of conditions anneal equally to the separated complementary phage DNA strands. The samples examined include: pulse-labeled short chains isolated by alkaline sucrose gradient sedimentation from the T4D (wild type)-infected cells in both the early and late stages of phage DNA synthesis; nascent chains accumulated during ligase inhibition of T4 ts B20-infected cells; and the single-stranded nascent short chains isolated from T4D-infected cells by mild procedures involving no denaturation treatment. The results are consistent with the hypothesis that both strands of DNA are synthesized discontinuously.

Carbon Isotopes↗
PubMed1969

Mechanism of DNA chain growth. IV. Direction of synthesis of T4 short DNA chains as revealed by exonucleolytic degradation.

T4 nascent short chains labeled at their growing ends with H(3)-thymidine and uniformly with C(14)-thymidine were prepared, separated into complementary strands, and degraded by E. coli exonuclease I in the 3' to 5' direction or by B. subtilis nuclease in the 5' to 3' direction. The kinetics of release of H(3) and C(14) labels by both enzymes was consistent with the conclusion that the H(3) label is at the 3' end of the nascent short chains of both strands and that the short chains are products of discontinuous synthesis in the 5' to 3' direction along the two template strands.

Bacillus subtilis↗