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Biomedical subjects

T Mizusawa

Publications and source records attributed to T Mizusawa.

26 records · Page 2Linked to original sources
PubMed1993

Value of urinary endothelin-1 in patients with primary vesicoureteral reflux.

By using a radioimmunoassay specific for endothelin-1 (ET-1), we measured urinary excretion of ET-1-like immunoreactivity (LI) in 63 spot urine samples of 48 patients with primary vesicoureteral reflux (VUR). And also, urinary excretion of N-acetyl-beta-D-glucosaminidase (NAG), beta 2-microglobulin (beta-2-MG), microalbumin (Alb) and creatinine (Cr) were measured. There was no significant correlation in any of the pairs ET-1 and NAG, ET-1 and beta 2-MG, and ET-1 and Alb. Comparing the grade of reflux according to the International Classification with urinary ET-1, urinary ET-1/Cr levels in patients with grade 2, 3 and 4 VUR were higher than normal, and the ratio of more than normal urinary ET-1/Cr increased in proportion to the grade of reflux, but it conversely decreased in grade 5. In conclusion, urinary ET-1 may be an indicator of renal tubular injury in patients with primary VUR, and its meaning may be different from conventional urinary parameters.

Acetylglucosaminidase↗
PubMed1993

Evaluation of upper urinary tract dynamics by diuresis renography in patients receiving urinary reservoir operation. Comparison of full and empty reservoir.

Upper urinary tract dynamics was evaluated using diuresis renography during full and empty reservoir in 14 patients (11 men, 3 women, 13-70 years old) with intestinal urinary reservoir or intestinal bladder augmentation. Types of operation were Mainz pouch urinary diversion 5, Mainz neobladder to urethra, 5, Kock pouch urinary diversion 2, and Mainz bladder augmentation 2. Diuresis renography was performed using 99mTc-DTPA and furosemide during full and empty reservoir, and several parameters (Tmax, T75, T50, GFR) in addition to the patterns of renogram were evaluated. In the normal control, none of the parameters of the diuresis renogram with full bladder differed from those with empty bladder. In 5 of 14 patients, abnormal waves on cystometry (CMG) were found, and in 4 of these 5 patients, the patterns of diuresis renogram of full reservoir were worse than those of empty reservoir. However, the patterns of diuresis renogram of full reservoir were not different from those of empty reservoir in 9 patients without abnormal waves on CMG. In conclusion, renal injury may easily occur in patients with intestinal reservoir and abnormal waves on CMG.

Adolescent↗
PubMed1993

Concentration of gadolinium-diethylene triamine pentaacetic acid in human kidney--study on proper time for dynamic magnetic resonance imaging of the human kidney on low and high magnetic fields.

Although Gadolinium diethylene triamine pentaacetic acid (Gd-DTPA) has been used as a contrast material in magnetic resonance imaging, it is known that contrast enhancement effect disappears if the concentration of Gd-DPTA increases beyond some levels. In this study, to evaluate the proper pulse sequences for dynamic magnetic resonance imaging (MRI) in the human kidney, the concentration of Gd-DTPA was quantitatively measured by inductively coupled plasma (ICP) emission spectrometry in human biological samples after administration of Gd-DTPA, and the signal intensity of MRI is the solutions of several concentrations of Gd-DTPA was measured. The results were; 1. In using a low magnetic field apparatus, signal intensity linearly correlated with the concentration of Gd-DTPA between 0 and 2.0 mumol/g under saturation recovery sequences (flip angle was 60 degrees or 90 degrees). Using a high magnetic field apparatus, signal intensity linearly correlated with the concentration of Gd-DTPA between 0 and 2.0 or 3.0 mumol/g under spin echo or gradient-echo sequences. 2. Gd-DTPA concentration of the renal cortex ranged from 0.132 to 0.152 mumol/g tissue at 5 min after intravenous injection of Gd-DTPA 0.05 mmol/kg body weight in 7 patients with adrenal tumor or renal cell cancer, and 1 patient with both urinary bladder cancer and prostatic cancer. Seven of them showed normal renal function and the other had renal insufficiency (GFR 25 ml/min/1.48 m2). Gd-DTPA concentrations of renal medulla and renal cell cancer tissue were 0.123 and 0.108 mumol/g tissue, respectively, at 5 min after intravenous injection of Gd-DTPA 0.05 mmol/kg body weight. These results suggest that the signal intensity of renal cortex, renal medulla, and renal cell cancer tissue may linearly correlate with Gd-DTPA concentration of tissues at 5 min after intravenous injection of Gd-DTPA 0.5 mmol/kg body weight.

Adult↗
PubMed1993

[Experimental reconstruction of the urinary bladder using atelocollagen sponge].

An experimental reconstruction of the urinary bladder using specially designed bovine cross linked atelocollagen sponge was performed after partial cystectomy in 12 male rabbits. Bladder reconstruction with hybrid type biomaterials using both atelocollage sponge and cultured autologous cells were performed in 5 rabbits (the 1st group). The autologous cells were collected from the mucosa and muscular layer of the urinary bladder and were seeded on the atelocollagen sponge before reconstruction. Reconstruction using atelocollagen sponge without autologous cells were performed in the other 7 rabbits (the 2nd group). In 9 of these 12 rabbits, atelocollagen sponge was successfully implanted in the native urinary bladder. Histopathological findings revealed that seeded autologous cells and growth of surrounding host cells could be seen similarly in the atelocollagen sponge but these could not be differentiated by these could not be differentiated by routine histopathological techniques. In conclusion, these results showed a possibility of construction of artificial urinary tract using both atelocollagen sponge and autologous cells.

Animals↗
PubMed1975

[Gonadotropin of chorioepithelioma -- its heterogeneity -- (author's transl)].

Crude gonadotropins extracted from the urine of patients with chorioepithelioma (choriocarcinoma) by Bradbury method was purified by a combination of Sephadex gel filtration, CM-C and DEAE-C chromatography. Two biologically active fractions (fraction t-hCG-A and fraction t-hCG-C) were obtained. The former cross-reacted with anti-hCG sera and the latter cross-reacted with anti-hCFSH sera in experiments with Ouchterlony immunodiffusion and immunoelectrophoresis. But the hCG or hCFSH from normal pregnancy and t-hCG-C from choriocarcinoma were different in their potency of being adsorbed on ion-exchange cellulose respectively. T-hCG-C was poorer in aspartic acid and glycine, and richer in serine, threonine and tyrosin to which carbohydrates bind than hCFSH from normal pregnancy. These two fractions contained high concentrations of carbohydrates, especially hexose and sialic acid, less concentration of hexosamine, compared to those of hCG and hCFSH obtained from urine of normal pregnant women. Sera of normal pregnant women in the first and third trimester and those of patients with chorionic neoplasias were gel filtrated on a Sephadex G-100 upward flow column in the same conditions. Biological activity of hCG in sera of normal pregnant women was recognized in 2-3 peaks on the gel filtration, and the molecular weights of those were considered to be about 25,000-40,000. In case of sera of chorionic neoplasias, however, it was admitted as multi-peaks (the molecular weights: about 10,000-70,000). It might be one of the features of chorionic neoplasias that the biological activity was found even in fractions of molecular weight 10,000 on the gel filtration, and to pay attention to this phenomenon might be a useful sign for the diagnosis or the management of patients with chorionic neoplasias. As a conclusion, all the above findings suggest that the molecular structure of t-hCG-A and t-hCG-C from choriocarcinoma differes from that of hCG and hCFSH from normal pregnancy respectively.

Amino Acids↗
PubMedDate not supplied

Differential diagnosis of solid renal tumors by MRI. Comparison of in situ relaxation times measured with a 0.1 T imager and histological findings.

The T1 and T2 of kidneys in 5 normal volunteers and 27 patients with renal tumors were evaluated preoperatively using a magnetic resonance imager with a 0.1 T resistive electromagnet, and the T1 and T2 of the renal tumors were compared with the pathological findings: (i) the T1 and T2 of the normal renal medulla were longer than those of the normal renal cortex and psoas muscle; (ii) both T1 and T2 of renal pelvic cancer (RPC) were shorter than those of renal cell cancer (RCC, p < 0.05); (iii) the T2 values of RPC were significantly shorter than those of renal medulla (p < 0.01); (iv) the T1 and T2 of the normal renal cortex of subjects < 50 years old were shorter than those of subjects > or = 50 years old (p < 0.05 and p < 0.01, respectively). These findings suggest that preoperative evaluation of the pathological findings of renal tumors may be possible by calculating the relaxation time using MRI under low magnetic field.

Adult↗