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Biomedical subjects

T Ohno

Publications and source records attributed to T Ohno.

At least 685 records · Page 38Linked to original sources
PubMed1988

Effect of noradrenaline on plasma hormones and metabolites in cold-acclimated rats.

The effects of noradrenaline (NA) injection (40 micrograms/100 g, i.p.) on plasma glucagon, corticosterone (CS), deoxycorticosterone (DOCS), glucose, and free fatty acids (FFA) were investigated in cold-acclimated rats (CA) and warm controls (WC). The CA were transferred to warm control temperature ca. 18 h prior to NA or saline injection. The animals were decapitated and the trunk blood was collected 0, 10, 20, and 40 min after the injection. Plasma glucagon level increased significantly during the experimental period after NA, while saline did not influence its level. The magnitude of increase as assessed by 95% confidence interval was significantly greater in CA than in WC. Plasma CS and DOCS were significantly increased by saline injection, but NA caused greater elevations of these steroid-hormones. The magnitudes of increases were also greater in CA. In contrast to the hormonal responses, NA-induced increases of plasma glucose and FFA levels were smaller in CA than in WC. These results suggest that glucagon and glucocorticoids are released, at least partly, by NA, and these thermogenic factors act in synergism, enhancing nonshivering thermogenesis through an accelerated utilization of energy substrates such as glucose and FFA.

Acclimatization↗
PubMed1988

Gangliosides and energy substrates in brown adipose tissue of cold-acclimated rat.

Effect of cold acclimation on gangliosides as well as triglyceride and glycogen in brown adipose tissue was studied in rats. Ganglioside GM3 level per unit fresh weight and per unit fat-free-dry-matter was significantly higher in cold-acclimated rats (CA) than in warm controls, while the tissue triglyceride and glycogen levels were lower in CA. GM3 may be involved in cold-induced proliferation and differentiation of brown adipose tissue.

Acclimatization↗
PubMed1988

Incidence and ultrastructure of rudimentary cilia in benign and malignant peripheral nerve tumors.

The incidence and ultrastructure of cilia were examined in 26 peripheral nerve tumors. The cilia had the common features of elongated shafts and basal structures consisting of two basal bodies. The basal body contained two basal feet and some long striated rootlets. The cilia lacked central tubules and two dynein arms of subfiber A, suggesting that they were nonmotile and rudimentary. The quantitative results showed that the incidence of cilia was low in Schwann cells and fibroblasts of the 20 benign tumors and slightly higher in perineurial cells of the 12 neurofibromas. Conversely, the incidence was high in most of the 6 malignant tumors. Only one perineurial cell from normal mature nerve examined carried a cilium, although no cilia were seen in the other perineurial cells, Schwann cells, and fibroblasts observed. The results showed that ciliary formation was more marked in the more actively proliferating tissues such as malignant tumors, and less so in the less actively proliferating tissues such as benign tumors. Ciliary formation may be one of the ultrastructural features commonly observed in proliferative tissues.

Cilia↗
PubMed1988

Alkaline bismuth solution as an en bloc stain for formaldehyde-glutaraldehyde potassium permanganate fixed fungal spores.

An alkaline solution of bismuth subnitrate reacted well with the cell membranes and cell walls of formaldehyde-glutaraldehyde potassium permanganate fixed Alternaria spores, demonstrating them with greater contrast than in sections stained with uranyl acetate and lead citrate. Optimal fine structure of fungal spores was obtained by en bloc staining with alkaline bismuth solution after aldehyde and permanganate fixation. The contrast of the cell organelles and cell walls was high enough in sections cut after the alkaline bismuth en bloc stain for direct ultrastructural observation. Our results indicate that the alkaline bismuth stain is useful either as an en bloc or section stain for aldehyde and permanganate fixed fungal spores.

Alternaria↗
PubMed1988

Anterolateral uncoforaminotomy for cervical spondylotic myeloradiculopathy.

Thirty-five patients with cervical spondylotic radiculopathy and myeloradiculopathy had anterolateral uncoforaminotomy. Spondylotic spurs associated with radiculopathy were continuous from the uncovertebral joint to the posterior ridge of the vertebral body in 33 patients and to the posteriorly bulging disc with posterolateral bony spurs in 2 patients. Anterolateral uncoforaminotomy was found safe to remove the continuous type of spur, resulting in decompression of the cord-root complex, which shifted anteriorly after surgery. The outcome was satisfactory in 19 of 20 radiculopathy patients, and in all 15 myeloradiculopathy patients. Decompression of the cord-root complex is the most important factor in relieving neurologic manifestations of cervical spondylosis.

Adult↗
PubMed1988

Clinical response to busramustine (KM-2210) in chronic lymphocytic leukemia: a pilot evaluation of estrogen receptor in relation to its therapeutic effect.

Busramustine (KM-2210), the benzoate of a 17 beta-estradiol-chlorambucil conjugate, was administered to 11 patients with chronic lymphocytic leukemia (CLL) which included eight cases of B-cell CLL and three cases of T-cell CLL. Four patients had received prior chemotherapy. Busramustine was given orally at an initial daily dose of 50-100 mg continuously, and the dose was modified according to hematological improvement. Two cases of B-cell CLL achieved clinical complete responses, six cases including two of T-cell CLL and four of B-cell CLL achieved partial responses and one case of B-cell CLL achieved improvement. The partial and complete response rate was 72.7%. Four patients showed estrogen receptor activity of CLL cells ranging from 3.5 to 57.5 fmol/mg cytosol protein, but there seemed to be no correlation between the estrogen receptor activity of the CLL cells and the therapeutic effects of busramustine. Toxic effects included diarrhea (2/11) and estrogen-related symptoms including breast pain (4/11), genital bleeding (2/5), gynecomastia (2/6) and loss of libido (2/6). The findings of this preliminary study suggest that busramustine is effective in the treatment of CLL, irrespective of the presence of the estrogen receptor.

Adult↗
PubMed1987

Simple method for the analysis of food dyes on reversed-phase thin-layer plates.

A technique for the determination of food dyes using reversed-phase thin-layer chromatography on octadecyl-modified silica is described. A solvent system containing 5.0% aqueous sodium sulphate solution enables good separation of the food dyes. Their separation is dependent on the pH of the solvent system, good separation among all dyes being obtained between pH 6.0 and 7.0. The determination of thirteen dyes can be achieved by a combination of methanol-acetonitrile-5.0% aqueous sodium sulphate solution (3:3:10) (solvent system A) and methanol-methyl ethyl ketone-5.0% aqueous sodium sulphate solution (1:1:1) (solvent system B). Solvent system A was used for the determination of Tartrazine, Amaranth, Indigo Carmine, New Coccine, Sunset Yellow FCF, Allura Red AC, Fast Green FCF and Brilliant Blue FCF and for the screening of Acid Red (R-106), Eosin (R-103), Erythrosin (R-3), Rose Bengale (R-105) and Phloxine (R-104). When the spots of R-3, R-103, R-104, R-105 and R-106 appear on the plate, their determination can be achieved by using solvent system B. Each calibration graph was linear between 0.2 and 1.0 micrograms.

Chromatography, Thin Layer↗
PubMed1987

Irritative and protective activity of mild irritants in rat stomach.

Exposure of the stomach for 30 min to acidified sodium taurocholate (TC) (1-20 mM) or sodium salicylate (SA) (10-80 mM) caused a reduction of transmucosal PD and an increase of luminal pH in anesthetized rats, in a concentration-related manner. Acidified aspirin (ASA) (10-80 mM) reduced PD in the same manner, without significant effect on pH. Histologically, these agents similarly produced damage to the surface cells. After a 30-min exposure to either 20 mM TC or 40 mM SA, acid secretion ceased and bicarbonate (0.5-1 mumol/10 min) appeared in the lumen, whereas acid secretion persisted in the stomach exposed to 40 mM ASA. However, under cimetidine infusion (8 mg/kg/hr) these agents produced similar degrees of luminal alkalinization (approximately 1 mumol/10 min). Pretreatment with indomethacin (5 mg/kg, subcutaneously) significantly inhibited the increase of pH seen after exposure to 20 mM TC, but had no effect on the increase of pH caused by 40 mM SA. Concurrent administration of 16,16-dmPGE2 (3 micrograms/kg, subcutaneously) significantly antagonized the effect of indomethacin in the stomach exposed to 20 mM TC and even increased the pH in the stomach exposed to 40 mM ASA. After a 3-hr exposure to these agents, there was macroscopically apparent damage only in the stomach exposed to ASA, although the PD was similarly reduced in response to either agent. The levels of PGE2 in the corpus mucosa were significantly increased in stomachs exposed to 20 mM TC and 40 mM SA, but decreased in those exposed to 40 mM ASA.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1987

Granular cell tumor of the ascending colon: a case report.

A granular cell tumor (granular cell myoblastoma) of the ascending colon in a 48-year old male is reported. The tumor was detected by barium enema study as a sessile polyp, and colonofiberscopy revealed submucosal tumor. It was removed by endoscopic polypectomy. Macroscopic examination showed the characteristic features of granular cell tumor. The avidin-biotin-peroxidase complex (ABC) method for detection of S-100 protein demonstrated that the cytoplasm of tumor cells and the pleomorphic nuclei were strongly stained with anti-S-100 protein serum, which supports the concept of the Schwann cell origin of granular cell tumor.

Colonic Neoplasms↗
PubMed1987

Selective deficiency in IL-2 production and refractoriness to extrinsic IL-2 in immunodeficiency with hyper-IgM.

Various lymphokines are inducible by the stimulation of T-cell mitogens, phytohemagglutinin, and concanavalin A. A 32-year-old female with an atypical type of immunodeficiency with hyper-IgM was evaluated for possible defects in the production of several immunoregulatory lymphokines. Although the mitogens appeared to bind effectively to the specific surface receptors of patient peripheral blood lymphocyte (PBL), the proliferative responses were significantly decreased. The culture supernatant of patient PBL stimulated by the mitogens contained only a trace amount of interleukin 2 (IL-2) activity. Addition of recombinant IL-2 to the cultures concomitantly with the mitogens could not restore the decreased responses of patient PBL. Tac antigen expression of patient PBL induced by the mitogens was moderately impaired. These data suggest that there is a defect in both IL-2-producing and IL-2-responding cells. In contrast, the culture supernatant of mitogen-stimulated patient PBL contained B-cell growth and differentiation factors as well as interferon-gamma activities equal to those of the control. These results suggest that there are independent regulatory pathways for the production of IL-2 and other T-cell-derived lymphokines.

Adult↗
PubMed1987

Regional differences in the cat caudate nucleus as to the effectiveness in inducing contraversive head-turning by electrical stimulation.

An attempt was made to re-examine regional differences in the cat caudate nucleus as to the effectiveness in inducing contraversive head-turning by electrical stimulation and to analyze the time course of head-turning quantitatively. In 5 of the total 9 cats, the right sensorimotor cortex and its surrounding areas had been ablated chronically. While the awake, unrestrained cat maintained a stable standing posture facing forward, stimulation was applied systematically to various points in and around the caudate nucleus with a movable stimulating electrode. Trains of stimulating current pulses of less than 300 microA were given, mostly at a rate of 100 Hz for 5 s. In most experiments in which stimulation was given to the side of the intact cerebral cortex, stimulation of caudal portions of the head of the caudate nucleus was effective in inducing contraversive head-turning, but that of its rostral portions was ineffective. In experiments on the side of chronic cortical ablation, similar results were obtained. These results suggested that head-turning induced by stimulation of the caudate nucleus was brought about not by the activation of the corticofugal fibers from these cortical areas by a current spreading to the internal capsule, but by the activation of caudate neurons. Hence, it was demonstrated that there were regional differences in the cat caudate nucleus as to the effectiveness in inducing head-turning. The mean of the shortest latencies of the onset of head-turning for individual stimulation points was 396 ms (S.D., 210 ms) for the side of the intact cerebral cortex, and 454 ms (S.D., 289 ms) for the side of the cortical ablation. Statistically, there was no significant difference between them. Therefore, it was further revealed that the elimination of the sensorimotor cortex did not affect the caudate-induced head-turning in terms of the latency of its onset.

Animals↗
PubMed1987

Nucleolus-associated J chains in myeloma cells: clinical significance.

Bone marrow aspirates from 20 patients with multiple myeloma (MM), 4 with smoldering multiple myeloma (S-MM), 1 with idiopathic Bence Jones proteinuria (I-BJP), and 6 with primary macroglobulinemia (PMG) were examined for nucleolus-associated J chain. The incidence of nucleolar J chain-positive (J+) cells among nucleolated cells producing M-component was measured. This incidence (94.0-100%) in terminal MM was significantly higher than that (0-58.0%) in non-terminal MM. Judging from a low incidence in the remission phase, chemotherapy might cause a selective elimination of less differentiated myeloma cells with J+ nucleoli and might have some effect on J chain synthesis. The incidence of nucleolar J+ cells was very low in S-MM. The IgM cells in PMG, where J chain is present in a disulfide-linked form, had no or few J+ nucleoli. No correlation between the incidence of nucleolar J+ cells among nucleolated plasma cells and the percentage of nucleolated cells or that of J+ cells was found. Large J+ nucleoli seemed to be another morphological feature indicating anaplastic myeloma cells. A high incidence of nucleolar J+ cells may be one of the indicators for progressive disease.

Biomarkers, Tumor↗