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Biomedical subjects

T Miyake

Publications and source records attributed to T Miyake.

At least 289 records · Page 16Linked to original sources
PubMed1988

17-beta-estradiol treatment maintains differentiative potential of virgin mouse mammary gland in culture and its responsiveness to insulin.

Synthesis of the milk proteins, casein and alpha-lactalbumin was not induced in cultured mammary explants from C3H/HeN castrated virgin mice in the presence of lactogenic hormones such as insulin, cortisol and PRL. Replacement therapy with 17-beta-estradiol to the castrated mice completely restored the differentiative potentials of mammary explants, inducing synthesis of the two milk proteins. [3H]thymidine incorporation into DNA synthesized in cultured mammary explants was also decreased by castration to less than 50% (P less than 0.001) of that obtained in intact mice, and 17-beta-estradiol treatment to castrated animals restored DNA synthesis to 90% of the intact level. The addition of insulin to culture medium significantly (P less than 0.001) enhanced [3H]thymidine incorporation into DNA in cultured mammary explants from both intact and 17-beta-estradiol-treated castrated mice but not from castrated animals. Insulin binding sites (1710 +/- 260 sites/cell) to mammary epithelial cells from castrated mice were significantly (P less than 0.05) lower than those from both intact (2870 +/- 300 sites/cell) and 17-beta-estradiol-treated castrated animals (2860 +/- 190 sites/cell). The present findings suggest that 17-beta-estradiol maintains growth and differentiative responses of mammary epithelial cells to insulin, which may be through preserving the number of insulin binding sites in the cells.

Animals↗
PubMed1988

A case of liver cirrhosis with large portacaval shunt from the superior mesenteric vein: normalization of reversed portal blood flow with medical treatment.

A case of liver cirrhosis with large portacaval shunt from the superior mesenteric vein is reported. The portal blood flow was diagnosed as reversed on admission, but normalized with treatment. Blood flow was measured noninvasively and repeatedly using an ultrasonic Doppler duplex system. The reversed portal flow led to the superior mesenteric vein, right (and some left) internal inguinal vein, and inferior vena cava. This rare collateral pathway was clearly demonstrated by percutaneous transhepatic portalgraphy. A link between shunt and an appendectomy carried out 22 yr ago is considered, and the possibility of the temporarily reversed portal blood flow on admission being due to deteriorated hepatic hemodynamic conditions is discussed.

Humans↗
PubMed1987

Immunohistochemical localization of a new thiamine diphosphate-binding protein in the rat nervous system.

The neurophysiological roles of thiamine-binding proteins have lately attracted considerable attention. We purified a new thiamine diphosphate-binding protein (ThDP-BP) prepared from rat livers and produced an antiserum against it. We examined the immunohistochemical distribution of ThDP-BP in the rat nervous system by the avidin-biotin complex technique. Immunoreactivity for ThDP-BP was found in several groups of neurons, and other neuronal tissues including glial cells, ependymal cells and Schwann cells. In neurons, the cytoplasm and processes were stained, but the nucleus was scarcely stained. In glial cells, the nucleus was stained, but the cytoplasm was not stained.

Animals↗
PubMed1987

Recombinant murine granulocyte-macrophage (GM) colony-stimulating factor supports formation of GM and multipotential blast cell colonies in culture: comparison with the effects of interleukin-3.

We studied the effects of murine recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) on murine hemopoiesis in methylcellulose culture. The GM-CSF was purified from cultures of Saccharomyces cerevisiae transfected with a cloned murine GM-CSF cDNA. In cultures of spleen cells from normal mice, only granulocyte-macrophage (GM) colonies were supported by GM-CSF. Blast cell colonies were the predominant type in cultures of spleen cells from 5-fluorouracil (5-FU)-treated mice. Dose-response studies revealed that maximal GM and blast cell colony formation is achieved with 100 U/ml GM-CSF. Blast cell colonies revealed variable but high replating efficiencies, and the secondary colonies included multilineage colonies. Serial replating of washed blast cell colonies in cultures with GM-CSF provided evidence for the direct effects of GM-CSF on the proliferation of multipotential blast cells. A combination of GM-CSF and interleukin-3 (IL-3) did not increase the number of blast cell colonies over the level supported by IL-3. This observation indicates that the progenitors for blast cell colonies that responded to GM-CSF are a subpopulation of multipotential progenitors that are supported by IL-3. Cytological studies of colonies derived from GM-CSF and/or IL-3 suggest that the eosinophilopoietic ability of murine GM-CSF is less than that of IL-3.

Animals↗
PubMed1987

Production of virus-like particles by the transposable genetic element, copia, of Drosophila melanogaster.

Using the calcium-phosphate procedure the plasmid cDm2055, which carries the copia transposable element of Drosophila melanogaster, was co-transfected with the Neor-carrying plasmid pSVCneo-1 into the D. hydei cell line KUN-DH-33 which was free of copia. The Neor transfectants stably carried both plasmids as tandem oligomers integrated in the chromosome and virus-like particles (VLP) were produced specifically in the transfectants that received the copia plasmid. The particles were quite similar in various aspects such as size, morphology, density, RNA content and molecular weight of the major protein component, to retrovirus-like particles (RVLP) that spontaneously appear in cultured cells of D. melanogaster: the reverse transcriptase activity however seemed to be low compared to that of the D. melanogaster RVLP. This finding demonstrates that the retrovirus-like particles (RVLP) in Drosophila cultured cells are produced by the transposable genetic element copia resident in the host chromosomes.

Animals↗
PubMed1987

A new method of lectin histochemistry for the study of brain angiogenesis. Lectin angiography.

In an attempt to analyse the kinetics of angiogenesis in the brain, we developed a new lectin-histochemical staining technique for identifying the vasculature. Three horseradish-peroxidase-conjugated lectins, i.e., Griffonia simplicifolia agglutinin 1 (GS1), Ricinus communis agglutinin 1 (RCA1) and soybean agglutinin (SBA), selectively stained vascular walls in brain-tissue sections. When these lectins were injected into the circulation of ether-anesthetized animals via the pulsating left ventricle, they bound specifically to the inner surface of endothelial cells and revealed the three-dimensional architecture of the vascular network within thick tissue preparations. When this technique, referred to a lectin angiography, was combined with 5-bromo-2-deoxyuridine (BudR) immunohistochemistry, proliferating capillary cells could be easily identified in three-dimensional structures of the developing vasculature. Because of its simplicity and wide applicability, lectin angiography should be useful for analysing the kinetics of angiogenesis in developmental, regenerative, and pathological conditions in various tissues and organs.

Aging↗
PubMed1987

Small bowel pseudo-obstruction in Kawasaki disease.

Between June 1977 and May 1986, 310 patients with Kawasaki disease were admitted to Shizuoka Children's Hospital. Among these patients, seven showed the symptoms of intestinal paralysis during the acute stage. We present three of these cases who showed typical intestinal pseudo-obstruction radiologically, and discuss the treatment of this type of intestinal involvement.

Child, Preschool↗
PubMed1987

Cell lines from imaginal discs of Drosophila melanogaster.

New cell lines, designated as ML-DmD1-10, were established from dissociated imaginal discs of Drosophila melanogaster. The culture medium was prepared by mixing in a 1:1 ratio Cross and Sang's M3(BF) medium, supplemented with 10% heat inactivated fetal bovine serum (FBS), with the supernatant of a primary embryonic cell culture made in the M3(BF) medium and supplementing this mixture with insulin. One cell line was established in the medium containing larval hemolymph instead of the primary culture supernatant, and another was established in fresh M3(BF) medium supplemented with insulin and FBS. In these mediums, imaginal disc cells first formed aggregates and cellular vesicles within a few weeks followed by the proliferation of thin-layered cells around them after about 1 mo. Ten cell lines have so far been established from two kinds of imaginal discs and disc mixtures. The ploidy of these cell lines was predominantly diploid. Population doubling time was about 50 to 70 h at 3 to 10 mo. after initiation of the culture. When the cell aggregates formed in vitro were implanted in metamorphosing larvae, they differentiated at high frequency into adult cuticular structures in the early phase of the primary culture. This differentiation of aggregates was also observed, though at low frequency, in a culture maintained by dilution-transfer for 6 to 15 mo. in vitro.

Animals↗
PubMed1987

Immunoglobulin-producing cells in secretory immune system in patients with selective IgA deficiency.

This study evaluated the class distribution and J chain-positivity of immunoglobulin-producing cells (Ig-PCs) in gastrointestinal mucosa and salivary glands of Japanese patients with selective IgA deficiency. The proportional patterns of gland-associated Ig-PCs showed an increase in not only IgM cells but also IgG cells, most of which were J chain-positive. This may represent a maturation arrest at B cell differentiation, rather than a compensatory phenomenon. No patients showed an increase of IgD cells in salivary glands. This behavior of IgD cells in Japanese patients may reflect associated diseases or ethnic difference in B cell differentiation.

Adolescent↗
PubMed1987

Gastric plasmacytoma with cylindrical crystalline inclusions.

An 81 year-old female case of extramedullary plasmacytoma of the stomach is reported. Immunohistochemically, the tumor cells contained IgM-lambda immunoglobulin. Electron micrographs of the tumor cells showed centrally located nuclei with nuclear inclusions and cytoplasm filled with crystalline inclusions in the rough endoplasmic reticulum. IgM-lambda producing gastric plasmacytoma with cylindrical inclusions is rare.

Aged↗