HSB-2 is a cell line originated from human T-cell leukemia and its membrane antigen is recognized by anti-HNK-1, a monoclonal antibody against human natural killer (NK) cells. Enzyme linked immunosorbent assay (ELISA) revealed that not only anti-HNK-1 but also rabbit polyclonal and human monoclonal anti-myelin-associated glycoprotein (MAG) antibodies were absorbed by cell homogenate of HSB-2. Molt-4 and K-562 cell lines from human T-cell leukemia and human erythroleukemia did not absorb anti-HNK-1 and the anti-MAG antibodies. On the immunoblot of HSB-2 cell homogenate, two major molecules were stained by anti-HNK-1 and anti-MAG antibodies, while Molt-4 and K-562 were not stained by these antibodies. Comparing molecular weights of the two major molecules with those of MAG and derivative of MAG (dMAG), one is larger than MAG and the other is smaller than dMAG. The molecular weights of the two molecules were approximately 130 kDa and 80 kDa. The shared antigen in HSB-2 is not identical with MAG but a part of the molecule in the cells may be shared with a part of MAG molecule.

Animals↗

PubMedDate not supplied

Computed tomographic findings of progressive supranuclear palsy compared with Parkinson's disease.

We investigated computed tomographic (CT) films of 4 pathologically documented cases of progressive supranuclear palsy (PSP) in which the clinical presentations were atypical and compared the findings with those of 15 patients with Parkinson's disease (PD). Dilatation of the third ventricle, atrophy of the midbrain tegmentum, and enlargement of the interpeduncular cistern toward the aqueduct were found to be the characteristic findings in PSP. Thus, radiological findings can be useful when the differential diagnosis between PSP and PD is clinically difficult.

Aged↗

PubMedDate not supplied

Treatment of neurological complications in isolated ACTH deficiency by glucocorticoid replacement.

The neurophysiological complications in ACTH deficiency have not been well documented. In this paper, we present a patient with isolated ACTH deficiency who developed various neurological signs. The neurophysiological abnormalities, including slow wave activity on electroencephalogram, delayed conduction velocity of the peripheral nerves and low amplitude of muscle action potentials, were improved by replacement of glucocorticoid. These findings suggested that glucocorticoid is directly involved in the function of the peripheral and central nervous systems.

Adrenocorticotropic Hormone↗