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Biomedical subjects

T Nakada

Publications and source records attributed to T Nakada.

At least 631 records · Page 35Linked to original sources
PubMed1981

Purification and properties of D-beta-hydroxybutyrate dehydrogenase from Zoogloea ramigera I-16-M.

D(-)-beta-Hydroxybutyrate dehydrogenase was purified from Zoogloea ramigera I-16-M to electrophoretic homogeneity. The molecular weight of the enzyme as determined by Sephadex G-200 gel filtration was 112,000, and the monomer molecular weight estimated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate was 28,000, indicating that the native enzyme is a tetramer with four identical subunits. The enzyme showed a pH optimum at 8.0 in the oxidation reaction, and a broad pH optimum (5.5-7.5) in the reduction reaction. The Km values for D(-)-beta-hydroxybutyrate and NAD in the oxidation reaction were 3.2 X 10(-4) M and 5.7 X 10(-5) M, respectively. The Km value for acetoacetate in the reduction reaction was 1.5 X 10(-4) M and that for NADH was 1.5 X 10(-5) M. Acetyl CoA, D-lactate, and 2-hydroxybutyrate were effective inhibitors for the oxidation of D(-)-beta-hydroxybutyrate. The enzyme was sensitive to the inhibitory actions of sulfhydryl reagents such as p-chloromercuribenzoic acid, 5,5'-dithiobis(2-nitrobenzoic acid) and HgCl2.

Electrophoresis, Polyacrylamide Gel↗
PubMed1981

Carcinoembronic antigen and endocrine response in a patient with Sipple's syndrome following surgery.

Circulating carcinoembryonic antigen (CEA), calcitonin (CT) and adrenocorticotropic hormone (ACTH) and levels in tissue extracts of a patients with Sipple's syndrome were measured simultaneously. Biofocal medullary carcinoma of the thyroid gland (MCT), left adrenal phaeochromocytoma and ectopic para-aortal phaeochromocytoma had been removed in the first operation. Total thyroidectomy was performed thereafter. Evidence has been presented showing that (1) the first operation lowered the elevated serum levels of CEA and CT, and levels of CEA and CT in tissue extracts of MCT were considerably higher than those of tumor-uninvolved thyroid tissue and phaeochromocytomas; (2) markedly elevated levels of plasma ACTH were normalized by the first surgery and, in addition, immunoreactive ACTH and beta-melanocyte stimulating hormone (beta-MSH) were detected in the phaeochromocytomas removed. This is a documented case of the simultaneous production of CT and CEA by MCT associated with ectopic secretion of ACTH and beta-MSH from the phaeochromocytomas without manifesting clinical features of Cushing's syndrome and is based on the findings referred to above.

Adrenal Gland Neoplasms↗
PubMed1981

The corrected protein equation to estimate plasma colloid osmotic pressure and its development on a nomogram.

Examining the internal discrepancies of three equations (Landis and Pappenheimer 1965) which represent the relationship between colloid osmotic pressures (COP) and normal human plasma proteins, the authors derived a corrected globulin equation: COPglb = 1.3c + 0.14c2 + 0.006c3 (COP: mmHg, c: protein content, g/100 ml), instead of their COPglb = 1.6c + 0.15c2 + 0.006c3, when A/G (albumin-globulin ratio) = 1.1 as they had described. Revising the former globulin equation again, based upon the current estimate of A/G = 1.8, we derived a new globulin equation. Our new plasma equation, consisting of their albumin equation and our revised globulin equation, is COPpl = alpha (2.8c + 0.18c2 + 0.012c3) + beta (0.9c + 0.12c2 + 0.004c3), where alpha and beta stand for albumin and globulin fraction, respectively. The last, being generalized for plasma with any protein compositions, and closely corresponded to the values directly measured in normal human plasma with variable protein compositions, we developed it on a nomogram for practical use.

Adult↗
PubMed1981

Primary position upbeat nystagmus. Another central vestibular nystagmus?

Recent studies of the vestibulo-ocular reflex have revealed a distinct pathway from the anterior semicircular canal to the contralateral oculomotor nucleus via the superior vestibular nucleus. Axons of this pathway ascend in the brachium conjunctivum, while axons of the other semicircular canal pathways ascend in the medial longitudinal fasciculus (MLF). We report two cases of primary position upbeat nystagmus where lesions of the brachium conjunctivum were suggested by computed tomography (CT) scans. One of these lesions was confirmed at autopsy. We concluded that primary position upbeat nystagmus, like downbeat nystagmus, is a type of central vestibular nystagmus resulting from an imbalance of vertical vestibulo-ocular reflex activity.

Adenocarcinoma↗