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T Miller

Publications and source records attributed to T Miller.

At least 145 records · Page 8Linked to original sources
PubMed1994

Independent and coordinate regulation of beta 1- and beta 2-adrenergic receptors in rat C6 glioma cells.

Rat C6 glioma cells have both beta 1- and beta 2-adrenergic receptors in approximately 7:3 ratio. When the cells were exposed to the beta-adrenergic agonist isoproterenol, there was a rapid sequestration of up to 50% of the surface receptor population over a 30-min period as measured by the loss of binding of the hydrophilic ligand [3H] CGP-12177 to intact cells. Using the beta 1-selective antagonist CGP 20712A to quantify the proportion of the two subtypes, it was found that although both beta 1 and beta 2 receptors were sequestered, the latter were sequestered initially twice as fast as the former. More prolonged agonist exposure led to a down-regulation of approximately 90% of the total receptor population by 6 h as measured by the loss of binding of the more hydrophobic ligand [125I]iodocyanopindolol to cell lysates. The two subtypes, however, underwent down-regulation with similar kinetics. Treatment of the cells with agents that raise cyclic AMP levels such as cholera toxin and forskolin resulted in a slower, but still coordinated down-regulation of both subtypes. Thus, there appears to be both independent and coordinate regulation of endogenous beta 1-and beta 2-adrenergic receptors in the same cell line.

Animals↗
PubMed1993

Provisionally unique autosomal recessive chondrodysplasia punctata syndrome.

Stippled epiphyses occur in several monogenic, teratogenic, or aneuploidy syndromes. We describe two sibs with a provisionally unique chondrodysplasia punctata syndrome, who have, in addition to stippled epiphyses, minor facial anomalies, short stature, and ocular colobomata. Inheritance of this condition is likely autosomal recessive.

Abnormalities, Multiple↗
PubMed1993

Cloning and sequence analysis of the spike gene from several feline coronaviruses.

The DNA sequence encoding the spike gene from the DF2 strain of Type II feline infectious peritonitis virus (FIPV), a temperature sensitive FIPV virus (TS-DF2) and an isolate of feline enteric coronavirus (FECV 1683) were determined. Comparison of the published WSU 1146 and DF2 FIPV S genes showed that the viruses shared a high degree of homology (99.6%). Likewise, the S gene of the virulent DF2 FIPV virus was closely conserved to that isolated from the vaccine virus strain, TS-DF2 FIPV. In contrast, the FECV S gene had numerous DNA and amino acid differences when compared to the virulent FIPV sequences. Sequence differences among the feline coronavirus isolates were localized to the amino-terminus region of the S gene.

Amino Acid Sequence↗
PubMed1993

The families of problem drug users: a study of 50 close relatives.

The research described in this paper resulted from a collaborative multi-centre study of the relatives of problem drug users involving six practitioners and researchers, in four centres within the south-west of England. The objective of this research was simply to interview 50 close relatives of identified problem drug users, with the identification occurring through clinics and self-help groups. Quantitative and qualitative results show that large numbers of these relatives reported many negative experiences. The partners of illicit drug users reported both more and differently patterned problematic behaviours than those of prescribed tranquillizer users. The partners of illicit users also reported different problems to those of the parents. Relatives reported many negative effects in terms of how they viewed the drug user, and how the experiences had affected their health. They also described various coping mechanisms, and the extent of the support which they had received. The results are discussed in terms of coping, and similarity with research into the families of problem drinkers.

Adaptation, Psychological↗
PubMed1993

Orbital pseudotumor. Fine-needle aspiration biopsy and response to therapy.

PURPOSE: To determine the diagnostic accuracy of fine-needle aspiration biopsy and study the natural history and therapeutic response of a group of patients with orbital pseudotumor. METHODS: Retrospective review of patients seen by one ocular oncologist for diagnosis and management of presumed orbital pseudotumor. RESULTS: Thirty-eight patients had fine-needle aspiration biopsy under computed tomographic (CT) guidance. No false-positive results were observed. In 10% of fine-needle aspiration biopsies, negative results were observed. In all patients in this series, lymphomas could be differentiated from pseudotumors. Flow cytometry or Southern blot analysis could be performed on these cytopathologic specimens and was useful in selected cases. Eighty-one patients were followed after various forms of treatment. Essentially complete remissions were observed in 49 of 81 patients. High-dose oral corticosteroids were effective in approximately 31% of patients. A poor steroid response was observed in patients with mass lesions and those with fibrotic disease. Surgical resection was effective in 76% of patients; most of these had relatively discrete mass lesions. Radiation was effective in approximately 64% of patients. Radiation was more effective in nonfibrotic lesions and in patients with erythema. This type of treatment was less effective in fibrotic lesions and in patients who had a longer interval between diagnosis and therapy. CONCLUSIONS: Fine-needle aspiration biopsy is useful in evaluating patients with presumed orbital pseudotumor. There is a high success rate in many patients with orbital pseudotumor, although patients with diffuse fibrotic disease have a poor prognosis.

Adolescent↗
PubMed1993

Death-induced changes to the nuclear matrix: the use of anti-nuclear matrix antibodies to study agents of apoptosis.

Using immunological techniques, we have shown for the first time that change in the nuclear matrix is an observable step in the cell death program. Immunometric assays detect soluble nuclear matrix proteins in the culture supernatants of cells killed by adriamycin, tumor necrosis factor and serum growth factor deprivation. Studies of cells killed by tumor necrosis factor and serum growth factor deprivation using dual immunofluorescent and 4'-6-diamidino-2-phenylindole (DAPI) staining detect many cells strongly positive for DNA that have little or no nuclear matrix protein. The staining pattern of cells killed by adriamycin is very different. There is good correspondence between the immunofluorescent and DAPI staining patterns indicating the presence of nuclear matrix proteins and DNA, although at a reduced ratio. It is concluded that the nuclear matrix changes are dependent on the agents that trigger cell death.

Apoptosis↗
PubMed1993

Supraventricular tachycardia: catheter ablation therapy in 72 patients.

Radiofrequency (RF) catheter ablation for recurrent supraventricular tachycardia (SVT) has been performed at the Charleston Area Medical Center since September 1991. Of the first 72 patients undergoing this procedure, a total of 76 tachycardias were treated and these included 41 accessory pathways, 21 junctionals, and 14 atrial tachycardias (atrial fibrillation, flutter, and intra-atrial reentry tachycardia). RF catheter ablation was successful in 67 of these patients (93%). This procedure is a highly effective treatment modality which should be considered in patients with recurrent symptomatic SVT.

Adult↗
PubMed1993

Erythropoietin therapy in renal failure.

It is indeed rare that a medication can do so much good and be associated with so few side effects and complications. The judicious use of Epo along with informed dosing practices will lead to an overwhelming favorable balance in the risk/benefit ratio of care. The move from a very specialized area of need into a more generic arena has done well for the development of drugs of this nature and may help stimulate further research in other "orphan" areas of drug need.

Amino Acid Sequence↗
PubMed1992

Lin et al. reply.

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Journal Article↗