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T Mukai

Publications and source records attributed to T Mukai.

435 records · Page 25Linked to original sources
PubMedDate not supplied

Multidetector SPECT scanner for brain and body: system performance and applications.

A multidetector whole-body single photon emission computed tomography (SPECT) scanner has been developed for high-resolution dynamic SPECT imaging. The scanner consists of three detector rings with 128 NaI crystals (14 x 26 x 30 mm) in each ring, providing three tomographic images at 30 mm intervals. This system uses a rotation of collimator around the patient combined with the detector rotation for fine data sampling. The in-plane spatial resolution is 14.2 mm (full width of half maximum) with high-resolution (HR) collimator, 22.0 mm with high-sensitivity (HS) collimator, and 10.6 mm with high-resolution brain (HRB) collimator. The axial resolution is 24.0, 34.0, and 23.5 mm with HR, HS, and HRB collimator, respectively. The sensitivity measured with a 20% energy window for 99mTc in a 20 cm diameter phantom is 5.7, 17.0 and 6.6 kcps/muCi/ml with HR, HS, and HRB collimator, respectively. This scanner allows dynamic SPECT imaging both for brain and body organs with good spatial resolution.

Brain↗
PubMedDate not supplied

Transduction of CD34+ cells by a vesicular stomach virus protein G (VSV-G) pseudotyped HIV-1 vector. Stable gene expression in progeny cells, including dendritic cells.

OBJECTIVE: To use HIV-1 vectors to mediate stable gene transfer into hematopoietic stem/progenitor cells. STUDY DESIGN/METHODS: Purified human CD34+ cells were transduced with HIV-1 vectors pseudotyped with VSV-G and subjected to colony-forming assays and differentiation in liquid culture. Transduction was determined by DNA-polymerase chain reaction (PCR) for the transgene. GFP reporter gene expression and phenotypes of progeny cells were assessed by microscopy and flow cytometry. RESULTS: The HIV-1 vector transduced CD34+ cells with high efficiency. Transduction did not interfere with CD34+ cells differentiation in vitro. Transduced genes are expressed in different subsets of progeny cells, including those with normal dendritic cells (DC) morphology and phenotypes (HLADR+/CD1a+/CD86+/CD14-). CONCLUSIONS: We have demonstrated efficient transduction of hematopoietic progenitor cells by HIV-1 vectors. The transgenes are expressed in different subsets of progeny cells, which suggests stable integration. The generation of DCs stably expressing HIV antigens provides a new approach for vaccine development.

Animals↗