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Biomedical subjects

T Miki

Publications and source records attributed to T Miki.

At least 955 records · Page 53Linked to original sources
PubMed1983

Combination chemotherapy including adriamycin for advanced transitional cell carcinoma of the urinary tract.

Thirty-three patients with advanced transitional cell carcinoma of the urinary tract (23 bladder cases, 8 ureter cases, and 2 renal pelvis cases) were treated by three-drug combination chemotherapy using two protocols (protocol I: Adriamycin 50 mg/m2, cyclophosphamide 500 mg/m2, and 5-fluorouracil 500 mg/m2, protocol II: Adriamycin 50 mg/m2, cyclophosphamide 500 mg/m2, and cis-platinum 50 mg/m2). Protocol I induced responses in three of 19 patients (16%), (1 complete response, 2 partial responses), and protocol II (I complete response, 4 partial responses) in five of 14 patients (36%). The overall response rate was 24%. The duration of response was relatively short (median duration 5.1 months). The combination therapy was relatively well tolerated except in three patients, including two mortalities. In our study, three-drug combination chemotherapy with Adriamycin, especially that including cis-platinum, was effective against transitional cell carcinoma of the urinary tract, but the results were not completely satisfactory.

Adult↗
PubMed1983

An amber replication mutant of F plasmid mapped in the minimal replication region.

We have constructed a mini-F derivative (pKP1013) consisting of a 5.4 kilobase pairs (kb) segment (44.0 to 49.4 kb) of mini-F and fragments carrying the chloramphenicol and spectinomycin resistance genes that originated from the R plasmid NR1. The plasmid pKP1013 replicates autonomously in a manner indistinguishable from that of the parental mini-F. An amber mutant defective in replication has been isolated from pKP1013 by localized mutagenesis using N-methyl-N'-nitro-N-nitrosoguanidine. The virtual absence of incorporation of [3H]-thymidine into the plasmid DNA as well as the kinetics of appearance of plasmid-free segregants suggest that plasmid DNA synthesis is primarily affected under nonpermissive conditions. The amber mutation has been mapped within the 530 base pairs (bp) region that extends from 45.25 (XmaI) to 45.78 Kb (PstI) by extensive analysis of in vitro recombinants constructed from rep+ and rep- plasmids.

Chromosome Mapping↗
PubMed1983

[Studies on the effects of beryllium oxide in purity and fired temperature to the occurrence of chronic pulmonary berylliosis].

Among various beryllium (Be) compounds, beryllium oxide (BeO) has so far been noted as a causative substance which introduce chronic pulmonary berylliosis. In our study, chronic pulmonary berylliosis was experimentally evoked by using two species of BeO with individual purity and fired temperature (A group: 95.0% in purity, and 1,300 degrees C or above in fired temperature, B group: 99.9% in purity, and 800-1,000 degrees C in fired temperature), and demarcation of prepared Be concentration involving in the genesis of the disorder was examined. BeO with various concentrations ranging from 200 micrograms to 0.1 micrograms were mixed with 0.1% agar solution and infused into right thorax cavity of male guinea pig, following which variation of the body weight was checked and pulmonary histo-pathological findings, peripheral hematological tests, and sero-biochemical tests were carried out on a periodical basis at 8 weeks, 16 weeks, and 32 weeks. The results obtained are outlined herein. 1) Body weights of test animals in both A group and B group were significantly decreased as compared with that of the control. 2) Pulmonary histo-pathological findings revealed the genesis of epitheloid-cell granuloma in the lungs where non-infusion sides were included, which resembled to non-caseating granuloma in the human pulmonary berylliosis. 3) The frequency in the genesis of epithelaid-cell granuloma was 1/36 (2.8%) in A group and 4/35 (11.4%) in B group, granuloma formation being marked in the latter. 4) Examination of the frequency in terms of BeO dosages revealed no results suggesting the dose-response relationship between pulmonary granuloma formation and the dosage. 5) There were no changes worthy of note in peripheral hematological tests. 6) Sero-biochemical tests revealed that alkaline phosphatase value together with total protein in each was significantly decreased in B group at 32 weeks following the infusion.

Berylliosis↗
PubMed1983

The complete nucleotide sequence of the influenza virus neuraminidase gene of A/NJ/8/76 strain and its evolution by segmental duplication and deletion.

The neuraminidase (NA) gene from A/New Jersey (NJ)/8/76 (H1N1, formerly Hsw1 N1) strain isolated in 1976 was cloned into pBR322 and its complete nucleotide sequence was determined. The NJ8 NA gene is 1458 nucleotides long and the sequence predicted the primary structure of the NA molecule comprising of 469 amino acids with a molecular weight of 51,628. Comparison with other NA sequences of the N1 subtype strains which were isolated in 1933-1934 identified the highly variable regions at the amino-terminal stalk region and the carboxy-terminal regions. Potential glycosylation sites encoded by a 15 base-pair unit sequence are arrayed tandemly at the stalk regions. The lengths of stalk regions are highly variable because of segmental deletions in old NA genes. Possible mechanisms for such deletions are discussed.

Animals↗
PubMed1983

[Experience with transabdominal and scrotal ultrasonographic examination in urology].

To evaluate its diagnostic availability in urology, transabdominal and scrotal ultrasonographic examination was made on 87 patients who had been admitted to our hospital for space occupying lesions. In retroperitoneal lesions, adrenal tumors and renal tumors greater than 2.5 cm could be detected by this examination. In pelvic lesions, bladder tumors greater than 0.5 cm were demonstrated and prostatic carcinoma with heterogeneous echotexture and irregular margin was discriminated from BPH. Not only could fluid-filled scrotal lesions larger than 1.0 cm be distinguished from solid mass lesions, but the intrascrotal anatomy could also be demonstrated in detail. Because of its safety, flexibility, and accuracy in detecting space occupying lesions, this examination could be a useful screening test in urology. Representative cases we experienced are presented.

Abdomen↗
PubMed1982

Translocation of colicin E1 through cytoplasmic membrane of Escherichia coli.

The product of the malE-lacZ gene fusion was reported to compete with some proteins including outer membrane lipoprotein in the protein translocation across the Escherichia coli membrane. The fusion product also inhibited colicin E1 export. Furthermore, globomycin, which accumulated prolipoprotein in the membrane, inhibited the translocation of colicin E1 in the wild-type cells, but not in lipoprotein-negative mutant cells. Since colicin E1 contains the internal signal-like sequence [Proc. Natl. Acad. Sci. USA (1982) 79, 2827-2831], these results suggest that colicin E1 is exported by the aid of this sequence at a common site for maltose-binding protein and lipoprotein translocation.

Biological Transport↗
PubMed1982

Investigation of genetic markers in a true hermaphrodite with chi 46,XX/46,XY.

We documented a new case of chi 46,XX/46,XY true hermaphroditism substantiated by the evaluation of chromosomal heteromorphism in banded preparations. The patient, a 12-year-old Japanese boy with ambiguous external genitalia, was seen because of abnormal breast development. Surgical exploration showed the right gonad to be an ovotestis and the left gonad to be an ovary. Cytogenetic studies revealed cell admixtures of 46,XX and 46,XY karyotypes in peripheral lymphocytes, skin fibroblasts, and gonadal fibroblasts. From the pedigree studies, the paternal double genetic contributions were evidenced by the differences of sex chromosomes and the blood group types for the ABO and MNSs systems in the two cell lines of the patient. The maternal double genetic contributions were confirmed by the inheritance of Q-fluorescent markers on chromosomes 13 and 22 and by alleles for the Kidd blood group system.

Blood Grouping and Crossmatching↗
PubMed1982

Scanning electron microscopic observation of glial cells following xenon arc photocoagulation.

The retinal tear produced in rabbits by aspirating the vitreous body was treated by means of photocoagulation, and its time-course changes were observed under a scanning electron microscope. Four weeks after photocoagulation, the surface of the retina surrounding the tear began to swell glial cells, that protruded with the lapse of time. About 6 weeks after photocoagulation, the glial cells perforated the internal limiting membrane of the retina and came out its surface, so that the glial cell processes and the cell perikaryon could be clearly observed morphologically. The findings showed that the glial cells are fibrous astrocytes. Seven weeks after photocoagulation, glial cells proliferated even in the retinal tear and, furthermore, glial cells in the space between the retinal tear and the retina surrounding it joined together by intertwining their processes to form a network, which seemed to play a certain role in repairing the tear.

Animals↗
PubMed1982

Cloning of human immunoglobulin epsilon chain genes: evidence for multiple C epsilon genes.

An active human epsilon chain gene was cloned from a phage library containing partial EcoRI digests of IgE-producing myeloma DNA, using the human JH (joining) gene fragment as a probe. The epsilon chain gene clone was identified by partial nucleotide sequence determination. The germ-line constant region gene of the epsilon chain (C epsilon gene) was cloned from a human fetal liver DNA library, using the cloned epsilon chain gene as a probe. Comparative studies on the human and mouse germ-line epsilon chain genes revealed that the switch (S) sequence is more conserved than the coding sequence. Restriction endonuclease BamHI digestion of human DNA produced three C epsilon fragments of 3.0, 6.5, and 9.2 kilobases, which were named C epsilon 1, C epsilon 2, and C epsilon 3 genes, respectively. We found the three C epsilon gene fragments in all of the human DNA preparations from eleven individuals. The C epsilon gene expressed in the myeloma was identified as the C epsilon 1 gene. Because the C epsilon 2 gene is deleted from the myeloma DNA, the order of the C epsilon genes is likely to be 5'-C epsilon 2-C epsilon 1-C epsilon 3-3', assuming that all the C epsilon genes are on chromosome 14. The germ-line C epsilon 3 gene was also cloned from the myeloma DNA. Characterization of the C epsilon 3 gene revealed that it does not have the S region, suggesting that it might be a pseudogene.

Base Sequence↗
PubMed1982

Purification and characterization of a protein from Escherichia coli which forms complexes with superhelical and single-stranded DNAs.

From the cells of an Escherichia coli K-12 strain, a 22,000-dalton protein which has an affinity for the superhelical DNA molecule was purified to apparent homogeneity by monitoring the DNA-binding activity using the filter binding assay. In the sedimentation analysis of the DNA-protein complex, the protein has an affinity for the superhelical or single-stranded DNA molecule but neither for the open-circular nor for the linear DNA molecule. The amino acid composition of the protein resembled those of the other prokaryotic histone-like proteins and also to eukaryotic histones H2A and H2B. The protein precipitated upon heating, which is in contrast to the heat-stable feature of the other histone-like proteins. Furthermore, DNA and RNA syntheses in vitro were not affected by the presence of the protein. In view of these characteristics, this protein may play a role in maintaining the bacterial nucleoid structure.

Bacterial Proteins↗