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Biomedical subjects

T Oda

Publications and source records attributed to T Oda.

At least 919 records · Page 51Linked to original sources
PubMed1976

Metabolism of maltose during surgery in patients with diabetes mellitus under general anesthesia.

During a surgical operation under general anesthesia, maltose was administered at a rate of 1g/kg BW to 9 diabetic and 18 non-diabetic patients. The disappearance curve of maltose from blood was similar between diabetics and non-diabetics. Blood sugar tended to rise but no significant difference was found between the two groups. Lactic acid and pyruvic acid showed a tendency toward a rise, but the change was non-significant. NEFA and uric acid failed to change. Urinary excretion of maltose was not different between diabetics and non-diabetics. After 120 min., urinary excretion of maltose was similar between the two groups, but excretion as glucose was greater in the diabetic group.

Adult↗
PubMed1976

Molecular sieving by glomerular basement membrane isolated from normal and nephrotic rabbits.

Analog chromatography with glomerular basement membrane isolated from normal and nephrotic rabbits was performed to see if there was a molecular sieving effect by the basement membrane. In a normal glomerular basement membrane column, 125I-labeled human serum globulin and 131I-labeled human serum albumin were eluted at the same position, probably at the void volume of the column. However, 125I-labeled insulin and tritiated water appeared with a delay and gave separated peaks. In nephrotic glomerular basement membrane, 125I-labeled human globulin, 131I-labeled serum albumin, 125I-labeled insulin and tritiated water gave four distinctly separated peaks in that order. By analogy with Sephadex, the sieving effect of glomerular basement membrane was demonstrated.

Animals↗
PubMed1976

Increased urinary excretion of L-xylulose in patients with liver cirrhosis.

The urinary excretions of L-xylulose, xylitol and D-glucarate after the oral administration of glucuronolactone (5 g) were measured in normal healthy persons, patients with diabetes mellitus, acute hepatitis in recovery stage, chronic hepatitis and liver cirrhosis. In normal subjects, the mean value of L-xylulose excretion was 14.6 +/- 1.4 mumol/2 h with a range from 6.5 to 21.8. Marked increase of L-xylulose excretion was observed in cirrhotic patients, the mean value was 97.1 +/- 19.8 with a range from 22.0 to 236.6. Though some cases of acute and chronic hepatitis showed higher values than the normal range, no case exceeded 50 mumol/2 h. The urinary excretion of xylitol in cirrhotic patients was also higher than normal no increase was observed in D-glucarate excretion. The values of L-xylulose excretion in cirrhosis were correlated with the values of serum total bilirubin, albumin, albumin/globulin ratio, lactate dehydrogenase and prothrombin time. These findings indicate that the measurement of L-xylulose in urine after the oral glucuronolactone loading provides a useful tool for evaluation of the severity of liver cirrhosis.

Acute Disease↗
PubMed1976

Characteristics of a human cell line successively transformed by Rous sarcoma virus and simian virus 40.

Human enbryo cells were successively transformed by the Schmidt-Ruppin strain of Rous sarcoma virus (SR-RSV) and simian virus 40 (SV40) in vitro, and the double transformant HuE 13 RS was established. From this cell line the two clonal cell lines RSa and RSb were isolated. In both, presence of SV40 T antigens was demonstrated by the fluorescent antibody technique, and the presence of RSV genomes was verified in one RSb clone by focus formation after fusion with chick embryo cells. Growth of these cells was affected by dibutyryl cAMP without marked morphologic changes. Cells were extremely sensitive to the anticellular action of human leukocyte interferon.

Antigens, Viral↗
PubMed1976

Effects of mannoheptulose and DL-glyceraldehyde on glucose induced insulin release and adenosine 3',5'-monophosphate levels in isoalted islets of rat pancreas.

The effects of mannoheptulose and DL-glyceraldehyde on glucose-induced insulin release and cycli AMP levels in islets isolated from rat pancreas were investigated. Mannoheptulose inhibition on glucose-induced insulin release was observed after only 5-min incubation period, indicating an inhibitory effect on the early phase of insulin release. This inhibition on insulin release was accompanied with the simultaneous depression of cyclic AMP levels in islets. By the addition of DL-glyceraldehyde to the medium in which glucose and mannoheptulose were present, the depressed cyclic AMP levels in islets were recovered to the control level completely but the restoration of insulin release in the early phase was not complete. In the absence of glucose, DL-glyceraldehyde did not demonstrate a significant increase of insulin release during 5 min incubation, though a marked stimulation was observed after 30-min incubation. Cyclic AMP levels in islets were not affected by DL-glyceraldehyde. When DL-glyceraldehyde was added to the medium with glucose, significant inhibition of glucos-induced insulin release in its early phase was observed without the reduction of cyclic AMP levels in islets. From these findings, the following possibilities are suggested and discussed. 1. Maintenance of the cyclic AMP levels in islets is a necessary but insufficient condition for glucose-induced insulin release particularly for its early phase. 2. Glucose-induced insulin release seems to depend on both the binding of glucose with glucoreceptor and the supply of some metabolites. Mannoheptulose inhibits both mechanisms. DL-glyceraldehyde may supply metabolites but competitively inhibit the binding of glucose to the glucoreceptor.

Animals↗
PubMed1976

The presence of phosphate-binding protein in inner mitochondrial membrane.

Phosphate-binding protein(s) was found in the inner mitochondrial membrane of calf heart by Sephadex G-200 and G-25 gel filtration. The binding activity was inhibited by N-ethylmaleimide and competed by a large amount of cold phosphate. The amount of phosphate bound to the fraction was 29 nmoles per mg of protein. Affinity chromatography with phosphate-bound Sepharose 4B confirmed the presence of phosphate-binding protein(s) in the active fraction of mitochondrial membrane fractionated by gel filtration.

Carrier Proteins↗
PubMed1976

Changes in the activities of nucleoside triphosphatases and nucleoside kinases in Vero cells infected with Simian virus 40.

Changes in the activities of three enzymes involved in nucleotide metabolism were studied following infection of Vero cells with SV40. The results showed that SV40 infection enhanced the activities of uridine phosphokinase and UMP phosphokinase involved in nucleotide synthesis and suppressed the activity of nucleoside triphosphatases involved in nulceotide degradation. These SV40-induced enzyme activity changes probably served to somehow increase the quantity of nucleotides for virus proliferation.

Animals↗
PubMed1976

Cellular metabolic control by chemical modification of cell membrane.

Various reagents used in the chemical modification of amino- and carboxy-groups of proteins, and of carbohydrates of glycoproteins and glycolipids, inhibit respiration in ascites tumor cells concomitant with release of potassium ion from those cells. The respiratory activity of washed ascites tumor cells is increased by exogenous addition of potassium ion. The lowered respiratory control index as well as oxidative phosphorylation of aged mitochondria are restored upon increasing the potassium concentration of the incubation mixture in the presence of respiratory substrates. The data suggest that the potassium ion level of cells is changed by modifying physicochemical properties of membrane components and that cellular energy metabolism is regulated by intracellular potassium ion concentration.

4-Aminobenzoic Acid↗
PubMed1976

[Gonotrophic dissociation in Culex pipiens pipiens L. (author's transl)].

Under certain conditions gonotrophic dissociation has been observed in Culex pipiens pipiens. To contribute to a better understanding of the factors which govern the appearance of this phenomenon and of its ecological importance a population in Northern Germany has been investigated. Follicular development, feeding activity and occurrence of gonotrophic dissociation were examined in females obtained from wild-caught larval stages and kept under outdoor conditions after emergence in July and August. In addition overwintering females collected in cellars in September and October were investigated after reactivation by exposure to 16 hours illumination periods and 28 degrees C. In females emerging in August the follicles were generally small, the feeding activity was very low but the rate of gonotrophic dissociation appeared to be higher than that in mosquitoes which had emerged in July. Overwintering females had small follicles and did not take a blood meal during the first days under reactivation conditions. Only after five days a development of follicles and an increase in feeding activity became apparent. Through the effect of long illumination and high temperature the state of diapause was gradually overcome and the females were fully reactivated. At the same time the rate of gonotrophic dissociation in fed females which was high in the early time of exposure to reactivation conditions became low. This suggests that gonotrophic dissociation occurs commonly in females with low feeding activity, i.e. those which are not reactivated completely. In Northern Germany the overwintering females are unfed and nulliparous and gonotrophic dissociation does not seem to play an important role for the overwintering of the mosquito in this area.

Animals↗