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Biomedical subjects

T Nishida

Publications and source records attributed to T Nishida.

At least 775 records · Page 43Linked to original sources
PubMed1971

Effect of hemoglobin concentration on the oxidation of linoleic acid.

The inhibitory effect of high concentrations of hemoglobin on the oxidation of linoleic acid was related to the ability of hemoglobin to associate with the fatty acid. Ultracentrifugation of the mixture of hemoglobin and potassium linoleate revealed that approximately 880 moles of linoleic acid could associate with 1 mole of hemoglobin. High concentrations of hemoglobin apparently reduced the amount of free linoleic acid accessible to the heme group, thus preventing the oxidation of the fatty acid. With low hemoglobin concentrations, at which the oxidation began immediately after the addition of the catalyst, the amount of free linoleic acid was considerably greater than that of bound linoleic acid.

Chemical Phenomena↗
PubMed1968

Effect of phospholipase A treatment of low density lipoproteins on the dextran sulfate--lipoprotein interaction.

The effect of phospholipase A on the interaction of low density lipoproteins of the S(f) 0-10 class with dextran sulfate was studied in phosphate buffer of pH 7.4, ionic strength 0.1, by chemical, spectrophotometric, and centrifugal methods. When low density lipoproteins that had been treated with phospholipase A were substituted for untreated lipoproteins, the amount of insoluble dextran sulfate-lipoprotein complex formed was greatly reduced. Hydrolysis of over 20% of the lecithin and phosphatidyl ethanolamine constituents of the lipoproteins prevented the formation of insoluble complex. However, even the lipoproteins in which almost all the phosphoglycerides were hydrolyzed produced soluble complex, which was converted to insoluble complex upon addition of magnesium sulfate. It is apparent that the lipoproteins altered extensively by treatment with phospholipase A retain many characteristic properties of native low density lipoproteins. Fatty acids, but not lysolecithin, released by the action of phospholipase A interfered with the formation of insoluble complex; this interference was due to association of the fatty acids with the lipoproteins. With increases in the concentration of the associated fatty acids, the amounts of magnesium ion required for the conversion of soluble complex to insoluble complex increased progressively. Charge interaction is evidently of paramount importance in the formation of sulfated polysaccharide-lipoprotein complexes.

Animals↗