Search PubMed⌕ Search

Biomedical subjects

T Naguro

Publications and source records attributed to T Naguro.

25 records · Page 2Linked to original sources

The vomeronasal epithelia of NMRI mouse. A scanning electron-microscopic study.

The features of the apical and lateral surfaces of cells of the vomeronasal epithelium were studied in adult male mice by scanning electron microscopy. Supporting cells and receptor cells of the neuroepithelium are covered with microvilli. Microvilli of the sensory cells are longer and thinner than those of the supporting cells. Additionally, the former differ in local distribution, orientation, occurrence of branching and appearance of the cell coat. The receptor-free epithelium consists most likely of one cell type only, which shows different structural modifications including the presence, number and length of microvilli and cilia. In the transitional region, between the neuroepithelium and the receptor-free epithelium, immature receptor cells are present.

Animals↗

Effect of N-methyl-formimino-methylester on the vomeronasal neuroepithelium of mice.

N-methyl-formimino-methylester (MFM), a highly volatile chemical substance, causes massive, transient sensory-cell degeneration in the main, but not in the vomeronasal olfactory sensory epithelium of mice. After MFM-treatment it appears possible to study the accessory olfactory system after chemical "deafferentation" of the main system.

Animals↗

Polytene chromosomes observed scanning electron microscope.

Polytene chromosomes of the salivary gland cells from Drosophila melanogaster were observed by means of scanning electron microscopy using mainly the cracking method. Band and interband regions were visible as transverse striped patterns consisting of multiple, side-by-side fibres. Band regions seemed much more compact than interband ones. In the cross section of the entire chromosome, about 1,100 chromatid fibres could be counted. Intranuclear fine filaments could be recognized surrounding the polytene chromosomes each of whose diameters were almost the same (160--200 A). Field emission scanning electron micrographs showed the unit chromatid fibres clearly in very high magnification.

Animals↗

Scanning electron microscopic observation on intracellular structures of ion-etched materials.

Ion-etching technique on the cracked surface of biological material may give a plastic visualization of intracellular structures under the scanning electron microscope, because membraneous structures in the cell are generally etch-resistant and the cytoplasmic matrix is easily ion-etched. Mild ion-etching using low voltage was applied to the cells of the pancreas. Nuclear pores were clearly disclosed but were enlarged slightly in the process of etching. Endoplasmic reticulum with ribosomes, Golgi apparatus, mitochondria, some filamentous structures and crystalline inclusions (B cell granules) were also effectively disclosed by ion-etching technique. It is necessary, however, to compare the etched specimens carefully with non-etched ones to determine whether given structures observed are intrinsic ones or artifacts caused by etching.

Animals↗