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Biomedical subjects

T Mura

Publications and source records attributed to T Mura.

27 records · Page 2Linked to original sources
PubMed1985

Measurement of the ratio of primary to total bile acids in serum by enzymatic fluorometric microassay and its clinical significance in patients with liver disease.

An enzymatic fluorometric microassay using 3 alpha- or 7 alpha-hydroxysteroid dehydrogenase was devised to determine serum total or primary bile acids. The present microassay using 50 microliter of a serum sample was run as a two-step method; the first step is the reaction of substrates with NAD+ and the hydroxysteroid dehydrogenase to produce as much NADH as possible in the incubation system and the second step is that of NADH with resazurin coupled with 1-methoxy phenazinemethosulfate to produce fluorescent resorfin. The assay is specific for 3 alpha- or 7 alpha-hydroxy bile acids, sensitive (a detection limit of 1.5 microM with a linear range of 1.5-80 microM), reproducible (CV = less than 5%) and inexpensive. As measured by this microassay, the ratio of serum primary to total bile acids (P/T ratio) in 10 normal subjects was 62.1 +/- 0.7% and was significantly elevated in the early phase of acute hepatitis (p less than 0.01), followed by return to the normal level after 4 weeks.

3-Hydroxysteroid Dehydrogenases↗
PubMed1984

Identification of trypsin inhibitor in bovine pituitary extracts as a survival factor for adult rat hepatocytes in primary culture.

Adult rat hepatocytes in primary culture cannot survive more than 2 days in the absence of calf serum. An extract of bovine pituitary gland had a similar effect to calf serum on the survival of hepatocytes, but its specific activity was 70 times that of calf serum. The survival factor was purified from bovine pituitary gland, and obtained in a homogeneous state judging by sodium dodecylsulfate-polyacrylamide gel electrophoresis and high performance liquid chromatography. Its purification was achieved by acid extraction of the pituitary gland, gel filtrations on Sephadex G-75 and Ultrogel AcA 202, ion-exchanger chromatography on CM-cellulose, and then reverse phase high performance liquid chromatography. The purified factor was effective at 10 ng/ml and maximally effective at 100 ng/ml. The overall recovery of its activity was 30%, and the specific activity of the purified factor was 3,100 times that in the acid extract. The molecular weight (Mr 8,000), estimated by Sephadex G-200 filtration, and the strong basic character (pI 10.5) of the purified survival factor suggested that it may be the trypsin inhibitor found in various tissues. Indeed, the amino acid composition of the pure material was identical with that of bovine pancreatic trypsin inhibitor (bPTI). The purified survival factor had the same inhibitory activity on trypsin as commercial bPTI and, conversely, commercial bPTI greatly enhanced survival of rat hepatocytes. Thus, the survival factor in bovine pituitary gland was identified as bPTI.

Amino Acids↗
PubMed1982

Gastric plasmacytoma: an immunologic and immunohistochemical study.

A case of IgG (lambda) secreting plasmacytoma arising in the stomach has been presented. Immunohistochemically, the tumor cells contained IgG-lambda immunoglobulin. Immunoelectrophoretic study on patient's serum revealed in the gamma zone the presence of M protein which reacted specifically with anti-IgG and anti-lambda antisera. Agar electrophoresis and immunofixation electrophoresis with anti-IgG and anti-lambda antisera showed multiple subbands. Neuraminidase treatment of the purified IgG from this patient resulted in gradual reduction of four subbands to two. Electrophoretic charge difference of the monoclonal immunoglobulin may be attributed to incompletely synthesized sugar chains attached to the protein core.

Blood Proteins↗
PubMed1981

Red cell preservation and removal system of purine derivatives.

The new preservative containing high concentrations of adenine (8 mM), inosine (40 mM) and other chemicals which was indicated by Nakao et al., was applied to the preservation of red cell concentrates. Moreover, the system for the removal of purine derivatives (adenine, inosine and hypoxanthine) added and produced in stored samples was developed, using bead activated charcoal coated with the blood-compatible polymer (Hydron). Over 90% of purine derivatives in the medium and erythrocytes were removed by Hydron-coated bead activated charcoal (HAC) column. The recovery of erythrocytes by HAC column treatment was over 98%.

2,3-Diphosphoglycerate↗