Biomedical subjects
Publications and source records attributed to T Mitsuoka.
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Chromogenic Limulus amoebocyte lysate (LAL) tests were evaluated for measuring endotoxic activity in faeces of mice from different microbiological environments. Faeces of BALB/c mice from germ-free (GF), specific-pathogen-free (SPF) and conventional (CV) colonies had no inhibitory activity on the test reaction. GF and SPF faeces had heat-labile non-specific amidase activity which gave false-positive reaction, while CV did not. GF faeces had a heat-stable non-endotoxic effect on the LAL system. Average endotoxin levels in faeces of GF, SPF and CV mice were 0.2, 0.8 and 17 to 24 micrograms/g wet weight, respectively. Results suggest that the microbiological status of mice influences not only the faecal endotoxin level but also the specificity of the test reaction.
SUMMARY: To evaluate associated cartilaginous damage with acute isolated posterior cruciate ligament (PCL) injury without other concomitant ligamentous injury, arthroscopic evaluations were performed on 61 consecutive patients. Meniscal tear was found in 17 cases (28%). Of these, 3 had medial meniscal tear, 11 had lateral meniscal tear, and 3 had both medial and lateral meniscal tears. Longitudinal tears of anterior segment in lateral menisci were the most common (10 cases). Thirty-two patients (52%) had articular cartilage injury. Of these, 7 had damage greater than one half of the thickness of the articular cartilage, and 3 had erosion that extended to the subchondral bone. The most frequently injured location was the medial femoral condyle (19 cases, 31%). Significant cartilaginous injury could be combined in acute isolated PCL injury. Therefore, it is unreasonable to manage every acute isolated PCL-injured knee using a single treatment modality. Concomitant meniscal and articular cartilaginous lesions should be evaluated when treatment for acute PCL injury is planned.
Differences in the features of osteochondritis dissecans (OCD) affecting the lateral and medial femoral condyles were investigated in 13 patients (14 knees) treated from 1991 to 1994. OCD affected the lateral femoral condyle in 6 knees (lateral group) and the medial condyle in 8 knees (medial group). The lateral group was younger (mean age, 14 v. 20 years). The radiological stage (Brückl) of the lateral group was stage 2 in 3 knees and stage 3 in 3. The lateral menisci were all discoid and the condylar articular surface of the lesions was normal in two knees, softened in 3 and detached in 1. The medial group comprised 1 knee in stage 2, 1 in stage 3, 1 in stage 4, and 5 in stage 5. The OCD lesion showed softening in 2 knees and detachment in 6. Repetitive abnormal stress on weaker osteochondral structures in the growing period produced by a discoid meniscus during growth may cause OCD of the lateral femoral condyle.
Eighty-six chronic anterior cruciate ligament (ACL)-injured patients were quantitatively measured for anterior knee stability preoperatively and at 2 years or later (mean, 30 months) postoperatively to examine the influence of preoperative knee laxity on the postoperative knee stability restored by the ACL reconstruction using multiplied hamstring tendon graft. The patients were divided into 3 groups according to the preoperative injured minus normal anterior laxity difference (AL-D) (group I [n = 27] <5 mm, group II [n = 48] 5 to 9 mm, group III [n = 11] >10 mm). The 3 groups were comparable in gender, age, meniscal status, graft excursion measured during operation, time from injury to operation, and activity level. The postoperative AL-D in group I was 0.8 +/- 1. 7 mm, that in group II was 1.1 +/- 1.6 mm, and in group III was 1.5 +/- 1.4 mm. There were no significant statistical differences among these 3 groups. With our surgical technique including postoperative rehabilitation, patients with severe instability could be treated successfully without needing any additional procedures such as extra-articular augmentation or specially designed postoperative rehabilitation programs.
We investigated the direct effects verapamil, which was infused into the A-V node artery at two doses of 0.01 and 0.1 microgram/kg/min on the A-V conduction and the A-V junctional automaticity in the dog with experimentally induced A-V junctional rhythm. Verapamil significantly prolonged the A-H interval, but not the H-V interval, in the His bundle electrogram during atrial pacing at a rate of 100 beats/min. Effective and functional refractory periods of the A-V node also were prolonged significantly by verapamil. On the other hand, verapamil showed less propensity to decrease the spontaneous A-V junctional rate and to prolong the A-V junctional recovery time after atrial overdrive at a rate of 100 beats/min for 60 s. These results indicate that suppressive effects of verapamil on the A-V junctional automaticity are much weaker when compared with that on the A-V conduction.