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Biomedical subjects

T Minowa

Publications and source records attributed to T Minowa.

At least 73 records · Page 4Linked to original sources
PubMed1989

Crystallization of and preliminary crystallographic data for allosteric L-lactate dehydrogenase from Bifidobacterium longum.

L-Lactate dehydrogenase from Bifidobacterium longum aM101-2 was overexpressed in Escherichia coli and then purified. The enzyme was crystallized from a polyethylene glycol 6000 solution by the hanging drop vapor diffusion method. Crystals grown in the presence of NADH (type II), both NADH and oxamate (type III), and NADH, oxamate, and FBP (type IV) were analyzed. All three crystal forms belong to the orthorhombic system, space group P2(1)2(1)2. The cell dimensions of the type II crystals were a = 106.2 A, b = 131.6 A, and c = 63.8 A. Those of the type III and type IV crystals were a = 106.4 A, b = 131.4 A, and c = 63.8 A. The type III crystals diffract X-rays to beyond 2.5 A spacing. The type II and type III crystals were stable as to X-ray irradiation.

Allosteric Site↗
PubMed1984

[Evaluation of serum tissue polypeptide antigen (TPA) in patients with urogenital cancer].

The level of serum TPA was determined by radio-immunoassay in 19 healthy subjects and 90 patients with urogenital cancer. The normal level of serum TPA was 86 +/- 24 U/l, and the level of more than 134 U/l was determined positive. The positive rate of TPA was 38.9% in 90 patients, while that of CEA was 25.6%. In 19 patients with bladder tumor and 7 with testicular tumor, the positive rates of TPA were 52.6% and 71.4%, respectively, and the level of serum TPA was high in these positive patients. Considering the low positive rate of CEA, TPA may be a more useful marker than CEA in patients with bladder tumor and testicular tumor. Serial determinations of serum TPA and CEA showed the considerable variation of serum TPA compared with serum CEA and a temporary elevation of serum TPA following radical nephrectomy and retroperitoneal lymphadenectomy. However, the level of serum TPA fell significantly after the successful treatment in 8 patients (2 with renal cell cancer, 3 with bladder tumor, 1 with prostate cancer, 2 with testicular tumor) and rose sharply with recurrent or metastatic disease in 4 patients (2 with bladder tumor, 2 with testicular tumor). Although there was no correlation between the levels of serum TPA and serum PAP, the level of serum TPA tended to change in parallel with the level of serum AFP or HCG in 3 patients with testicular tumor.

Adenocarcinoma↗
PubMed1983

Evaluation of multidisciplinary treatment of bladder cancer, especially in chemoimmunotherapy (ADM and OK-432) as a consolidation therapy.

The relapse rate of bladder cancer (transitional cell Ca) is said to be about 45%-80% even after tumor resection. Multidisciplinary treatment was designed and studied to prevent such recurrence. This treatment was designed to have three steps: induction, consolidation, and maintenance therapy. Following surgical tumor removal, OK-432 and Adriamycin (ADM) were administered as consolidation therapy, followed by administration of PSK and carboquone (CQ) in small amounts as maintenance therapy continuously for about 3 years, and the course was observed. In both consolidation and maintenance groups various non-specific immunoparameters were superior in groups receiving combined immunotherapeutic agents. Thus, the use of immunotherapeutic agents in combination with chemotherapeutic agents was considered to be effective. The 3-year recurrence rate was only 8% in the multidisciplinary treatment group, while that in the non-multidisciplinary treatment group was 61%. This approach, especially with chemoimmunotherapy (ADM and OK-432) as a consolidation therapeutic mode, is therefore considered to be useful for the prevention of recurrence.

Adjuvants, Immunologic↗
PubMed1983

[Studies on human prostatic carcinoma cell lines: evaluation of acid phosphatase productivity and effects of sex hormones on the cells].

The established cell lines from human prostatic cancer, such as Duke 145, 8PC93, and 19PC93, were examined in terms of their producing activity of acid phosphatase (ACP) and sensitivity to sex hormones. The results obtained are summarized. 1. ACP producing activity ACP was estimated with phenyl phosphate as a substrate. Values of the materials from each of the cells extracted with 5% Triton X-100 were Duke 145 (6.1 u/mg), 8PC93 (40.6 u/mg), and 19PC93 (40.4 u/mg), respectively. Activities of ACP were prohibited by the presence of L-tartrate. Histochemistry of ACP was demonstrated by azo-dye staining procedure, revealing the positive reactions in the cytoplasms of 8PC93 and 19PC93 cells, but weak reaction in duke 145 cells. Disk polyacrylamide gel electrophoresis (D-PAGE) was employed for ACP analysis of the cell extracts with 5% Tryton X-100 treatment. Two main bands were observed near original point and at another point proposed as ACP-2. These ACP positive reactions on the gels were also inhibited by the presence of L-tartrate in staining solution. In the case of Duke 145 cell material, the intensity of the reaction was observed weak in those specific two bands. 2. Hormone effects to the cells The prostatic cancer cells were examined in terms of sensitivity to sex steroid hormones such as androsterone, progesterone, estrone, estradiol, and estriol, by a colony formation method. Fifty percent reduction in colony formation of the 8PC93 and 19PC93 cells was found at the concentration of ca. 1.5 micrograms/ml in the case using progesterone or estrone, or estradiol, while 50% reduction of the Duke 145 cells was observed at 5 micrograms/ml only in a case using progesterone.

Acid Phosphatase↗
PubMed1982

Effects of PGD2 and PGF2 alpha on longitudinal and circular muscles of guinea-pig isolated proximal colon.

The mechanical effects of PGD2 and PGF2 alpha on longitudinal and circular muscles of the guinea-pig isolated proximal colon were investigated. PGD2 and PGF2 alpha (1 nM - 10 microM) produced a dose-dependent contraction in longitudinal and circular muscles. The contractile action of PGD2 was more potent than that of PGF2 alpha in circular muscle and was less potent in longitudinal muscle. Contractions induced by PGD2 or PGF2 alpha (1 microM) were unaffected by atropine(1 microM) in both muscles, but tetrodotoxin(1 microM) slightly inhibited these contractions in longitudinal muscle. The results suggest that in longitudinal muscle PGD2 and PGF2 alpha have largely a direct action on the muscle cells and a partial neuronal action on the non-cholinergic intrinsic nerves, whereas in circular muscle these PGs have only a direct action on the muscle cells.

Acetylcholine↗