[Serum 2-5 oligoadenylate synthetase activity in infectious diseases and collagen diseases in childhood].
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2',5'-Oligoadenylate Synthetase↗
Biomedical subjects
T Ohtani
Publications and source records attributed to T Ohtani.
Peptide behaviour and analysis on a chemically stable C18-bonded vinyl alcohol copolymer column with alkaline and acidic eluents.
C18-bonded vinyl alcohol copolymer (ODP) gel showed no weight loss or decrease in column efficiency for alkyl alcohols after being immersed in aqueous solutions of pH 2 and 10 at 50 degrees C for 48 h, and only a 1% weight loss and a slight decrease in alkyl alcohol retention volumes after similar immersion at pH 13. The chemical stability of the ODP gel was further demonstrated in analyses of acidic, neutral and basic peptides on an ODP column with eluents of pH 3-10, which showed that the peptides differ considerably in the sensitivity of their retention behaviour to eluent pH, even though hydrophobic interaction invariably appeared to be the main retention mechanism. The ODP column was therefore applied to the analysis and alignment of lysilendopeptidase (LEP) peptides derived from reduced and S-carboxymethylated carboxyl proteinase (Rcm-P-CP) of Pseudomonas sp. 101. One acid-soluble and seven alkaline-soluble LEP peaks were found in analyses on the ODP column using acidic and alkaline eluents, respectively. The chymotryptic peptides of Rcm-P-CP were first separated on an ODS column with an acidic eluent, and the eight eluates which contained lysine residue, as determined by amino acid analysis, were then analysed on the ODP column with an alkaline eluent, resulting in a further separation of each into several peaks and thus in the recovery of fractions of pure peptides. The LEP peptide alignment was then determined by overlapping the sequences of the chymotryptic peptides with the C- and N-terminal regions of the LEP peptides.
Chromatography↗
A novel CEA-cross-reacting antigen of molecular weight 140,000 expressed on human lymphoid cell lines.
To investigate the possible expression of the carcinoembryonic antigen (CEA) gene family products on lymphoid cells, we screened 28 human cell lines derived from malignant lymphoid cells for reactivity with monoclonal antibodies (MAbs) against CEA and nonspecific cross-reacting antigen (NCA), which is one of the CEA gene family members. Six cell lines (four B cell lines and two non-T, non-B cell lines) were found to react, by a membrane immunofluorescence test, with an MAb, F34-187, which recognizes an antigenic determinant shared between CEA and NCA. None of the 15T cell lines was reactive with any MAbs tested. A glycoprotein antigen isolated with F34-187 from the cell surface showed an apparent molecular mass of ca 140 and 70 kDa in the glycosylated and deglycosylated forms, respectively, and was unreactive with MAbs specific for CEA or NCA, suggesting that the antigen is a new member of the CEA gene family.
Antibodies, Monoclonal↗
Characterization of crystalline L-carnosine Zn(II) complex (Z-103), a novel anti-gastric ulcer agent: tautomeric change of imidazole moiety upon complexation.
A novel crystalline Zn(II) complex of L-carnosine was prepared and evaluated for inhibitory activity against gastric ulceration in rats. The complex was found to be much more active than various other Zn(II) compounds. It was characterized by means of infrared (IR) spectroscopy, solid-state carbon-13 (13C) and nitrogen-15 (15N) 1H-cross-polarization (CP) magic angle spinning (MAS) nuclear magnetic resonance (NMR) spectroscopy and X-ray photoelectron spectroscopy (XPS). The spectroscopic data indicated that L-carnosine coordinates to Zn(II) as a quadridentate ligand. A comparison of the 13C-NMR signals of the imidazole carbons of the complex with those of several histidine derivatives revealed that a tautomeric change of imidazole moiety had occurred upon complexation. The binding mode of the complex was considered to be analogous to that of the corresponding Cu(II) complex.
Animals↗
Studies on serum tocopherol and selenium levels and blood glutathione peroxidase activities in lambs with white muscle disease.
For the investigation of the cause of white muscle disease (WMD), tocopherol (Toc) and selenium (Se) levels and blood glutathione peroxidase (GSH-Px) activities were examined using lambs with WMD and their ewes. Serum Se levels of 4 lambs with WMD were low under 30 ppb, lambs showing very low levels below 15 ppb. The serum Se level was correlated with blood GSH-Px activity showing remarkably low activities in the lambs with WMD. Se contents in the organs of lambs with WMD were lower than those of control lambs, and particularly liver Se contents were deficient levels below 50 ppb. Serum Toc levels were normal, but alpha-Toc contents in organs showed very low levels, especially in the liver. The serum Toc and Se levels and blood GSH-Px activities of their ewes and other sheep kept in the same farm revealed similar results to those of lambs with WMD. Feedstuffs supplied on the farm showed the deficient level of the Se content below 50 ppb and a very low level of alpha-Toc. It was concluded that WMD of lambs in Hokkaido was nutritional muscular dystrophy resulted from deficiencies of Toc and Se to their ewes.
Animals↗
[Six-month intraperitoneal toxicity study of cefpirome sulfate in rats].
A six-month intraperitoneal chronic toxicity study of cefpirome sulfate (CPR) in rats as well as a two-month recovery study were carried out at dose levels of 51.2, 128, 320 and 800 mg/kg/day. The results are as follows. 1. CPR caused no remarkable clinical signs in the general condition of the animals. 2. Body weight gain was depressed in males and females given 800 mg/kg/day. Food consumption, however, was not afected at any dose level. Water consumption increased in males and females at dose of greater than or equal to 320 mg/kg/day. 3. Except an increase in urine volume in males at 800 mg/kg/day, no abnormalities in urinary parameters were found. 4. Frequent decreases in erythrocyte count, hematocrit and hemoglobin concentration were seen in males and females of the 800 mg/kg/day group. At this dose, certain males and females also showed an increase in the reticulocyte count. 5. The serum-biochemical examinations showed a very slight or slight decrease in total cholesterol in males and females given 320 mg/kg/day or more, and increases in uric acid and inorganic phosphorus concentration in some males and females given 800 mg/kg/day. 6. Very slight or slight increases in spleen weight in males and females at a dose of greater than or equal to 320 mg/kg/day, in thyroid weight in females at a dose of greater than or equal to 320 mg/kg/day and in kidney weight in males given 320 mg/kg/day or more as well as in females at 800 mg/kg/day were measured. Further, a decrease in thymus weight was seen in females of the 800 mg/kg/day group. 7. At autopsy, very slight or slight reddish browning of the thyroid was seen in males given 320 mg/kg/day or more and in females given 800 mg/kg/day.(ABSTRACT TRUNCATED AT 400 WORDS)
Animals↗
[Nephrotoxicity of cefpirome sulfate in rabbits--single and multiple intravenous administration].
Nephrotoxic potential of cefpirome sulfate (CPR) was examined by single and multiple intravenous administrations to Japanese white male rabbits. In single administration studies, no nephrotoxic symptoms were observed at the dose level of 320 mg/kg of CPR or less. At the dose level of 500 mg/kg or more, nephrotoxic findings were noted in both CPR and cefazolin sodium(CEZ) groups, such as proteinuria, glucosuria, increase in serum level of urea nitrogen, creatinine and uric acid, and necrosis and calcification in the proximal tubular epithelium of kidney. Renal phenolsulfonphthalein(PSP) excretion was suppressed at the dose level of 500 mg/kg of CPR or more, and 2000 mg/kg of CEZ. In 14 and 21 days repeated administration studies, no nephrotoxic symptoms were observed at the dose level of 100 mg/kg of CPR and CEZ or less. At the dose level of 200 mg/kg of CPR or more, urinary and serum biochemical findings mentioned above were observed, and histopathological changes in the kidney described above were added in 400 mg/kg group. The similar nephrotoxic symptoms including histopathological changes of the kidney were observed in the groups of 100 mg/kg of cefaloridine(CER) and 200 mg/kg of CEZ. In addition, renal PSP excretion was suppressed in the group of 200 mg/kg of CEZ. The results would suggest that CPR is less nephrotoxic than CER and that the nephrotoxic potential of CPR is comparable to CEZ because CPR caused more severe renal failures in single administration study and less severe renal failures in multiple administration study than CEZ.
Animals↗
A case of acinic cell carcinoma of the palate.
A case of acinic cell carcinoma in the palate is reported here. Acinic cell carcinoma is a relatively rare type of adenocarcinoma of salivary gland origin. The patient, an 83-year-old male, complained of swelling in the palate. A red soft mass approximately 8 mm in diameter was noticed in the right maxillary first molar region. Histopathological diagnosis of the surgically removed specimen was acinic cell carcinoma. The tumor had invaded the maxilla. Surgical excision with a border of normal tissue seemed to be an effective form of treatment. The patient exhibited good clinical recovery without recurrence or metastasis.
Aged↗
[Effect of alcohols on nerve growth factor-induced neurite outgrowth from pheochromocytoma PC12h cells].
Effect of alcohols (methanol, ethanol, propanol, butanol, and hexanol) on nerve growth factor (NGF)-induced neurite outgrowth from pheochromocytoma PC12h cells, a subclonal line of PC12, was studied. Although the addition of alcohol alone did not induce neurite development, both the NGF-induced increase in neurite length and the frequency of neurite-bearing cells were enhanced by alcohols. However, this stimulating effect of alcohols was detected only within a short time after NGF and alcohol had been added: differences in the average neurite length and in the frequency of neurite formation between the cells treated with NGF alone and those treated with NGF plus alcohol were not significant at 48 hours and 16 hours, respectively, after the start of treatment. The longer the carbon chain of the alcohol, greater enhancing effect on neurite formation. These results suggest that alcohols did not strengthen the final intensity of the NGF effect to elaborate neurites but rather shortened the time required for process formation in response to NGF by presumably increasing the fluidity of plasma membranes of PC12h cells.
Alcohols↗
[Clinical application of T-Scan System. 2. Evaluation of soft mouth guards].
Soft mouth guards are effective to prevent traumatic injury in the maxillofacial region. In this study, we tried to find out the effect of soft mouth guards on the distribution and the magnitude of occlusal force using the T-Scan system. Ten adult subjects volunteered to exert maximum biting force wearing three different types of soft mouth guards. With the limitation of the T-scan system reported in our previous article, following results were obtained. 1) Occlusal forces were more evenly distributed with soft mouth guards than without them. 2) Softer mouth guards were more effective for occlusal force distribution. 3) Since occlusal relationship between mouth guards and mandibular arch could alter the distribution pattern a great deal, occlusion of mouth guards should be examined more carefully.
Adult↗
[Mouth guard and occlusal force distribution].
Mouth guards are effective to prevent traumatic injury in maxillofacial region during sports activities. This study was conducted to investigate the influence of wearing mouth guards on occlusal force distribution with the T-scan system. With comparison to the value of average occlusal force level per occlusal point (OFL) on natural dentition and wearing conventional full arch bite plane, following results were obtained. 1) Difference of OFL within the same dental arch became smaller when mouth guards were on. 2) Statistically significant difference in OFL was not found between two mouth guards with different hardness. 3) During three seconds clenching, OFL in posterior region changed great deal at the initiation but stayed at about the same level after that.
Bite Force↗
[Combination chemotherapy with etoposide, ADM, and CDDP (EAP) for advanced gastric cancer].
EAP therapy has been performed on 50 cases of advanced gastric cancer from January 1988 to September 1989. Adriamycin 20 mg/m2, Cisplatin 40 mg/m2 and Etoposide 100 mg/m2 were administered on day 1 and 7, 2 and 8, and 4, 5 and 6, respectively, with not less than 2 courses every 3 to 4 weeks. Complete success, PR, NC and PD were obtained in 48, 21, 20 and 7 cases, respectively, the rate of effectiveness being 43.8% with a confidence interval 95% of 30-58%. The rate of effectiveness by lesions for evaluation was high (30.4, 100, and 50% for primary lesion, Virchow's lymphnodal metastasis and liver metastasis, respectively). MST was 5.1 months for EAP therapy, which was highly effective but led to no prolonged survival period. Thus, it is thought that good control of leukopenia, a dose limiting factor remains to be examined.
Adult↗
[A 38-year-old survivor with complete transposition of the great arteries].
A 38-year-old woman with complete transposition of the great arteries (CTGA) was reported. She was admitted to Ehime University Hospital because of dyspnea on effort. She was diagnosed as CTGA with large ventricular septal defect (VSD) and pulmonary stenosis (PS) by magnetic resonance imaging. Survivors above 30 years of age are very rare in CTGA. The reason why this patient is still alive at the age of 38 seems to be due to PS that decreases pulmonary vascular resistance and VSD that mixes blood gas appropriately in the ventricle.
Adult↗
[Clinical application of T-scan System. 1. Sensitivity and reproducibility and its application].
Analyzing occlusal contacts is significant for establishing the occlusal scheme as well as for the diagnosis on dysfunction of stomatognathic system. We tested a new occlusal contact analyzing device (T-Scan: Tekscan Inc. Boston, U.S.A.) for its sensitivity and reproducibility in relation to its clinical application. Following results were obtained. 1) As the modeling of dental arch of this system was not always correct, much care should be needed for identifying the contact location. 2) The minimum amount of load needed for displaying each five step occlusal force increased linear fashion (step 1: 250 g and step 5: 1750 g) when the sensor was vertically pressed on the intersection of force sensitive line. The minimum amount of force changed with the loading condition such as area and direction. Distortion of the sensor could lead the measurement error. 3) Since the time measurement data by this system were shorter than actual length of time and were measured over about 250 g load, light and early occlusal contacts could not be detected.
Dental Arch↗
[Two cases of intra-arterial infusion of antibiotics in intractable chronic osteomyelitis of the mandible].
Two cases of intractable chronic osteomyelitis of the mandible were encountered recently. The first of these was a 51-year-old man who complained of swelling in the right cheek and the mandibular region. The other was a 27-year-old man who chiefly complained of swelling, pain and trisms in the right mandible. Both patients were treated with antibiotics and the osteosclerotic tissue was removed. The antibiotics were injected into the superficial temporal artery using a one-shot technique. Radiographs of the patients taken one to two years after the treatment revealed normal conditions with no signs of recurrence. It appears that this form of treatment is effective in the case of intractable chronic osteomyelitis of the mandible.
Adult↗
[Diagnosis of the infiltrating depth of the colonic cancer by endoscopic ultrasonography (EUS)].
Endoscopic ultrasonography (EUS) is the latest procedure in the field of gastroenterology and is highly evaulating now because of its unique diagnosing method. For the example, the intra- and extra-mural informations of the GI tract can be known through this technique. In this paper, we reported on the usefulness of EUS in the diagnosis of the depth invasion of the colonic cancer and introduced the new and exclusive instrument for the colon. Namely, we could obtain the accurate diagnosis in 18 out of 20 cases with the rectal or the sigmoid colon cancer. One of causes of misdiagnosis in 2 cases of early cancer was due to the ghost echo. Another cause was the cancer cells infiltration to the deeper tissue which could be recognized only by a microscope. Then, we tried to examine the entire colon in 2 cases using the new instrument and succeeded to insert the endoscope to the cecum in a short time. In conclusion, we were convinced that EUS would develop the new aspect in the diagnosis of the colonic diseases including cancer.
Colonic Neoplasms↗
Open dorsal metacarpophalangeal dislocations of the index, long, and ring fingers.
An open dorsal dislocation of the metacarpophalangeal joints of the index, long, and ring fingers was treated satisfactorily by early reduction.
Adult↗
Hematoporphyrin derivative uptake by atheroma in atherosclerotic rabbits: the spectra of fluorescence from hematoporphyrin derivative demonstrated by an excimer dye laser.
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Animals↗