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Biomedical subjects

T Noguchi

Publications and source records attributed to T Noguchi.

At least 631 records · Page 35Linked to original sources
PubMed1991

Utilization of trans-activated T4 uvsY regulatory signal for a high yield of cloned gene products.

We established an efficient system for a high-level production of foreign gene products in Escherichia coli using a trans-activated gene expression under the control of a phage T4 regulatory signal cloned in a plasmid by T4 phage infection. The transcriptional and translational signal of the T4 uvsY gene cloned in a plasmid was fused translationally with the coding region of the lacZ gene. When E. coli cells carrying the uvsY-lacZ plasmid were infected with cytosine-substituting T4 phage at a multiplicity of infection of 5, the amount of beta-galactosidase increased about 2-fold (trans-activation) over that without phage infection. We examined conditions for the high-level production of a trans-activated gene product. We found that a large number of T4-infected cells in a lysis-inhibition state could be obtained by a low multiplicity of infection with cytosine-substituting T4 phage. Thus it is now possible to attain a high yield of the trans-activated gene products. We discuss the advantage of the trans-activated T4 uvsY regulatory signal for production of foreign products.

Amino Acid Sequence↗
PubMed1991

A novel expression system for production of a labile protein in Escherichia coli by infection with cytosin-substituting T4 phage.

A novel expression system was developed for the high level production of a labile protein in Escherichia coli. The regulatory signal of bacteriophage T4 uvsY gene was fused in frame with the coding region of human ventricular myosin alkali light chain (VLC1) gene. Expression from the regulatory signal was enhanced and continued in a lysis-inhibition state by infection with a cytosine-substituting T4 phage mutant. VLC1 protein was produced at a low level without infection because of its instability in the cells. Although the productivity was partly improved in a lon-deficient mutant without infection, it was improved about 100-fold with T4 phage infection. T4 phage produces protease inhibitor(s) (pin gene product) against proteases of host cell including the lon gene product (protease La).

Amino Acid Sequence↗
PubMed1991

[Multidisciplinary therapy after hepatectomy for hepatocellular carcinoma from the view point of tumor recurrence].

This study was performed to elucidate the risk factors of early recurrence after hepatectomy for the 112 patients with hepatocellular carcinoma, excluding absolute non-curative resections and operative deaths, and effects of early prophylactic management were evaluated. In Cox's multivariate analysis, 3 pathological factors of portal vein invasion, intrahepatic metastasis and capsule formation or capsule infiltration as well as the nuclear DNA ploidy pattern were significantly correlated to postoperative recurrence. The patients with 2 or more pathological factors or with DNA aneuploid tumors developed recurrence at early postoperative period. However, even in the DNA aneuploid tumors, the patients with high DNA index and low S-phase fraction showed relatively better prognosis. Argyrophilic proteins of nucleolar organizer regions (AgNOR) were also correlated well to nuclear DNA analysis, showing one of the good indicators for estimating biological malignant nature. Therefore, intra-arterial regional chemolipiodolization therapy should be performed for these high risk patients at early period after hepatectomy. With these managements we have been getting much lower incidence of early recurrence.

Carcinoma, Hepatocellular↗
PubMed1991

Retarded cerebral growth of hormone-deficient mice.

1. Both the Snell dwarf mouse (dw) and the growth hormone-deficient Little mouse (lit) exhibit a microcephalic cerebrum with hypomyelination, retarded neuronal growth, and underdevelopment of axons and dendrites. 2. The hypomyelination is due to arrested glial proliferation, suggesting that the action of growth hormone on the proliferation and maturation of both glial and neuronal cells is a necessary precondition of myelin formation, apart from the complementary or synergistic actions of thyroxine. 3. In contrast, the cerebral hypomyelination present in the inherited hypothyroid mouse (hyt) is not related to arrested glial proliferation, demonstrating that thyroid hormones can act independently on myelinogenesis.

Animals↗
PubMed1991

[A comparison of three drugs (pethidine, magnesium sulfate and droperidol) in patients with post-anesthesia shivering].

Shivering is one of the common complications after general anesthesia. It increases metabolic rate markedly and may cause myocardial ischemia in patients with decreased cardiopulmonary function. We investigated, therefore, the effects of three drugs (pethidine, magnesium sulfate and droperidol) on shivering in 30 patients under enflurane anesthesia. The patients were of ASA class I or II physical status and had no cardiovascular or respiratory disease. For post-anesthesia shivering, every patient was administered with pethidine 0.5 mg.kg-1, MgSO4 30 mg.kg-1 or droperidol 0.15 mg.kg-1. Their VO2 and VCO2 were measured for 5 minutes during shivering and 15 minutes after drug administration. We also evaluated the therapeutic efficacy of each drug for the treatment of shivering by examining VO2 and VCO2 before and after drug administration. With pethidine and MgSO4, both VO2 and VCO2 decreased significantly after each drug administration. With pethidine, VO2 and VCO2 decreased significantly more than that in MgSO4 (P less than 0.01 vs P less than 0.05). But with droperidol, there was no significant change. These results suggest that pethidine is most effective followed by MgSO4.

Adult↗
PubMed1991

A human skeleton from the Ohguruwa remains.

In 1941, the Ohguruwa remains were discovered at the Mizuho sports ground site in Nagoya. They date from about 3000 B.C., which is the early Jomon era. When the stadium was reconstructed in 1980, four human skeletons were found. Three of them, however, were in poor condition and moreover, were incomplete. However, the second skeleton was in good condition and could almost be reconstructed, and this skeleton (No. 2) was used for our study. It was found in the classic posture with arms and legs folded. Some pieces of a dog's skeleton were also discovered near the No. 2 skeleton's chest. This particular skeleton generally showed characteristics typical of the Jomon era. It had a stout structure and was judged to be a middle aged male because of the following features. There was considerable attrition of the occlusal surfaces on the remaining teeth. The teeth were worn flat, probably owing to the hard food and to their use as a tool. Both canines and first premolars of the upper and lower arches had been extracted in accordance with the custom of the time. Typical caries and localized periodontal breakdown were not observed, although there was horizontal alveolar bone loss, especially in the anterior regions. The mastoid process was extremely large and prominent. The lateral prominence of the mandible was developed. Analysis of lateral cephalogram revealed that the mandible was in the anterior position. The angles of SNA, SNB and ANB were 89.6 degrees, 89.2 degrees and 0.4 degrees, respectively. The skeletal pattern was definitive Class III. The adaptive changes in the teeth, their supporting tissues, temporomandibular joints and the related muscles--the harmonious masticatory system--were all estimated.

Cephalometry↗
PubMed1991

Genetic hypothyroid mice: normal cerebellar morphology but altered glycerol-3-phosphate dehydrogenase in Bergmann glia.

The present study was undertaken to investigate the effects of thyroid deficiency on cerebellar development with mouse endocrine genetic models. Four types of mutant mice, the growth hormone- and thyroid hormone-deficient Snell dwarf mouse (dw/dw), the growth hormone-deficient little mouse (lit/lit), the primary hypothyroid mouse (hyt/hyt), and the congenital genital goiter mouse (cog/cog) were analyzed for expression of the glial enzyme marker glycerol-3-phosphate dehydrogenase (GPDH) and several other marker proteins. GPDH expression, as determined by enzyme activity and Northern blot analysis, was reduced by about 50% in the cerebellum and brainstem of the three hypothyroid mutant mice. No reduced expression was found in any region of the brain of the growth hormone-deficient lit/lit mutant. Visualization of GPDH by immunohistology showed that the immunoreactive enzyme was strikingly reduced in the Bergmann glial cells of dw/dw, hyt/hyt, and cog/cog mutant mice, particularly in the radial glial processes. To evaluate the specificity of the effect on GPDH expression, we also examined the expression of the glial cell-specific S-100 protein by immunohistology. In all mutant cerebella, both the intensity and pattern of staining of the Bergmann glial cells were indistinguishable from that of normal controls, suggesting that the Bergmann glial cells are morphologically normal in the hypothyroid mice. The morphology of the Purkinje cell neurons was similarly visualized by immunohistology using an antiserum specific for the microtubule-associated proteins. Surprisingly, the morphology of the Purkinje cell dendritic arborization also appeared unaltered in the hypothyroid mice. The results suggest that the morphological development of the mouse cerebellum is relatively unaffected by hypothyroidism.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1991

Constitutive expression of FosB and its short form, FosB/SF, induces malignant cell transformation in rat-1A cells.

We have established Rat-1A cell lines constitutively expressing c-Fos and the two products of the fosB gene, FosB and its short form, FosB/SF. The expressed proteins in the different stable transfectants have been characterized by immunofluorescence and immunoprecipitation analysis. Our results demonstrate that constitutive expression of FosB, like the constitutive expression of c-Fos and, to a lesser extent, FosB/SF, results in cells that grow to increased saturation densities and have the ability to grow in an anchorage-independent manner. Most important is the finding that expression of these proteins augments the tumorigenic potential of Rat-1A cells. These results show that both forms of FosB have a stimulatory effect on cell proliferation.

Animals↗
PubMed1991

[Effects of various forms of gastric surgery on gut hormones and pancreatic secretions].

Effects of various forms of gastric surgery on gut hormones and pancreatic secretions were examined using canine models. These operative procedures included simple laparotomy (group A; n = 13), truncal vagotomy with pyloroplasty (B; n = 17), selective proximal vagotomy (C; n = 17), proximal gastrectomy with pyloroplasty (D; n = 6), proximal gastrectomy with truncal vagotomy and pyloroplasty (E; n = 7), and distal gastrectomy (F; n = 19). The mean fasting serum gastrin and secretin levels (pg/ml) were 71.0, 82.5 in A, 94.0, 97.7 in B, 62.1, 108.1 in C, 58.2, 123.0 in D, 91.2, 138.6 in E, and 50.9, 74.5 in F, respectively. The mean value of plasma pancreatic glucagon (pg/ml) showed 73.6, 109.9, 106.8, 47.2, 37.8, and 74.5 in each of the six groups. Significant correlations were observed between values of serum lipase and those of serum gastrin as well as between the amount of pancreatic secretions and serum secretin levels. Pancreatic secretions were decreased markedly in group F and moderately in B. Basal tissue blood flow measured by hydrogen clearance method was low in D, E, and F when compared with that in A.

Animals↗
PubMed1991

[Clinical and experimental studies on pancreatic functions and gastrointestinal hormones after major pancreatectomy].

The present study was undertaken to evaluate the changes of gastrointestinal hormones and pancreatic functions after major pancreatectomy in dogs and human. After more than 92% pancreatectomy in dogs, all dogs developed diabetes mellitus (DM). In these dogs, the remnant pancreas showed poor regeneration rate of 24.0% at 6 weeks, decreased sigma IRI in IV-GTT had not been recovered, pancreatic glucagon response was diminished, pancreatic exocrine function had been declined with increased plasma levels of secretin and CCK, and gastric secretion increased in spite of diminished gastrin response. After 74 to 92% pancreatectomy, 17.6% of dogs developed DM. The dogs with DM had poor pancreatic regeneration rate of 22.7% at 12 weeks, hypersecretion of glucagon and decreased gastric secretion with low plasma concentration of gastrin. On the other hand, in dogs without DM, pancreatic regeneration rate showed 42.7% at 12 weeks, insulin release and pancreatic exocrine function had been recovered well, and plasma CCK levels increased without changes of gastric secretion. In the clinical study, gastric secretion, CCK response and pancreatic endocrine and exocrine functions had been maintained better after pylorus preserving pancreaticoduodenectomy than after conventional pancreaticoduodenectomy.

Animals↗
PubMed1991

[The relationship between opsonin, endotoxin and chemical mediators in postoperative complications after surgery].

The role of various chemical mediators in the development of complications after major surgery was investigated. Phospholipase A2 activity (PLA2), and the levels of pancreatic secretory trypsin inhibitor (PSTI), polymorphonuclear leukocyte elastase (PMNE), thromboxane B2 (TxB2), 6-keto-PGF1 alpha (6-KF), leukotriene (LT) B4, C4, D4, interleukin-beta (IL-1 beta), tumor necrosis factor (TNF), and endotoxin (Et) in the serum were measured in 134 surgical patients of whom 36 developed postoperative complications. PLA2, arterial TxB2 and 6-KF showed significant changes in the patients with post-operative complications, associated with elevated Et levels. The majority of these patients had a significantly higher ratio of TxB2/6-KF. These results suggest TxB2 and 6-KF, and the TxB2/6-KF ratio are useful indices of outcome in critically ill patients with hepatic failure. Our findings revealed marked production of prostanoids in sepsis and indicate a severity of the complication in balance of the thromboxane/prostacyclin axis. It was also suggested that the opsonin and eicosanoid levels are closely related to the serum endotoxin level. LTB4, C4 and D4 were increased in the patients with postoperative sepsis or DIC, especially at the initial onsets. The increased levels of IL-1 beta or TNF were observed in some patients with postoperative complications, especially those with severe postoperative complications.

Bacterial Infections↗
PubMed1991

Spontaneous rupture of hemangioma of the liver: treatment with transcatheter hepatic arterial embolization.

A case of spontaneous rupture of a cavernous hemangioma of the liver with a successful hepatic resection after transcatheter hepatic arterial embolization is reported. Twenty-eight cases of spontaneous rupture of hepatic hemangioma have been reported in the medical literature. Each of the cases (19 adults and nine children) were reviewed, including the present one reported here. Surgical treatments were carried out on 20 patients, of whom only five survived. Ruptured hemangiomas ranged in size from 3.0 to 25.0 cm, and many were located on the inferior surface of the liver. Surgical resection should be considered for a large hepatic hemangioma located on the inferior surface of the liver if the tumor has a high probability of rupture. Once the hemangioma has ruptured, emergent hepatic resection is recommended for low-risk patients, whereas high-risk patients should receive transcatheter hepatic arterial embolization prior to elective hepatic resection.

Aged↗
PubMed1991

Application of renal microangiography to normal and diseased kidneys of cattle and mice.

Use of microangiography is now essential for the study of microcirculation in various organs. Renal microangiographic studies have been reported in rats, rabbits, dogs, human beings, and mice. However, we could not find any report on use of the technique in cattle, despite high incidence of renal disease in that species. The perfusion technique used in mice was improved over that of our previous report, and was applied to normal and diseased bovine kidneys. For the microangiographic technique, composition of the contrast medium, pressure of the injection, duration of perfusion, and washing of kidneys with heparinized saline solution before perfusion are important. In cattle, 1- to 2-mm-thick sections of the kidneys were generally necessary to observe renal vasculature: arcuate and interlobular arteries, afferent arterioles, and glomerular capillaries. In normal bovine kidneys, the angiographic and microangiographic findings were easily recognized as normal, compared with those of normal mice. In affected bovine kidneys, which histologically represented glomerulonephritis and pyelonephritis, angiography and microangiography revealed corresponding findings.

Angiography↗
PubMed1991

[Clinical effects of low-dose Ara-C in acute nonlymphocytic leukemia and myelodysplastic syndromes].

Forty-eight patients with acute nonlymphocytic leukemia (ANLL) and myelodysplastic syndromes (MDS) were treated with low-dose Ara-C regimen (LDAC) (10 mg/m2 or 10 mg/body subcutaneously every 12 hours). Complete remission (CR) was obtained in sixteen patients (33%) and partial remission (PR) in six (16%). Seven of eight patients with hypoplastic leukemia entered CR. However, LDAC was not effective in MDS and ANLL developing from MDS. The rate of CR was 20% in relapsed or refractory ANLL. Relapse was occurred in thirteen patients until now. The median duration of remission was 7 months (range: 3-20 months). Seven of the sixteen patients who achieved CR were received LDAC at the same dose for 10 days every month as a maintenance therapy. The duration of CR of these patients was shown to be longer than that of the patients without any maintenance therapy. Myelosuppression was observed in nearly all of them and the other clinical findings including cytogenetic analysis indicated cytotoxicity rather than differentiation as the mechanism of LDAC. LDAC was effective especially in hypoplastic leukemia and the maintenance therapy was found to prolong the duration of CR.

Adult↗
PubMed1991

[Burkitt's type ALL with numb chin syndrome as an initial manifestation].

A case of Burkitt's type ALL with numb chin syndrome as the initial manifestation is described. A 57-year-old Japanese male was admitted to our hospital in November 14, 1989 because of paresthesia at the chin and lower lip with diplopia and ptosis. Neurological examination revealed oculomotor paralysis of the right side and hypesthesia on the chin, lower lip and buccal mucous membrane. Laboratory findings showed increased leukocyte count. Bone marrow aspirate revealed hypercellular marrow with 92.3% leukemic cells which had vacuoles in the cytoplasm and surface marker of IgM, kappa type. The abnormalities of karyotype included t(8;14). He was treated with chemotherapy and radiation. His conditions were temporarily improved, but relapsed later and died in March 6, 1990. Leukemic infiltrations to the trigeminal nerve were found in autopsy. The relationship between lymphoid malignancies and numb chin syndrome was discussed.

Burkitt Lymphoma↗