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Biomedical subjects

T Ohtake

Publications and source records attributed to T Ohtake.

At least 127 records · Page 7Linked to original sources
PubMed1990

[Pindolol-induced rhabdomyolysis in sarcoid myopathy].

A 72-year-old man suddenly developed severe muscle weakness following the treatment with pindolol (Calvisken) for three days. Neurological examination on admission disclosed marked proximal muscle weakness with absent deep tendon reflexes. Laboratory data showed significant increase of serum CK, aldolase and myoglobin. Electromyography revealed both neurogenic and myogenic changes in all muscle tested. Skeletal muscle CT showed patchy low density areas in muscles of thigh and leg, especially in the hamstrings. Femoral muscle biopsy demonstrated a granulomatous nodule with multinucleated giant cells in the degenerated muscle fibers showing small-grouped atrophy. By Mb-PAP staining, Mb-negative fibers were randomly distributed among normally stained ones. Leu 1, 3a, 4, HLA-DR positive cells were found adjacent to the granuloma by immunoperoxidase staining. After immediate withdrawal of pindolol and treatment with steroid, he recovered muscle strength and enzyme activities were normalized in a week. Beta blockers have been known to induce muscle cramps or pain and moderate elevation of serum muscle enzymes. However, severe muscle weakness with highly elevated enzyme activities leading to rhabdomyolysis as noticed in the present case was rarely reported in the literature. Underlying sarcoid myopathy might be suspected to exaggerate this unusual case of pindolol-induced rhabdomyolysis. A careful use of pindolol is emphasized.

Aged↗
PubMed1989

Posterior fossa subdural empyema in the term neonate.

A case of posterior fossa subdural empyema following neonatal meningitis is reported. Computerized tomography (CT) carried out on the 11th day after birth showed an obstructive hydrocephalus with diffuse cerebellar swelling. Ventricular drainage was carried out to control the hydrocephalus and antibiotics were given intravenously. A second CT scan taken on the 21st day after birth revealed a loculated subdural empyema in the retrocerebellar space. Serial CT scans showed a diminution in the size of the empyema.

Anti-Bacterial Agents↗
PubMed1989

Fundamental study on latex reagents for agglutination tests.

Competitive adsorption of Fab and Fc fragments on to particles revealed that the main driving forces for the adsorption of Fab and Fc fragments are ionic and hydrophobic forces, respectively. Latex particles were sensitized with antihuman C-reactive protein-antibody under a condition where ionic binding force was suppressed, and hence antibody was supposed to attach to the particles predominantly at the Fc site. The resulting latex indicated a high efficiency for the determination of C-reactive protein. Among the latexes used, a partially hydrolysed styrene-acrylamide copolymer latex was the best with respect to test efficiency and storage stability.

Adsorption↗
PubMed1989

Efferent connections of the nucleus reuniens and the rhomboid nucleus in the rat: an anterograde PHA-L tracing study.

Direct projections from the nucleus reuniens (Re) and the rhomboid nucleus (Rh) in the midline of the thalamus were examined in the rat by utilizing anterograde axonal transport of Phaseolus vulgaris leukoagglutinin (PHA-L). The Re projected axon terminals densely to the anterior cingulate cortex, infralimbic area of the medial frontal cortex, agranular insular cortex, entorhinal cortex, and parasubiculum. Labeled terminals in these cerebral cortices were distributed mainly in layer I, except for the entorhinal cortex where labeled terminals were distributed in layers I-IV. On the other hand, labeled terminals of projection fibers from the Rh to the cerebral cortex were distributed in layers II-VI of the anterior cingulate cortex, frontal cortex, parietal cortex, and agranular insular cortex. In the nucleus accumbens, caudate putamen, and basolateral amygdaloid nucleus, labeled terminals from the Rh were more dense than those from the Re. In the hypothalamus, labeled terminals from the Re were more dense than those from the Rh. The septal nuclei and field CA1 of Ammon's horn received labeled axon terminals from the rostral portion of the Re, but not from the caudal portion of the Re or the Rh. The patterns of distribution of axon terminals from the Re and Rh were similar in other subcortical target regions. To confirm the PHA-L results, wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP) was injected into two projection sites of PHA-L-labeled axons. After injection into the agranular insular cortex, WGA-HRP-labeled neurons were found in the Re, but only a few were present in the Rh. In contrast to this, after WGA-HRP injection into the nucleus accumbens, WGA-HRP-labeled neurons were found in the Rh, but only a few were seen in the Re.

Animals↗
PubMed1989

Amyloid urinary-tract calculi in patients on chronic dialysis.

Urinary calculi found in 4 patients on chronic hemodialysis or continuous ambulatory peritoneal dialysis (CAPD) were identified as protein calculi by infrared spectroscopic analysis. Positive Congo red staining and immunological assessment revealed that the calculi were composed of amyloid protein derived from beta 2-microglobulin. A comparison of the patients who excreted calculi with 10 patients on chronic dialysis without urinary calculi showed no significant differences in the urinary and serum levels of beta 2-microglobulin. The mechanism of amyloid calculus formation may involve factors independent of the concentration of beta 2-microglobulin in urine or serum. Urinary calculi found in patients on chronic hemodialysis or CAPD were composed of amyloid protein derived from beta 2-microglobulin.

Amyloid↗
PubMed1989

[Cardiac response during various activities in patients with ischemic heart disease evaluated by an ambulatory ventricular monitor (VEST)].

To assess the cardiac response to various exercise in ischemic heart disease (IHD), left ventricular function was continuously measured with an ambulatory ventricular monitor (VEST) in 3 normal subjects and 15 IHD patients. Treadmill exercise (early stage (E1), end stage (E2) and recovery standing state (RE], walking (WK) and climbing stairs (CS), was used for exercise. 15 IHD patients were divided into 4 groups by the LVEF response to treadmill exercise. In group 1 (G1), LVEF increased gradually at stage 1 and became plateau from stage 2 to end stage like normal group. In group 2 (G2), LVEF increased at stage 1 and became plateau from stage 2 but decreased from maximal EF more than 5% at end stage. In group 3 (G3), LVEF increased only at stage 1 and decreased immediately after stage 1 to end stage. The decrease of LVEF at end stage from standing was more than 5%. In group 4 (G4), LVEF decreased at stage 1 and became minimum at end stage. This grouping was well related to thallium redistribution (Tl RD) score and coronary arteriography (CAG) score. We could predict the severity of coronary artery disease from this grouping because all group 3 or 4 patients had severe coronary artery disease. VEST was useful for the evaluation for the tolerance to daily activities. Group 1, 2 and 3 tolerated daily activities while group 4 could not always. The degree of the increase in cardiac output during ischemia evaluated by VEST may be one of the useful index of the tolerance to exercise. In conclusion VEST was very useful for evaluating cardiac capacity of ischemic heart disease patients during various exercise.

Coronary Disease↗
PubMed1989

[A case of spinocerebellar degeneration with bilateral MLF syndrome and dystonia].

The patient, a 31-year-old married woman, noticed spasticity on walking at the age of 19 accompanied by ataxia, dysarthria and dysphagia. Facial twitching and dystonic movement of extremities have been observed since age 27. A sister of her father showed the similar ataxia and dysarthria, and expired of pneumonia at the age of 45. On admission at the age of 29, neurological examinations revealed nystagmus, marked spasticity with pathological reflexes and clonus, cerebellar ataxia, dysarthria and dysphagia, diffuse muscle wasting, fasciculation in facial musculature, and generalized slow dystonic movement. By neuro-otological studies bilateral MLF syndrome with upward gaze limitation and decreased velocity of saccadic eye movement were detected. Surface EMG at rest showed a dystonic discharges on the extremities. Needle EMG disclosed a systemic neurogenic change with reduced interference and high amplitude potentials. Atrophy of the brainstem was remarkable on the cranial CT and MRI. These abnormal eye movements, especially bilateral MLF syndrome and generalized dystonia seem to be quite unusual in the variety of spinocerebellar degenerations. On reviewing detected clinical descriptions on Joseph disease this case can be probably included.

Adult↗
PubMed1989

[N-isopropyl I-123 p-iodoamphetamine (IMP) brain SPECT in Alzheimer's disease].

Eighteen patients with Alzheimer's disease (AD), 5 patients with Pick disease (PD), 6 patients with other types of degenerative dementia (O) and 12 age-matched normal control subjects (N) were studied using N-isopropyl p-[I-123]iodoamphetamine (I-123 IMP) with SPECT. Regional to cerebellar activity (R/CE) ratio and frontal to parietal (F/P) activity ratio were evaluated in each case. I-123 IMP-SPECT revealed focal abnormality in all cases in AD, PD, O group, while XCT and/or MRI were normal or showed cerebral atrophy without focal abnormal density or intensity. In AD group, R/CE ratio in all the regions except for bilateral Rolandic area and left primary visual cortex were significantly lower (p less than 0.05 or p less than 0.01) than that in N group, and F/P ratio were significantly higher (p less than 0.01) than that in P and O group. In conclusion, I-123 IMP-SPECT is useful to detect focal perfusion abnormality in dementia and may be of value in differentiating Alzheimer's disease from dementia of non-Alzheimer type.

Aged↗
PubMed1988

ST-T isointegral analysis of exercise stress body surface mapping for identifying ischemic areas in patients with angina pectoris.

ST-T isointegral analysis of body surface mapping was used in an attempt to localize ischemic areas on exercise tests. In 28 patients with angina pectoris and 10 healthy subjects, body surface potential was recorded with 87 leads, and ST isopotential and ST-T isointegral maps were constructed. In all 10 healthy subjects, the basic pattern of the ST-T isointegral map showed no significant change after exercise. In 23 of 28 patients with angina pectoris (82%), alterations in the ST-T isointegral map after exercise were observed. They were divided into four types (anterior, inferoposterior, lateral, and global) according to the distribution of negative values, which were well correlated with the extent of ischemic area determined by thallium myocardial scintigraphy and coronary angiography. The postexercise ST-T isointegral map was normalized after administration of nitroglycerin in four of five patients. In five patients (18%) who did not show abnormalities on the postexercise ST-T isointegral map, the magnitude of maximal ST depression was significantly smaller than that observed in the other 23 patients with angina pectoris (0.14 vs 0.23 mV on the average, p less than 0.05). It was concluded that the exercise test with ST-T isointegral mapping is a new method for noninvasive detection of location and severity of ischemic regions.

Adult↗
PubMed1988

Experimental study of vascularized bone: quantitative analysis of bone scintigraphy and histology.

The purpose of this study was the sequential evaluation of vascularized bone by quantitative analysis of bone scintigraphy with Tc-99m methylene diphosphonate (MDP). The correlation between the uptake of Tc-99m MDP and the morphologic behavior of vascularized bone was investigated, simultaneously with bone labeling, contact microradiogram (CMR), and histology of an undecalcified sample. In this experiment, 41 adult female rabbits were used. An island tibia pedicled with the popliteal vessels was used as the model of vascularized bone. The maximum uptake of Tc-99m MDP was observed at two to four postoperative weeks. When bone marrow was partially exposed, the uptake was significantly large. On the basis of this experimental study, the degree of Tc-99m MDP uptake seemed to depend primarily on the osteoblastic activity of vascularized bone. The newly-formed bone was observed mainly at periosteal sites.

Animals↗
PubMed1988

[The effect of water rinsing in removal of endotoxin from exposed root].

Previous studies have revealed that periodontally involved exposed roots show evidence of biologic toxicity possibly due to endotoxin from subgingival micro-organisms. Eleven periodontally involved teeth and five periodontally healthy teeth comprised the material. The teeth were rinsed in ultrasonic cleaner containing pyrogen-free water for one hour. The samples were taken from the solution after 1, 5, or 60 minutes. With the aim of removing residual endotoxin on the roots not eliminated by rinsing, each tooth was subjected to extraction of endotoxin with 45% phenol in water for 90 minutes at 65 degrees C. After extraction of endotoxin, the root surface of the involved teeth was further planned with a hand scaler. Endotoxin was extracted from the particles removed from the root surfaces. All samples were tested for endotoxin by limulus amoebocyte lysate assay. The amount of endotoxin from involved teeth was 4,500 +/- 2,000 ng per tooth and from healthy teeth it was 370 +/- 120 ng per tooth. The amount of residual endotoxin on roots from involved teeth was 32 +/- 20 ng per tooth and from healthy teeth it was 1.2 +/- 1.1 ng per tooth. The amount of endotoxin collected from particles removed from previously rinsed involved root surfaces was 1.5 ng per tooth. The rate of removal of endotoxin by water rinsing of a tooth was 64 +/- 25% in 1 minute, 85 +/- 16% in 5 minutes, 99 +/- 0.6% in 60 minutes.

Endotoxins↗