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T Nishimura

Publications and source records attributed to T Nishimura.

At least 1,585 records · Page 88Linked to original sources
PubMed1980

[Activities of collagenolytic enzymes in the human ovary (author's transl)].

To elucidate the mechanisms of follicular rupture at ovulation in human, activities of collagenolytic enzymes were measured in the human follicles at various stages of development by using two kinds of synthetic substrates, alpha-N-benzoyl-DL-arginine-2-naphthylamide HCl and N-carbobenzoxy-glycyl-prolyl-glycyl-glycyl-prolyl-alanine. The result clarified that human ovaries did have two kinds of collagenolytic activities, one cathepsin B1 with its optimal pH 6.0, the other neutral collagenase with its optimal pH 7.5. To examine the subtle changes of these enzymatic activities in the follicles during ovulatory processes, the follicle wall was dissected into three parts, namely, the granulosal layer, the apical wall and the basal wall without the granulosal layer. Activity of neutral collagenase presented a continuous increase in the follicular wall, while in the granulosal layer its gradual depletion was observed. Cathepsin B1 revealed a significant drop of its activity in the apical wall around a preovulatory period. These results indicate an involvement of collagenolytic enzymes in the human ovulatory process.

Cathepsin B↗
PubMed1980

[Activity of vertebrate collagenase in the human ovary (author's transl)].

Participation of collagenolytic activities in the ovulatory process had been recently studied in the experimental animals, though their existence in the human ovary remains obscure. In the previous report, we demonstrated the collagenolytic activity by using synthetic substrates N-carbobenzoxy-Gly-Pro-Gly-Gly-Pro-Ala and the cathepsin B1 activity in human ovarian follicles. To investigate the activity for degradation of native collagen in the human ovary, we used 14C-labeled collagen prepared from young rat skin in the present study. Collagenolytic activity for synthetic substrate DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg OH supposed to be specific for vertebrate collagenase was also measured. Our results clearly demonstrated that there existed vertebrate collagenase activity and the collagenolytic activity measured by using DNP-peptide in the human ovarian follicles. The correlation coefficient between both enzymatic activities was 0.56. The DNP-peptide-degrading activity seemed to partly represent the collagenolytic activity from vertebrate collagenase. It was concluded that vertebrate collagenase besides collagenolytic activities did exist in the human ovary and they seemed to be involved in the degradation of collagen fibers in the follicular apex at the time of ovulation.

Animals↗
PubMed1980

[Laboratory and clinical studies of cefoperazone in pediatric field (author's transl)].

The authors have carried out the laboratory and clinical studies of cefoperazone (CPZ). The results were as follows: The sensitivity was estimated by plate dilution method on 26 strains of S. aureus, E. coli and K. pneumoniae, 25 strains of P. aeruginosa, 14 strains of Salmonella sp. and 9 strains of GM resistant P. aeruginosa isolated from patients. The distribution of sensitivity of S. aureus was 1.56 approximately 25 mcg/ml and the peak of distribution was 3.13 mcg/ml. The growth of 96.2% of E. coli was inhibited at concentration of less than 12.5 mcg/ml. The growth of 50.0% of K. pneumoniae was inhibited at concentration of less than 6.25 mcg/ml. The peak of distribution of P. aeruginosa was 12.5 approximately 25 mcg/ml (GM sensitive) and 12.5 mcg/ml (GM resistant). CPZ was given by drip infusion for 30 minutes at a single dose of 25 mg/kg to 2 children, and by drip infusion for 60 minutes at a single dose of 46.9 mg/kg to a child. The serum mean level of CPZ was 127.5 +/- 8.5 mcg/ml at 30 minutes, 30.5 +/- 7.5 mcg/ml at 1 hour, 23.5 +/- 3.5 mcg/ml at 2 hours, 10.5 +/- 1.5 mcg/ml at 4 hours and 6.8 +/- 2.4 mcg/ml at 6 hours after administration at a single dose of 25 mg/kg, respectively. The serum level was 102.0 mcg/ml at 1 hour, 32 mcg/ml at 2 hours, 14.5 mcg/ml at 5 hours and 12.5 mcg/ml at 7 hours after administration at a single dose of 46.9 mg/kg. Half-life time was 92 minutes. The mean urinary excretion rate was 32.0 +/- 7.3% in the drip infusion for 30 minutes up to 8 hours after administration. CPZ was effective in 6 of 8 cases with pediatric bacterial infections. No side effects were observed except for 1 case with elevation of GOT.

Age Factors↗
PubMed1980

[Cytochemical demonstration of 3 beta-hydroxysteroid dehydrogenase activity in human corpora lutea (author's transl)].

3 beta-hydroxysteroid dehydrogenase (3 beta-HDS) activity was demonstrated ultrastructurally in human corpora lutea obtained from the women aged 32-46 who were treated by the hysterectomy for various gynecological indications. The corpora lutea were removed from ovaries and cut into small pieces of 1 mm3. Specimens were incubated at 25 degrees C for 20 min. in the media containing dehydroepiandrosterone 0.6 mg, 0.1 M sodium citrate 0.3 ml, 5 mM potassium ferricyanide 1 ml, 30 mM copper sulfate 1 ml, N.A.D. 3.6 mg, sucrose 1.0 g and 0.1 M phosphate buffer (pH 7.2) 6 ml. For a control study, 1.5 mg cyanoketone (by the courtesy of Dr G.O. Pott), the specific inhibitor for 3 beta-HSD, was added to the media. The materials were fixed in 4% glutaraldhyde and 1% OsO4 and embedded in Epon 812. Electron dense reaction products of copper ferrocyanide were observed in smooth endoplasmic reticulum (sER) and in intercristal and outer space of mitochondria. In control studies, 3 beta-HSD activity were completely abolished. It is concluded that not only sER but also mitochondria has 3 beta-HSD activity in human corpora lutea. Furthermore, mitochondria seems to have capability to synthetize progesterone from cholesterol by itself.

3-Hydroxysteroid Dehydrogenases↗
PubMed1980

Autoantibodies to zona pellucida in infertile and aged women.

A total of 735 human serum samples were tested against isolated porcine ova for detection of autoantibodies to zonae pellucidae by the specific method reported previously using indirect immunofluorescence. Positive fluorescence was found in 13 of 175 samples (7.4%) from patients with unexplained infertility, in 2 of 122 samples (1.6%) from patients with proven etiology of infertility, but in none of 141 samples from age-matched control subjects (fertile women and men). There was an age difference in the incidence of positive reactions: 4 of 156 samples (2.6%) from women over 40 years old were positive in contrast to only 1 of 159 samples (0.6%) from women under 40 years old. Although autoantibodies to zonae pellucidae may be produced during the aging process, the observed high incidence in unexplained infertility suggests an etiologic association of the antibody with the disease when produced in younger women.

Absorption↗
PubMed1980

[Double-blind comparison of L-keflex and cephalexin (Keflex) in dental infections (author's transl)].

In order to evaluate efficacy and safety of L-Keflex (granule form of sustained release cephalexin), a double blind study comparing it with Keflex (capsule of regular cephalexin) was conducted in dental infections. Evaluable cases in adults for efficacy of the drugs were 196 consisting of 97 for L-Keflex and 99 for Keflex. Those in children were 19 (8 for L-Keflex and 11 for Keflex). There were no significant differences in background of the patients and severity of the diseases between both groups (L-Keflex and Keflex groups). The daily doses used in both groups were 1,000 mg in adults and 500 mg in children, respectively. The dose was given in two divided doses for L-Keflex group and in four divided doses for Keflex group. Following are evaluation by the committee members for the study: Adults 1. Clinical response rate at final therapy day was 93.8% in L-Keflex group and 92.9% in Keflex group, showing no significant difference between both groups. 2. No significant difference in severity of subjective and objective symptoms between both groups was observed at each therapy day. 3. Side effects were found in 6.7% of 105 patients receiving L-Keflex and in 5.6% of 107 patients with Keflex, and there was no significant difference between both groups. As the side effects, gastrointestinal symptoms, rash and itching were observed, but no any other side effects were found in both groups. Children 1. As shown in the above, number of the cases enough to evaluate statistically was not obtained, but all of both groups clinically responded to the drugs. 2. As for side effects diarrhea was observed in only one patient of Keflex group consisting of 12 patients. In the patient, however, discontinuation of the drug was not required and the side effect disappeared during the therapy. From the above results, L-Keflex (granule) is judged to have more convenience than Keflex (capsule) in that (1) it can be administered with b.i.d. regimen and (2) it can be easily taken in dental patients such as patients having difficulty in opening mouth of swallowing pain.

Adolescent↗
PubMed1980

[An ultrastructural study of primary carcinoma of the fallopian tube (author's transl)].

Primary carcinoma of the fallopian tube is one of the least common neoplasms arising from the female genital tract with rare preoperative correct diagnosis. Recently, we had chances to experience two cases of this tumor, one of which was examined electron microscopically. Both of them revealed the well-differentiated pattern of papillary adenocarcinoma. Electron microscopically, most of the cancer cells had pleomorphic nuclei, giant or multiple nucleoli, numerous mitochondria, poorly developed rER and abundant polysomes. Small number of cells rich in lysosome-like granules were also observed near the basement membrane. Among the cancer cells, "ultrastructural alveolar spaces", composed of several cancer cells, were prominent. In the cancer cells, there were observed the following interesting observations; annulate lamellae-like structure, unusual bundles of the filamentous elements and lamellar body-like structures. Normal tubal epithelium consisted of ciliated cells, secretory cells, peg cells and so-called "indifferent cells". The fact that the present tumor had no ciliated cells and few secretory cells indicated that this tumor was originated from "indifferent cells" or peg cells.

Adenocarcinoma, Papillary↗
PubMed1980

[The effect of human cord plasma on the incorporation of 3H-thymidine by PHA-stimulated peripheral blood lymphocytes of cervical cancer patient (author's transl)].

It has been proposed that human cord plasma can suppress the PHA response of adult lymphocytes. To compare the suppressive effects between plasma from cancer patients and human cord plasma, we investigated the PHA responses of lymphocytes which were sampled from patients with various stages of cervical cancer and cancer free women, by adding cancer auto-plasma, human cord plasma and control plasma. The PHA responses of normal lymphocytes cultured with plasma from patients with 2nd and advanced stage of cancer were suppressed, but they were not suppressed by the plasma from O-I stage of cancer. The addition of control plasma enhanced PHA responses of lymphocytes of the 2nd and advanced stage of cancer, but no changes were observed in the lymphocytes from O-I stage of cancer. These results suggest that inhibitory factor does not appear in the plasma from the patients with O-I stage of cervical cancer. Human cord plasma moderately suppressed the PHA responses of normal lymphocytes; it also suppressed the PHA responses of lymphocytes from O-I stage of cancer. However, the PHA responses of lymphocytes from 2nd and advanced stage of cancer were improved significantly. Cord plasma may have a factor which promotes the PHA responses of lymphocytes from advanced cancer.

Adult↗
PubMed1980

[An ultrastructural study of ovulatory changes in stromal tissue of the follicular apex in the human (author's transl)].

To elucidate the mechanism of follicular rupture at ovulation in human, ultrastructural changes of stromal tissue of the follicular apex during follicular growth and ovulation were studied by focusing on the collagen and fibroblasts in the stroma. Sixteen ovaries were obtained from women at their thirties who underwent the abdominal hysterectomy for gynecologic diseases. From follicles at various stages of development, apical portions were excised and processed for electronmicroscopy. At the apex of growing follicles, fibroblasts with poor cytoplasm and abundant collagen fibers among them were observed in the layers of tunica albuginea and theca externa. At the preovulatory follicles, fibroblasts had rich cytoplasm containing lysosomal granules and multivesicular structures and intercellular collagen fibers were rather sparse. These lysosomal granules and multivesicular structures in the fibroblasts were much conspicuous in the theca external layer of preovulatory and post-ruptured follicles and collagen fibers among these fibroblasts were almost disappeared. It seems that these intercellular collagen fibers and ground substance were digested by the content of lysosomal granules and multivesicular structures.

Adult↗
PubMed1980

[A study of the effect of dihydrotestosterone on cultured human fibroblasts--with special reference to the hormone effect on the synthesis of proteins, collagen and DNA in fibroblasts (author's transl)].

Fibroblasts grown from abdominal and vulvar skin from normal women were tested for their dihydrotestosterone binding activity. Fibroblasts from either source had 8S cytoplasmic dihydrotestosterone receptors as confirmed by sucrose gradient analysis and the concentration of dihydrotestosterone binding sites and the apparent dissociation constant were 21.6 +/- 1.1 fmoles/mg protein and 0.50 +/- 0.028 nM, respectively, for fibroblasts from abdominal skin and 35.9 +/- 1.7 fmoles/mg protein and 0.24 +/- 0.015 nM, respectively, for fibroblasts from vulvar skin. The dihydrotestosterone binding activity was greater in fibroblasts from vulvar skin than from abdominal skin (P less than 0.01). Fibroblasts formed dense monolayers were treated with various concentrations of dihydrotestosterone (10, 100, 1,000 ng/ml) for six days and were assessed for hormone effect. The production of proteins was elevated in fibroblasts from either source with a slightly greater hormonal response occurring in fibroblasts from vulvar skin than from abdominal skin. Collagen production was elevated significantly in fibroblasts from vulvar skin but only marginally in fibroblasts from abdominal skin. The synthesis of DNA was not affected in fibroblasts from either source. These results than response to dihydrotestosterone was greater in fibroblasts with a higher hormone binding activity may reflect the hormone effect being mediated through dihydrotestosterone receptors.

Cells, Cultured↗
PubMed1980

[Determination of fetal sexes by ultrasonic tomography (author's transl)].

A newly developed manipulation of ultrasonic tomography has been successfully applied for determining the fetal sexes, based on the findings obtained by preliminary studies on the neonatal pelvic and external genitalia. The results are as follows: 1) In neonates, distinct sex differences in length and shape of the anoperineogenital (APG) area were observed by two-dimensional ultrasonic tomography. Transverse tomography of superficial portion of APG gave different images of M for males and of F for females. Frontal tomography produced images of the scrotum and the major labia. 2) In fetuses, distinction in the M and F images of APG between the both sexes was constantly recognized on the 20th week or later. The images representing the triangle formed by the bilateral tuber-ossis ischii and the ventral end of the external genitalia was found quite useful in determining the sexes between the 15th and 20th week of gestation. 3) Determination of fetal sexes in 604 pregnancies (including 4 pairs of twins) between 23th and 39th week of gestation turned out correct in 99.8% of the cases as compared with the sexes after their deliveries. 4) Although feasibility of this method exceeded 50% of the cases of 18th week of gestation, the optimal term for the determination of fetal sexes was estimated between 23 and 32 weeks of gestation.

Female↗
PubMed1980

[A cytochemical study of acid-phosphatase activity in follicular granulosa cells of human ovaries (author's transl)].

In order to clarify the nature and possible roles of lysosome-like granules observed electronmicroscopically in the granulosa cells of human preovulatory follicles, lysosomal activities in human preovulatory granulosa cells were investigated ultrastructurally by staining a marker enzyme acid phosphatase with modified Gomori technique. In the granulosa cells of follicles 10 approximately 15 mm in diameter, acid phosphatase reaction products were seen only in the Golgi apparatus. In follicles around 20 mm in diameter with extremely thin apical walls, granulosa cells with abundant nexuses and steroidogenic organelles (sER, large spherical mitochondria with tubular cristae and lipid-droplets), had many lysosome-like granules in the perinuclear regions and/or close to lipid droplets. Acid phosphatase reaction products were observed mainly in these granules, and infrequently in Golgi apparatus. These morphological results not only identify such granules as lysosomes, but also indicate that the granulosa cells are rich in the lysosomal activity. Possible roles of lysosomes in preovulatory granulosa cells deduced from the present results are as follows, 1) a role in steroidogenesis or in steroid-secretion, 2) an involvement in the ovulatory process with their proteolytic enzymes.

Acid Phosphatase↗
PubMed1980

[Sites of androgen overproduction in the polycystic ovary (author's transl)].

T elucidate the possible intraovarian site for androgen overproduction in the polycystic ovary (PCO), we investigated relative contribution of follicular and stromal compartments in steroidogenesis by in vitro experiments. The largest follicle and stoma were dissected from the PCO tissue obtained by wedge resection. A portion of each tissue was taken for histology and slices of each tissue were incubated with acetate-1-14C for 3 hours. Incorporation into progestins, androgens and estrogens was assessed by the reverse isotope dilution technique. Qualitatively, a predominant incorporation into androstenedione (76-88%) with little incorporation into estrogens (0.6-2.5%) was commonly observed in the follicle with marked hyperthecosis found in 3 typical PCOs, whereas a lesser incorporation into androstenedione (51-57%) with a slight increase into estrogens (6.4-14.6%) was verified in the follicles with mild hyperthecosis in 2 atypical PCOs. Quantitatively, actual incorporation into androstenedione was the greatest in the typical PCO follicles, followed by the atypical PCO follicles and the least or almost negligible in the stroma of PCOs. We concluded that atretic follicles rather than the stroma are principal sites of androgen overproduction by PCOs, and the androgenic capacity is related positively to the grade of hyperthecosis.

Adult↗