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Biomedical subjects

T Mitsui

Publications and source records attributed to T Mitsui.

At least 253 records · Page 14Linked to original sources
PubMed1993

Genotype dependence of hepatitis C virus antibodies detectable by the first-generation enzyme-linked immunosorbent assay with C100-3 protein.

Hepatitis C virus (HCV) samples in 155 sera, from patients with chronic non-A, non-B liver disease and blood donors, were grouped into four genotypes (I, II, III, and IV) by amplification of core-gene sequences by polymerase chain reaction with type-specific primers. HCV genotypes were compared with various HCV-associated antibodies detectable by the first-generation ELISA (ELISA-1) with C100-3 protein and a second-generation immunoblot assay with four recombinant HCV proteins. Antibodies to C100-3 protein and those to its subsequence (5-1-1) were detected in 13 (93%) and 12 (86%), respectively, of 14 sera with genotype I HCV; 56 (79%) and 58 (82%) of 71 sera with genotype II; 13 (34%) and 6 (16%) of 38 sera with genotype III; and 11 (34%) and 4 (13%) of 32 sera with genotype IV. Amino acid sequences of C100-3 of genotype I HCV are conserved by approximately 90% in genotype II, but only by approximately 75% in genotypes III and IV. The sensitivity of ELISA-1, therefore, would be influenced by heterogeneity in C100-3 sequences of different genotypes.

Antigens, Viral↗
PubMed1993

Autonomic hyperreflexia in pure progressive autonomic failure: a case report.

A 60-year-old woman suffered from recurrent episodes of fever, hypertension, facial flushing, vomiting, stridor, slowly progressive symptoms of hypohidrosis, and orthostatic hypotension. The episodes were synchronous with elevated catecholamine concentration in plasma and urine. This is an example of paroxysmal autonomic hyperreflexia in a setting of pure progressive autonomic failure.

Adult↗
PubMed1993

hlyIA-like sequence present in a genetic locus with low homology to appA in Actinobacillus pleuropneumoniae serotype 10.

Using the appA gene encoding Actinobacillus pleuropneumoniae-RTX-toxin II (ApxII) as a probe, Southern hybridization demonstrated the presence of appA-sequence in all 12 serotypes except serotype 10. Serotype 10 strains had a genetic locus with low homology to appA (tentatively designated Lha). DNA sequence analysis revealed that Lha contained a part of hlyIA gene encoding a 105-kDa hemolysin ApxI. Southern hybridization demonstrated the presence of Lha (hlyIA) sequence not only in serotype 10 but also in serotypes 1, 5a, 5b, 9, and 11, which had strong hemolytic activity with sheep red blood cells and were highly virulent for mice. These results suggest that ApxI may be associated with the virulence phenotype of the organism.

Actinobacillus Infections↗
PubMed1993

Distribution of vinculin in the duodenum of chick embryos: immunohistochemical studies at the light and electron microscopic level.

Studies on the localization of vinculin and actin in the embryonic chick duodenum between the 9th and 13th days of incubation were performed by light and electron microscopy. In the light microscopic studies, vinculin and actin were localized by immuno-fluorescent double staining with anti-vinculin and rhodamine phalloidin for actin. In the electron microscopic studies, the immunocytochemical distribution of vinculin was examined on Lowicryl K4M thin sections with anti-vinculin. Vinculin was prominent near or adjacent to the apical inner membrane of epithelial cells of the previllous ridges in all stages, often located near cell junctions including tight and adherent junctions and the top of microvilli, and weakly in the cytoplasm. Our findings suggested the possible interaction between actin and vinculin in differentiation on the apical side of epithelial cells including the formation of microvilli or terminal webs.

Actins↗
PubMed1993

[Right thoracotomy to approach mitral or tricuspid valve in reoperation].

Four patients underwent mitral valve re-replacement, and required tricuspid annuloplasty in two, through a right thoracotomy. There was no major postoperative morbidity and recovery was full and uneventful. The indications for selection of this approach were isolated mitral valve disease or combined tricuspid and mitral valve disease, severe adhesion between heart and sternum on CT scan, intact aortic valve and no severe reduction of respiratory function. We recommend right thoracotomy to approach atrioventricular valves in selected situations in patients for reoperations.

Aged↗
PubMed1993

[Two cases of coarctation complex associated with congenital esophageal atresia (Gross type C)].

Two cases of coarctation complex (CoA + VSD + PDA) associated with congenital esophageal atresia (Gross type C) were successfully treated with esophageal and two-staged cardiac surgery. The perioperative management of the two cases is discussed with special reference to circulatory, respiratory and nutritional problems. Case 1; A newborn was diagnosed as esophageal and cardiac anomalies and gastrostomy was performed followed by end to end anastomosis of the esophagus under receiving Prostaglandin E1 infusion. Although subclavian flap angioplasty (SFA) and pulmonary artery banding (PAB) were performed 1 month later, she had recurrent respiratory complications and malnutrition postoperatively due to bronchomalacia and gastroesophageal reflux (GER). Case 2; A newborn was diagnosed as esophageal atresia and underwent corrective surgery of it on the 1st day of life. After esophageal surgery congestive heart failure (CHF) developed progressively and CoA complex was evident by echocardiography 5 days later. SFA and PAB were performed 26th day of life. In spite of the palliative surgery for cardiac lesions, both patients had growth failure because of CHF, respiratory complications and malnutrition. As soon as the circulatory and respiratory condition improved, corrective surgery was performed and resulted in full recovery.

Aortic Coarctation↗
PubMed1993

[A case report of surgically treated tracheobrachiocephalic artery fistula following tracheostomy].

The patient was a 17 year-old-boy with mental retardation and cerebral palsy, who underwent tracheostomy because of tracheobronchomalacia and tongue swallowing three months ago. After minimal tracheostomy bleeding was noted for a few days, massive hemorrhage occurred suddenly. The patient was severely hypotensive. The cuff on the tracheostomy tube was hyperinflated, which stopped the bleeding temporarily. Through a median sternotomy and collar incision, a pin hole-sized defect in the medial surface of the brachiocephalic artery at the tracheal balloon was repaired by suture. The tracheal defect allowed visualization of the tracheal cannula balloon. The pectoralis major muscle flap was interposed between the tracheal defect and the artery. The tracheal defect was packed with muscle. Seven days after the operation, the second operation was done for recurrent bleeding. The defective segment of brachiocephalic artery was resected and the ends were oversewn with monofilament sutures. Continuous irrigation with povidone-iodine solution was continued for purulent mediastinitis. We believe that interruption of blood flow of the brachiocephalic artery, interposition and packing of muscle flap over the tracheal defect and continuous irrigation for infection were effective treatment of choice.

Adolescent↗
PubMed1993

[Mitral valve re-replacement for a patient with multiple myeloma].

Mitral valve re-replacement was successfully undertaken in a 70-year-old female patient with primary tissue failure of mitral bioprosthesis and multiple myeloma (MM) through right lateral thoracotomy with minimum dissection. Considering the post-operative anti-coagulant therapy, bioprosthesis was chosen in this complicated case. Patients with MM usually show a bleeding tendency associated with abnormally elevated IgG which covers the surface of coagulation factors. Safety range of serum IgG concentration in the patients with MM who require open heart surgery, have not defined yet. Excessive serum IgG leads the coagulation disorder and organ perfusion problem because of increase of blood viscosity. Those problems become serious when the serum IgG concentration exceed 2,500 mg/dl. (1) Only if serum IgG level below 2,5000 mg/dl, valve replacement will be able to perform safely with minimum dissection through the thoracotomy for patients with MM. (2) Bioprosthesis might be optionally chosen in such case (3). In case of serum IgG level over 2,500 mg/dl, preoperative plasma ex-change should be recommended.

Aged↗
PubMed1992

Purification and characterization of smooth muscle myosin-associated phosphatase from chicken gizzards.

Myosin light chain phosphatase associated with smooth muscle myosin (MAPP) was isolated from chicken gizzard. The MAPP was tightly associated with myosin and was not dissociated from myosin under the physiological ionic conditions. The phosphatase was dissociated from myosin in the presence of high MgCl2, i.e. 80 mM MgCl2. The binding site of the enzyme on the myosin molecule was the subfragment-2 region, since the enzyme did bind to the myosin rod and heavy meromyosin but not to the subfragment-1 affinity column. MAPP was purified with a heparin-Sepharose 6B column, and two activity peaks were obtained, i.e. MAPP I and MAPP II. The major activity peak, MAPP I, was further purified to homogeneity by thiophosphorylated myosin light chain-Sepharose 4B column chromatography. MAPP I was a tetramer composed of four 34-kDa subunits. The enzyme preferentially dephosphorylated the beta-subunit of phosphorylase kinase and was strongly inhibited by the heat- and acid-stable protein phosphatase inhibitor-1, whereas it was partially inhibited by the inhibitor-2. The IC50 (concentration of inhibitor giving 50% inhibition) value for the inhibition of the enzyme by okadaic acid was 70 nM which was about eight times higher than skeletal muscle type-1 and 390 times higher than type-2 protein phosphatase. These results demonstrate that the MAPP I is a type-1-like protein phosphatase, although the properties are not the same as type-I phosphatase. The properties of the myosin-associated phosphatase were distinct from the phosphatases reported previously, although some properties were similar to smooth muscle phosphatase-IV. Therefore, it is concluded that MAPP I is a novel smooth muscle protein phosphatase. Since it strongly associated with smooth muscle myosin, it is likely that MAPP I is responsible for the dephosphorylation of smooth muscle myosin in situ.

Adenosine Triphosphate↗
PubMed1992

Isolation and primary structure of a novel pheromonotropic neuropeptide structurally related to leucopyrokinin from the armyworm larvae, Pseudaletia separata.

A novel pheromonotropic neuropeptide has been isolated from a head extract of the armyworm larvae, Pseudaletia separata, by a seven step purification procedure using an in vivo assay with decapitated female moths of Bombyx mori. Amino acid sequence analysis and comparison with synthetic peptides established the primary structure of the peptide, termed Pseudaletia pheromonotropin (Pss PT), as H-Lys-Leu-Ser-Tyr-Asp-Asp-Lys-Val-Phe-Glu-Asn-Val-Glu-Phe-Thr-Pro-Arg-Le u-NH2. Pss PT is structurally related to leucopyrokinin, an insect myotropic neuropeptide, and possesses the C-terminal pentapeptide, Phe-Thr-Pro-Arg-Leu-NH2, responsible for the biological activity.

Amino Acid Sequence↗
PubMed1992

Antibodies against synthetic oligopeptides deduced from the putative core gene for the diagnosis of hepatitis virus infection.

Immunoassays were developed to detect antibodies against oligopeptides deduced from the putative core gene of hepatitis C virus, and their performances were compared with that of the commercial immunoassay for antibodies against the product of nonstructural regions of hepatitis C virus (anti-C100-3). A 19-mer oligopeptide (CP10) and a 36-mer oligopeptide (CP9) were chemically synthesized, which represented hydrophilic regions of the product of the hepatitis C virus core gene. They were used to capture corresponding antibodies, anti-CP10 and anti-CP9, by enzyme-linked immunosorbent assay in sera from patients with acute or chronic non-A, non-B liver disease and in blood donations. At the onset of acute non-A, non-B hepatitis, anti-CP10 was detected in 15 of 20 patients (75%), and anti-CP9 was detected in 14 patients (70%). This was more frequent than anti-C100-3, which was found in only 9 patients (45%). In 186 patients with chronic non-A, non-B liver disease, anti-CP9, anti-CP10 or both were detected in 170 patients (91%). This was more frequent than anti-C100-3, which was found in 138 patients (74%). Blood with anti-CP10 as the single serological marker for hepatitis C virus infection transmitted non-A, non-B hepatitis by needlestick exposure. In sera from 558 apparently healthy blood donors, anti-CP10 was detected in 55 donors (9.9%), anti-CP9 was detected in 26 donors (4.7%) and anti-C100-3 was detected in 7 donors (1.3%).(ABSTRACT TRUNCATED AT 250 WORDS)

Amino Acid Sequence↗
PubMed1992

[A case of malignant rheumatoid arthritis with corticosteroid-reactive subacute myelopathy and multiple peripheral neuropathy].

We reported a 64-year-old man who had malignant rheumatoid arthritis (MRA) and developed subacute myelopathy and peripheral neuropathy. He had suffered from seropositive rheumatoid arthritis for 4 years, and developed weakness of four limbs, dysuria and constipation two months before the admission. Neurological examination revealed the diffuse muscle wasting and weakness in four limbs. Deep tendon reflexes were hyperactive in four limbs, but not in jaw jerk. Babinski sign was positive bilaterally. Deep sensation was decreased in four limbs and superficial sensation was decreased below the neck. Dysuria and constipation were noted, but anal and bulbocavernosus reflexes were present. On laboratory examination, RF and RAHA increased markedly. Serum complements decreased and immune complexes were positive. Nerve conduction study demonstrated multiple entrapment neuropathy in addition to mononeuritis multiplex. Histological examination of the biopsied sural nerve disclosed the obliterating endarteritis in the epineurium, and marked decrease in number of myelinated fibers. No compressive lesions were seen in the spinal canal by spine X-ray and MRI. Assuming that inflammatory process induced cervical myelopathy, corticosteroid therapy (predonisolone 60 mg/day) was administered and alleviated neurological symptoms, in accordance with the improvement of serological abnormalities. Therefore, an inflammatory process associated with MRA was supposed to damage the spinal cord as well as peripheral nerves in the present case.

Acute Disease↗
PubMed1992

Hepatitis C virus infection in medical personnel after needlestick accident.

Hepatitis C virus infections in medical personnel after needlestick accidents have been documented generally by detection of seroconversion to a hepatitis C virus nonstructural region antigen, c100-3 (a marker of infection). We tested for hepatitis C virus core-derived antibodies and genomic RNA in addition to c100-3 antibody in 159 cases of needlestick exposure that did not involve patients positive for HBsAg. Of these we found 68 cases with index patients positive for both hepatitis C virus RNA and antibodies and members negative for antibodies to HCV core or c100-3 before the needlestick accidents. Seven of these medical personnel became infected with hepatitis C virus after the accidents. Their hepatitis was generally subclinical or self-limited and transient, except for one patient in whom liver enzyme elevation persisted along with the antibodies. In our study, the risk of hepatitis C virus transmission from a single needlestick accident with hepatitis C virus RNA-positive blood was 10%, considerably higher than the 4% estimated in a previous study. We found that donor blood with antibody to an hepatitis C virus core-derived peptide with enzyme-linked immunosorbent assay optical densities greater than 2.0 carried a significant risk of transmitting hepatitis C virus to needlestick victims. No hepatitis C virus seroconversions occurred in medical personnel exposed to hepatitis C virus antibody-negative or hepatitis C virus RNA-negative blood; however, one such exposure resulted in a very mild non-A, non-B, non-C hepatitis.

Health Personnel↗
PubMed1992

[The usefulness of the emergency hepatobiliary scintigraphy to rule out acute cholecystitis--43 patients report].

We studied emergency hepatobiliary scintigraphy in the 43 patients to rule out acute cholecystitis. After injection of 185-222 MBq (5-6 mCi) of 99mTc-EHIDA or 99mTc-HIDA, serial static scintigraphic images were obtained up to 7 hours in maximum. Of 43 patients in this study, 20 had a normal scan and finally in all of them cholecystitis was ruled out. Of the 43 patients, 14 had an abnormal scan (nonvisualized gall bladder). In 10 of them the diagnosis of acute cholecystitis was confirmed after emergency cholecystectomy. The other 9 patients of 43 had an incomplete scan mainly due to liver dysfunction. Four of them had acute cholecystitis in the cholecystectomy. These results indicate that acute cholecystitis can be excluded by the findings of gall bladder visualization in hepatobiliary scintigram. We concluded that emergency hepatobiliary scintigraphy is very useful to rule out acute cholecystitis.

Acute Disease↗
PubMed1992

[A case report of poststernotomy mediastinitis treated with the pectoralis major muscle flap after protracted wound irrigation].

A 8-year-old boy developed mediastinitis after direct closure of atrial septal defect. Methicillin-resistant Staphylococcus aureus (MRSA) was detected from the wound. Intermittent wound irrigation with povidone-iodine solution and vancomycin solution was protracted for two months. After the remission of MRSA infection, implantation of pectoralis major muscle flap was performed. Without recurrence of infection the wound was healed completely. Although pectoralis major muscle flap is an effective method of choice for mediastinitis, an appropriate timing of enforcing this method should be investigated hereafter.

Child↗