Search PubMed⌕ Search

Biomedical subjects

T Noma

Publications and source records attributed to T Noma.

At least 127 records · Page 7Linked to original sources
PubMed1989

Allergen-specific induction of interleukin-2 (IL-2) responsiveness in lymphocytes from children with asthma. I. Antigen specificity and initial events of the induction.

Interleukin-2 (IL-2) responsiveness of Dermatophagoides farinae (Df)-stimulated lymphocytes from children with bronchial asthma was studied. Six-day culture of lymphocytes from allergic patients increased after an additional 3 days of incubation with recombinant IL-2. This phenomenon was not observed when the lymphocytes of patients allergic to Df were stimulated with ovalbumin (OVA). Normal lymphocytes stimulated with Df expressed Tac antigen (low-affinity IL-2 receptor) but, in contrast to the patients' lymphocytes, did not absorb nor respond to IL-2. Nonadherent responder cells cultured with Df-pulsed autologous adherent cells acquired IL-2 responsiveness, but those cultured with OVA-pulsed adherent cells did not. The monoclonal antibody to HLA-DQ framework (Leu 10 and clonab DQ), but not to HLA-DR framework (OKIa1) and HLA-DP (HLA-DP and clonab DP-DR), blocked the antigen-presenting cells from inducing IL-2 responsiveness. Nonadherent responder cells depleted of OKT4 (CD4)-positive cells failed to acquire IL-2 responsiveness, whereas depletion of OKT8 (CD8) cells had no impact. Taken as a whole, the results indicate that DQ-bearing adherent cells from allergic donors play a key role in presenting Df antigen to allergen-specific responder T cells, which are very likely to be members of the OKT4 positive subset.

Absorption↗
PubMed1989

Allergen-specific induction of interleukin-2 (IL-2) responsiveness in lymphocytes from children with asthma. II. Regulation of IL-2 responsiveness by supernatants of normal lymphocytes.

Induction of interleukin-2 (IL-2) responsiveness by Dermatophagoides farinae (Df)-stimulated lymphocytes from children with asthma was markedly suppressed after the addition of culture supernatants from Df-stimulated normal T cells. Ovalbumin (OVA)-induced IL-2 responsiveness of lymphocytes from patients with hen-egg allergy was not suppressed by the supernatant from Df-stimulated T cells, indicating the antigenic specificity of the effect. Patients' lymphocytes whose IL-2 responsiveness was decreased still expressed Tac antigen (low-affinity IL-2 receptors) but, in contrast to the patients' original lymphocytes, did not absorb or respond to IL-2, suggesting the loss of high-affinity IL-2 receptors (p55/p75) from these cells. The supernatant from Df-stimulated normal T cells from one individual did not necessarily suppress the response of all patients tested, indicating the existence of some allogeneic barrier between the factor(s) and patients' lymphocytes.

Absorption↗
PubMed1989

Effect of human recombinant interleukin 4 on in vitro granulopoiesis of human bone marrow cells.

Utilizing in vitro colony assay, we investigated the effect of human recombinant interleukin 4 (hIL-4) on granulopoiesis of normal human bone marrow cells. Though hIL-4 itself did not possess any colony-stimulating activity, the number of neutrophil (N) colonies, particularly the number of small colonies, supported by human recombinant G-CSF (hG-CSF) was significantly increased when hIL-4 was used as a costimulant. In contrast, the number of eosinophil (Eo) colonies supported by hIL-5 was decreased when hIL-4 was used as a costimulant. Also, the numbers of N and Eo colonies supported by hIL-3 or hGM-CSF were both significantly decreased when hIL-4 was added. These data suggest that hIL-4 has diverse positive and negative regulatory effects on human neutro- and eosinophilopoiesis as a cofactor of various CSFs.

Adult↗
PubMed1989

[Anodontia. 4: Crown shape of teeth of the anodontia with anhydrotic and hypohydrotic ectodermal dysplasia].

The purpose of this study was to investigate the morphological variation of teeth of the partial anadontia with anhydrotic and hypohydrotic ectodermal dysplasia. Qualitative and quantitative observations were made for the upper central incisor and upper first molar in the anadontia of 18 patients. Moreover, some phylogenetic consideration was taken on the variability of the shapes. The results were as follows. 1) The crown shapes of 28 upper permanent central incisors were classified into 5 types based on the presence and/or size of mamelons, from rudimental teeth such as the cone-shaped type to the almost normal but flat type. Also these morphological variation had a clear correlation to the number of unabsenced teeth of same side. 2) The relatively reduced mesio-distal size, which was said to be a strong trait which shows a tendency towards degeneration in human upper permanent molars, was not observed in the upper 1st permanent molar of the anadontia. 3) Of the 33 1st permanent molars, contraction of the hypocone and contraction of the metacone were independently found in 4 and 10 teeth respectively. 4) Protoconule, which are rudimental small cusps and mesial to the protocone, was observed in all 33 molars, and there was a correlation between the extent of their development and the number of remaining teeth on the same side. The relative length between the top of the paracone and protocone, also, correlated to the number of the remaining teeth of same side. 5) Morphological variations of the upper central permanent incisors and upper 1st molars of the anadontia reflected the phylogenetic process in human teeth to some extent. It seemed that the less was the number of remaining teeth of same side, the earlier phase it suggested in morphogenesis of human teeth.

Anodontia↗
PubMed1989

[Study on the effect of disodium cromoglycate (DSCG) on antigen induced IL-2 responsiveness].

We tested the effects of disodium cromoglycate (DSCG) on antigen-induced IL-2 responsiveness in lymphocytes from patients with atopic dermatitis and/or bronchial asthma. Patient lymphocytes pretreated with 5 x 10(3) micrograms/ml DSCG for 24 or 48 hours failed to induce the responsiveness to IL-2 on stimulation with Dermatophagoides farinae (Df) or ovalbumin (OVA) antigen. DSCG-treated adherent cells were blocked to present the antigen to nonadherent responding cells for induction of IL-2 responsiveness. In contrast, DSCG-treated non-adherent cells, recombined with antigen-activated adherent cells, acquired IL-2 responsiveness. However, Con A-activated lymphocytes from the same patients were not affected by the same treatment. The results indicate that DSCG is capable of suppressing antigen-induced IL-2 responsiveness but not the response induced by mitogen such as Con A.

Antigens↗
PubMed1989

[In vitro detection of ovalbumin-specific IgA producing cells from lymphocytes of patients with hen-egg allergy].

We studied antigen-specific IgA production from the peripheral lymphocytes of children with hen-egg allergy by using the method of indirect plaque forming cell (PFC) assay. The number of OVA-specific IgA-PFC generated from the patient lymphocytes was low (6 +/- 3/7 X 10(4) non T cells) in comparison with age-matched normal children (112 +/- 18/7 X 10(4) non T cells). The numbers of OVA-specific IgG-PFC and IgM-PFC generated from the patient lymphocytes were not so different from those of the age-matched normal children. This indicates that the activity of OVA-specific IgA-antibody production was reduced in patients with hen-egg allergy.

Child, Preschool↗
PubMed1989

[Thyroglobulin (Tg)-specific induction of IL2 responsiveness in lymphocytes from patients with Hashimoto thyroiditis and its dependency on HLA-DQ antigen].

The peripheral blood mononuclear cells from Hashimoto thyroiditis patients acquired IL2 responsiveness in response to thyroglobulin stimulation, when they were partially depleted of adherent cells. Non-adherent cells reconstituted with Tg-pulsed autologous adherent cells at the ratio of 9:1 also acquired IL2 responsiveness, but those cultured with OVA (ovalbumin)-pulse adherent cells did not. This indicates Tg-induced IL2 responsiveness was specific for the antigen. Although there was no clear correlation between the intensity of induced IL2 responsiveness and the serum titer of anti-Tg antibody, Tg-induced IL2 responsiveness was significant in the seropositive patients. Pretreatment of the mononuclear cells with the monoclonal antibody to the HLA-DQ framework (Leu 10), but not the HLA-DR framework (OKIal), blocked the induction of IL2 responsiveness. This indicated that DQ-bearing adherent cells play a key role in presenting Tg antigen to the responder cells.

Adult↗
PubMed1989

Demonstration of clonal proliferation of T lymphocytes in early neoplastic disease. Studies with probes for the beta-chain of the T cell receptor and human T cell lymphotropic virus type I.

This study demonstrates that the detection of rearrangement of the T-cell receptor gene and adult T cell leukemia virus or human T cell lymphotropic virus type I (ATLV/HTLV-I) integration in the genome are sensitive and practical methods for the diagnosis and characterization of cutaneous T cell neoplasms. Biopsy specimens obtained from small skin nodules containing a dense infiltration of lymphocytes were analyzed by Southern blotting with the use of probes for both the beta-chain of the T-cell receptor gene and the ATLV/HTLV-I genome. DNA samples from one patient with early, chronic adult T cell lymphoma or leukemia, revealed the monoclonal proliferation of lymphocytes. For another patient with early cutaneous T cell lymphoma analysis by Southern blotting with the beta-chain probe of DNA from three separate lesions revealed identical rearranged bands, indicating not only the monoclonal proliferation of T cells in each lesion but also the clonal origin of each lesion. In contrast, analysis by Southern blotting of DNA samples extracted from three nodules from a patient with lymphomatoid papulosis showed no rearranged band and therefore no clonal proliferation. DNA extracted from blood lymphocytes of these patients failed to hybridize with the probes described above as a rearranged band. These results indicate that analysis of DNA extracted from skin lesions may have diagnostic value in determining monoclonal proliferation of lymphocytes in patients with early stage cutaneous lymphomas.

Adult↗
PubMed1988

Both B151-T cell replacing factor 1 and IL-5 regulate Ig secretion and IL-2 receptor expression on a cloned B lymphoma line.

In the present study, we have demonstrated that both B151-T cell-replacing factor 1 and rIL-5 are responsible for the activity to partially induce CL-3 cells into IgM-synthesizing cells and also to synergize with IL-2 to augment IL-2R expression on and IgM synthesis in CL-3 cells. These actions of rIL-5 on a homogeneous cloned line (BCL1-CL-3 cells) allow us to identify and characterize the two alternated B cell developmental pathways. One is an IL-2-independent, IL-5-driven differentiation pathway without preceding up-regulated IL-2R expression, and the other is an IL-5 plus IL-2-dependent augmented differentiation pathway with preceding up-regulated IL-2R expression. We have also demonstrated the functional difference of two distinct B cell growth-promoting factors, B cell-stimulating factor 1 (rIL-4) and rIL-5. CL-3 cells are equally stimulated to grow by rIL-4 and rIL-5, whereas only rIL-5 can render CL-3 cells responsive to rIL-2, indicating that these two lymphokines affect B cells in a strikingly different manner.

Animals↗
PubMed1988

Interferon gamma modulates the ability of autologous non-T cells to stimulate T cells to produce and respond to interleukin 2.

The interactions of T-cell receptor with self-Ia antigen on non-T cells induced IL-2 production and IL-2 receptors on the cell surface and thus responsiveness to IL-2 of T cells in autologous mixed-lymphocyte reaction (AMLR). Four-day-cultured autologous non-T cells lost their ability to stimulate T cells to produce and respond to IL-2 with concurrent decrease of HLA-DR and HLA-DQ antigen expressed on the cell surface. Culturing of non-T cells with 500 U/ml of recombinant interferon gamma (IFN-gamma) maintained their stimulating ability which was otherwise lost. Treatment of non-T cells with monoclonal anti-HLA-DR or anti-HLA-DQ antibody before mixture with T cells abrogated their ability to induce IL-2 production and IL-2 responsiveness of T cells. The combined data suggested that Ia antigen expressed on non-T cells is modulated by IFN-gamma, which increases the ability of non-T cells to stimulate autologous T cells to produce and respond to IL-2.

Cell Communication↗
PubMed1988

Effect of human recombinant interleukin 5 and G-CSF on eosinophil colony formation.

Human recombinant (r) IL-5 was shown to have the activity to stimulate eosinophil (Eo) colony formation from human non-T, non-adherent bone marrow cells. The majority of these colonies were found to contain a small number of basophils, macrophages or neutrophils. Human rG-CSF, which alone did not stimulate Eo colony formation, showed an enhancing effect on Eo colony formation when added with IL-5. IL-5 seems to stimulate the proliferation and differentiation of CFU-Eo, while G-CSF acts on the early stage of eosinophilopoiesis.

Cell Differentiation↗
PubMed1988

Application of a human T cell line derived from a Sézary syndrome patient for human interleukin 4 assay.

Interleukin 4 (IL-4) is a multi-functional biological regulator molecule. We developed a simple assay system for human IL-4, using a IL-2-dependent T cell line designated Sez 627 cell line. Sez 627 cells were found to respond only to human IL-4 and human IL-2, but not to other available lymphokines. In combination with mouse IL-2-dependent CTLL-2 cell line, which responds to both human and murine IL-2 but not to human IL-4, human IL-4 activity can be discriminated from human IL-2 activity.

Biological Assay↗
PubMed1988

Molecular cloning and structure of the mouse interleukin-5 gene.

We isolated the chromosomal gene for mouse interleukin-5 (IL-5) from genomic libraries using a cloned mouse IL-5 cDNA as probe. Nucleotide sequence determination of the IL-5 gene and its flanking regions showed that the gene was composed of four exons. The sequence homologies of the exons 1, 2, 3, and 4 of the human and murine IL-5 genes are 70, 79, 85, and 77%, respectively. TATA-like and CAAT-like sequences were found 25 and 52 base-pairs, respectively, upstream of the transcription initiation site which was identified by S1 mapping analysis. The nucleotide sequences of the mouse and human IL-5 genes were about 70% homologous in the 5'-flanking region extending to 200 base-pairs upstream of the transcription initiation site, beyond which the homology declined rapidly. The sequence surrounding 50 base-pairs upstream of the initiation site was conserved among genes for other lymphokines: such as mouse granulocyte/macrophage colony stimulating factor, mouse IL-2, and mouse IL-4.

Amino Acid Sequence↗
PubMed1988

Down-regulation of suppressor cell induction.

A unidirectional cascade of cell interactions has been described previously that involves at least three distinct populations of suppressor T cells (Ts) that interact in appropriate succession to mediate suppression of delayed hypersensitivity responses to the 4-hydroxy-3-nitrophenylacetyl hapten (NP). The present work focuses on the potential bidirectional effects of one suppressor factor and how it can regulate the homeostatic mechanisms that maintain this suppressor cell cascade. Specifically, the effects of prior administration of mice with a transducer suppressor factor (TsF2) on the generation of NP-specific TS1 suppressor cells were evaluated. It was observed that the TSF2 given 2-14 days prior to administration of the tolerogen (NP-coupled splenic-adherent cells) interfered with the development of inducer TS1 suppressor cells. This down-regulation of suppressor cell induction is mediated by a population of cells that have the following characteristics: NP-binding, Lyt-1+2-, L3T4+, I-J+, and Vicia villosa (VV)-adherent T cells (for convenience these cells are termed anti-suppressor cells). Furthermore, there are genetic restrictions (both H-2 and IgH) between TSF2 and host which control the generation of anti-suppressor cells. The results suggest that TSF2 may have a role in homeostatic mechanisms that regulate the NP-specific suppressor cell cascade. The relationship of anti-suppressor and contra-suppressor cells is discussed.

Animals↗
PubMed1987

Molecular cloning and structure of the human interleukin-5 gene.

We isolated the chromosomal gene for human interleukin-5 (IL-5) from human genomic libraries using a cloned human IL-5 cDNA as probe. Nucleotide sequence determination of the IL-5 gene and its flanking regions showed that the gene consisted of four exons and three introns. TATA-like and CAAT-like sequences reside 26 and 73 base pairs, respectively, upstream of the transcription initiation site identified by S1 mapping analysis. The 5'-flanking region of the IL-5 gene has sequences homologous with those of the corresponding regions of the genes for human granulocyte/macrophage colony-stimulating factor, murine IL-4, human interferon-gamma, and human IL-2. The intron-exon organization and location of the cysteine residue suggest that the IL-5 gene is phylogenetically related to the genes for the granulocyte/macrophage colony-stimulating factor, IL-4, and IL-2, although their amino acid sequences are not significantly conserved.

Amino Acid Sequence↗