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Biomedical subjects

T Naruse

Publications and source records attributed to T Naruse.

At least 307 records · Page 17Linked to original sources
PubMedDate not supplied

Incidence of thyroid cancer in Japan.

The incidence of thyroid cancer was analyzed based on data provided by cancer registration in Japan and also based on data for 11,104 cases of thyroid cancer registered at the Japanese Committee for Registration of Malignant Neoplasm of Thyroid (JCR-MNT) from 1977 through 1986. Incidence rates for men and women gradually increased over the study period from 1959 through 1985. The age-adjusted rates for 1985 showed 1.1 per 100,000 for men and 3.1 per 100,000 for women. The higher rates were observed in Hiroshima and Nagasaki cities. Male to female ratio was 1:6 in papillary and follicular carcinomas and 1:2 in medullary and anaplastic carcinomas. Papillary, follicular, and medullary carcinomas are more common in the fifth and sixth decades of life while anaplastic carcinoma is more common in the elderly. The age-specific rates by histological classification showed no change over the study period of 1977 through 1986. The increased incidence could be attributed to improvements in diagnostic procedures.

Adenocarcinoma↗
PubMedDate not supplied

Embryology of the upper limb: the molecular orchestration of morphogenesis.

Accumulating data on the molecular interactions that occur during limb development have greatly enhanced our understanding of the process of limb morphogenesis. In this chapter, the key morphologic events are described, the broad categories of molecules involved are defined, the known molecular cascades and specific pathways that orchestrate limb development are reviewed. In addition, cascades disrupted by known genetic mutations associated with limb malformations are identified.

Animals↗
PubMedDate not supplied

Detection of VP-16-treated HL-60 cell apoptosis by TUNEL electron microscopy.

Apoptosis is essential to many physiological processes, including maturation of cells and the immune system. Deficient regulation of apoptosis may play an important role in many pathological conditions, such as autoimmunity, AIDS, and myelodysplastic syndrome. Several methods have been described to identify apoptotic cells. DNA strand breaks can be identified by labeling free 3'-OH termini with modified nucleotides by enzymatic reaction (TUNEL method). In this study, apoptosis was introduced in cultured HL-60 cells treated with VP-16, and the TUNEL method was adapted for electromicroscopy, called the EM TUNEL method. The results of the EM TUNEL method were compared with those of light microscopy and flow cytometry. Apoptotic cells following VP-16 (10 micrograms/mL) treatment were detected after 3 h by all methods (light microscopy, Erythrocin B, electron microscopy, flow cytometry, TUNEL, and EM TUNEL). EM TUNEL was the most sensitive method of detection, with a detection rate of 32%. Furthermore, EM TUNEL was more suitable for distinguishing cell lineage than light microscopy TUNEL. The results indicate that EM TUNEL can be used to detect apoptosis in bone marrow species in vivo.

Apoptosis↗
PubMedDate not supplied

Familial nephrotic syndrome with focal glomerular sclerosis.

Two brothers with nephrotic syndrome are reported. Their parents were first cousins. The nephrotic syndrome developed at the age of 14 and 15 years and was resistant to the treatment with corticosteroid. The elder patient fell into renal failure about 2-1/2 years after the development of nephrotic syndrome, and the younger has demonstrated a progressive decrease in renal function. The renal histopathology in one patient revealed findings typical of focal glomerular sclerosis. In light of the clinical similarities in these two patients, it is likely that they have a familial form of focal glomerular sclerosis.

Adolescent↗
PubMedDate not supplied

Superior mesenteric varices and splenic renal shunt with portal hypertension: a case report of liver cirrhosis with complications.

The case of a 58-year old woman with cirrhosis complicated by hepatocellular carcinoma, hepatic encephalopathy and superior mesenteric varices is described. Angiography revealed large superior mesenteric varices and it was demonstrated that the portal venous blood was shunted into the right internal iliac vein via the right ovarian vein. Clinically, this patient had hepatic encephalopathy and hyperammonaemia, but no oesophageal varices were discovered on endoscopy. The hepatic encephalopathy suggested that the portal blood containing ammonium from the superior mesenteric vein entered the brain through the systemic vein without the ammonia being metabolized by the liver.

Carcinoma, Hepatocellular↗
PubMedDate not supplied

Successful treatment of concomitant acute myeloblastic leukaemia and adenoid cystic carcinoma of the palate.

The case of a 55-year old man who was affected simultaneously by acute myeloblastic leukaemia (AML) and adenoid cystic carcinoma of the palate is reported. The carcinoma was removed after induction chemotherapy for AML and the patient subjected to consolidation therapy. No reports are evident of other cases in which acute leukaemia and other malignancies were treated simultaneously, and in which the patient was cured of both. It is suggested that this case may serve as a reference for future similar cases.

Antineoplastic Combined Chemotherapy Protocols↗
PubMedDate not supplied

Rhabdomyosarcoma in a patient with mosaic Klinefelter syndrome and transformation of immature teratoma.

A 27-year-old man was found to have a mediastinal tumour and the histological diagnosis was immature teratoma. Remission was achieved by chemotherapy and total resection. However, he developed anaemia and leukoerythroblastosis after 2 years of remission, and was referred to our hospital. Rhabdomyosarcoma cells were detected in the bone marrow and pleural effusion. Moreover, karyotype analysis of peripheral blood and bone marrow cells revealed mosaic-type Klinefelter syndrome. We diagnosed the case as transformation of teratoma into rhabdomyosarcoma in Klinefelter syndrome. Although intensive chemotherapy was performed, the patient died with meningeal infiltration.

Adult↗
PubMedDate not supplied

Aminopeptidase inhibitor ubenimex inhibits the growth of leukaemic cell lines and myeloma cells through its cytotoxicity.

We investigated the effect of ubenimex, which has been demonstrated to have immunomodulating activities, on the proliferation of human leukaemic cell lines (HL 60 and K 562, myelogenic lines, Jurkat, a T-cell line and RPMI 8226, a multiple myeloma cell line) and on human bone marrow mononuclear cells from haematopoietic malignancy patients with acute myeloblastic leukaemia, chronic lymphocytic leukaemia or multiple myeloma. The growth of the myeloid cell lines and of a multiple myeloma cell line, but not the T-cell line, was significantly inhibited by cocultivation with ubenimex in a dose-dependent manner. The results of the experiment with haematopoietic malignancy cells showed inhibitory effects on tumour cells from patients with acute myeloblastic leukaemia and multiple myeloma, but not on those from patients with chronic lymphocytic leukaemia. These findings suggest the usefulness of ubenimex for the treatment of multiple myeloma.

Aminopeptidases↗
PubMedDate not supplied

Effect of inhibitors of protein phosphatase 1 and 2A on erythroid colony formation: an investigation of the specificities of inhibitors.

To investigate the possible involvement of protein phosphatase (PP)1 and PP2A in the process of erythropoiesis, we assessed the effect of PP1 and PP2A inhibitors on erythroid colony formation using an in vitro colony formation assay. Okadaic acid (OKA), calyculin A (Cal-A) and tautomycin suppressed colony formation but 1-nor-okadaone did not. These results suggest that PP1 and PP2A both play an important role in erythropoiesis. Furthermore, higher concentrations of tautomycin were needed to suppress colony formation compared to concentrations of OKA and Cal-A. The target enzyme of inhibitors in erythropoiesis may be PP2A.

Antifungal Agents↗
PubMedDate not supplied

Electron microscopic analysis of myeloma cells in relation to drug response and prognosis.

Myeloma cells obtained from 33 patients were analyzed by electron microscopy to determine whether there is any correlation among ultrastructural characteristics of myeloma cells, drug response, and prognosis. The median survival time after diagnosis of responders (18 cases) and nonresponders (15 cases) was more than 423 days and 139 days, respectively. Nuclear immaturity and three cytoplasmic abnormalities--scattered pattern of mitochondria, single-sac looplike structures, and numerous intramitochondrial granules--were found to be associated with poor outcome. Although the cytoplasm of almost all the myeloma cells examined was judged to be mature or intermediate, the degree of nuclear immaturity was considered to correspond closely to the grade of nuclear-cytoplasmic asynchrony. Thus the electron microscopic examination of myeloma cells is of use to assess accurately their immaturity and abnormality and might provide clues for the prediction of drug response and prognosis of individual patients.

Female↗
PubMedDate not supplied

Clinical analysis of the prognostic value of ultrastructural abnormalities of plasma cells in patients with multiple myeloma.

The prognostic value of ultrastructural abnormalities of plasma, cells was analyzed in 61 patients with multiple myeloma treated in our hospitals from 1985 to 1996. The survival curves demonstrated significant differences by stratification of ultrastructural abnormalities, such as irregular contour of the nuclear membrane, nuclear body, budding, disarrangement of organelles, fibrillar formation, intramitochondrial granules, multilamellar body, single-sac loop-like structure, and vacuole. However, categorizing by nuclear inclusion body, cytoplasmic inclusion body and dense body showed no significant difference in survival curves. Abnormalities, as well as maturity, may be useful in predicting the prognosis of myeloma patients.

Bone Marrow Cells↗
PubMedDate not supplied

Ultrastructural abnormalities of bone marrow erythroblasts in refractory anemia.

Ultrastructural abnormalities of erythroblasts in 30 patients with refractory anemia (RA) according to FAB classification were studied. Nuclear clefts, iron-laden mitochondria, and myelin figures in cytoplasm were most frequently observed. Eleven patients (36.7%) with nuclear clefts of erythroblasts had a higher platelet count and higher incidence of cytogenetic abnormalities linked to poor prognosis than the patients without nuclear clefts. They also had a higher frequency of leukemic transformation and shorter survival than the patients without nuclear clefts. The nuclear clefts of erythroblasts, which are the result of impairment of DNA metabolism or nuclear formation and fusion during mitosis, may be one of the adverse prognostic factors for patients with RA.

Adolescent↗
PubMedDate not supplied

[Successful treatment with vincristine for an elderly patient with idiopathic thrombocytopenic purpura].

A 77-year-old female was referred to our hospital in March 1991 because of a severe bleeding tendency. Her blood count on admission was as follows: Hb 7.5 g/dl, WBC 4.6 x 10(9)/l with normal differentiation and platelet 2 x 10(9)/l. One month prior to admission, her blood count was normal. Initially, acute idiopathic thrombocytopenic purpura (ITP) was suspected, because of the acute onset of the bleeding tendency and thrombocytopenia. High dose intravenous immunoglobulin (400 mg/kg/day for 5 days) and bolus methylprednisolone (1 g/day for 3 days then tapered) were administered, starting March 13. Her platelet count had increased immediately on March 20 to 40 x 10(9)/l. However, platelet count decreased to 4 x 10(9)/l in the following two weeks. Her clinical course differed from that of typical acute ITP. Because the treatment with prednisolone was not effective, it was changed to intravenous infusion of vincristine (VCR) at a weekly dose of 1 mg for 6 weeks. The treatment was extremely effective, and her platelet count reached over 200 x 10(9)/l. The treatment was discontinued. Three weeks later, her platelet count decreased to 15 x 10(9)/l, the administration of VCR was resumed, and her platelet count recovered again. Throughout her clinical course, no side effect of VCR was noticed except for mild hypesthesia of the fingertips. VCR therapy was considered to be an useful treatment in elderly patients with ITP.

Aged↗
PubMedDate not supplied

Cross-dependence on ethanol and pentobarbital in rats reinforced on diazepam.

Cross-dependence on ethanol and pentobarbital was studied in rats reinforced on diazepam, using an intravenous self-administration method. Five rats were allowed to self-administer diazepam 2.0 mg/kg per injection intravenously by pressing a lever on a continuous reinforcement schedule over a 20 day period. The total daily dose of diazepam delivered reached 50 mg/kg/day. Thereafter, ethanol (50 or 100 mg/kg per injection) and then pentobarbital (6 mg/kg per injection) were substituted for diazepam for 3 and 2 days, respectively. During these substitution periods, responding for self-administration, food intake and body weight were recorded. When ethanol was substituted, self-administration responding increased and then decreased. Food intake and body weight also decreased during this period. These changes during the ethanol substitution session resembled those observed during withdrawal sessions. In contrast, when pentobarbital was substituted, no significant changes in self-administration responses, food intake or body weight were seen. These findings suggest that diazepam produces cross-dependence on pentobarbital, but not on ethanol at the doses used in this experiment.

Animals↗
PubMedDate not supplied

Antihypertensive effects of a new transdermal delivery system for clonidine (M-5041T) in spontaneously hypertensive rats.

The antihypertensive effects of a new transdermal delivery system for clonidine (clonidine tape; M-5041T) were investigated in spontaneously hypertensive rats and normotensive Wistar-Kyoto rats. In spontaneously hypertensive rats, M-5041T, at doses of 0.5-4.5 mg/kg (1.25-11.25 cm2/kg), produced a dose-dependent decrease of both systolic blood pressure and heart rate for 24 hr during transdermal application. These significant hypotensive effects lasted for 24 hr following the patching of M-5041T on the back of the animals at a dose of 4.5 mg/kg. In normotensive Wistar-Kyoto rats, the hypotensive and bradycardic effects of M-5041T were weak compared with those in spontaneously hypertensive rats. In both rat strains, M-5041T (4.5 mg/kg) caused behavioral changes such as sedation, piloerection and exophthalmos, accompanied by hypotensive effects. Both M-5041T (1.5 mg/kg) and clonidine (50 micrograms/kg, subcutaneously and 100 micrograms/kg, orally) induced similar hypotensive effects, accompanied by sedation. The hypotensive effects following transdermal M-5041T or systemic clonidine administrations were correlated with the time courses of the plasma clonidine concentration. In contrast to clonidine administered subcutaneously or orally, M-5041T produced a gradual increase in the plasma clonidine concentration, which persisted at a consistent level for at least 12 hr thereafter. Significant hypotensive effects lasted for 12 and 24 hr following M-5041T patching at 1.5 and 4.5 mg/kg, respectively. The present findings suggest that M-5041T can serve as an efficient and useful antihypertensive transdermal delivery system to achieve persistent blood pressure control.

Administration, Cutaneous↗