[Experimental studies on membrane potentials in skeletal muscle in circulatory disorders].
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Action Potentials↗
Biomedical subjects
T Nagakura
Publications and source records attributed to T Nagakura.
[On the remote results of the operation of peripheral nerve injuries treated in our clinic].
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Adult↗
Tenascin-C levels in pseudosynovial fluid of loose hip prostheses.
OBJECTIVE: Aseptic loosening is one of the most important problems that can occur after total hip arthroplasty (THA). In this study, we analysed levels of large tenascin-C (TN-C) variants and compared them in pseudosynovial fluid from patients with aseptic loosening after THA with those in synovial fluid from patients undergoing primary THA (control). METHODS: Pseudosynovial fluid samples (n = 24) were obtained by aspiration at the time of revision THA performed due to aseptic loosening. Synovial fluid samples (n = 12) were obtained by aspiration at the time of primary THA. Expression of TN-C splice variants was examined using immunoblotting. TN-C levels were measured using an enzyme-linked immunosorbent assay (ELISA) system that we developed previously. RESULTS: Western blotting showed the presence of large TN-C variants in pseudosynovial fluid of artificial joints with loosening. TN-C levels were approximately three times higher in pseudosynovial fluid of loose artificial joints (median 151.9 ng/mL) than in synovial fluid controls (median 50.1 ng/mL) (p = 0.035). CONCLUSION: Levels of TN-C including large variant subunits are elevated in pseudosynovial fluid of loose artificial joints, indicating that TN-C is a useful novel biochemical marker of loose hip prostheses.
Aged↗
Drug effects on exercise-induced late asthmatic responses.
Disodium cromoglycate, prednisolone, beclomethasone and salbutamol were administered prior to the cycle ergometer exercise task separately to different groups of children with exercise-induced dual asthmatic reaction. Pre-treatment with disodium cromoglycate of ten asthmatics totally inhibited the dual reaction. Prednisolone treatment of eleven asthmatics and beclomethasone treatment of five asthmatics did not inhibit the early response but only the late response. Salbutamol treatment of ten asthmatics mainly inhibited the early phase reaction. Changes in neutrophil chemotactic activity correlated with airflow limitation in the disodium cromoglycate and prednisolone study, however, there were some discrepancies between the changes in FEV1 and neutrophil chemotactic activity in the beclomethasone and salbutamol studies. These findings suggest that exercise-induced dual asthmatic reaction are modified by disodium cromoglycate, prednisolone, beclomethasone or salbutamol in a mechanism which may be different in each drug and that there are similarities in the inflammatory responses in antigen- and exercise-induced dual asthmatic responses.
Albuterol↗
High molecular weight-neutrophil chemotactic activity in nasal allergy.
HMW-NCA is involved in the pathogenesis of nasal hypersensitivity reactions in humans. We, however, have little evidence about the role of neutrophils which migrate to the site of allergic reactions by HMW-NCA and other chemotactic mediators. This role of neutrophils in nasal hypersensitivity still remains to be clarified.
Chemotaxis, Leukocyte↗
Antidiuretic hormone in acute asthma in children: effects of medication on serum levels and clinical course.
This study of seventeen patients presenting to the emergency room with acute severe asthma determined levels of antidiuretic hormone in serum as well as serum electrolytes and arterial blood gases serially. There was a progressive increase in plasma ADH concentration with severe asthma and ADH levels were substantially higher in those patients with PaCO2's higher than 45 Torr. Patients treated with intravenous aminophylline had a fall in ADH levels while those treated with injectable epinephrine showed an elevation of these levels. As the asthma improved, ADH levels decreased in all patients. In this series of children, sick for not more than 24 hours, serum sodium levels were normal. However, children who have been ill for longer periods than this or who are on prolonged intravenous fluid therapy need close monitoring of serum electrolyte concentrations.
Adolescent↗
Drug effects on antigen-induced release of high molecular weight-neutrophil chemotactic activity and histamine in nasal secretion from children with allergic rhinitis.
Five children with allergic rhinitis sensitive to house dust mite as documented by skin test, radioallergosorbent test, score and nasal provocation were studied. Patients were subjected to nasal challenge with 3.125, 6.25, 12.5, and 25 protein nitrogen micrograms per disc of house dust mite antigens and nasal secretions were collected by the filter paper method. Neutrophil chemotactic activity (NCA) and histamine content of the nasal secretions were measured. NCA and histamine were released in a dose- and time-dependent manner, and no significant correlation between NCA and histamine was revealed in each subject. Pretreatment with 5.2 mg of nasal disodium cromoglycate or 0.5 micrograms of nasal adrenaline 5 minutes before provocation totally inhibited mediator release upon nasal challenge with 12.5 micrograms of house dust mite. A dose of 100 micrograms of nasal beclomethasone dipropionate 5 minutes before challenge neither inhibited clinical symptoms nor mediator release; 100 micrograms x 3 times per day of nasal beclomethasone dipropionate for 3 days, however, significantly inhibited clinical symptoms and release of both NCA (p less than 0.05) and histamine (p less than 0.05). NCA eluted as a single peak with an estimated molecular weight of 600,000. These results suggest that high molecular weight-NCA may be involved in the pathogenesis of allergic rhinitis.
Allergens↗