Nucleotide sequence of a cDNA for 1-aminocyclopropane-1-carboxylate synthase from melon fruits.
Explore the source record for details and available documents.
Base Sequence↗
Biomedical subjects
T Miki
Publications and source records attributed to T Miki.
Biallelic DNA rearrangements and deletions within the BCL-6 gene in B-cell non-Hodgkin's lymphoma.
Chromosomal translocations involving band 3q27 are recently described common specific cytogenetic abnormalities in B-cell neoplasms, and the BCL-6 gene, identified on 3q27, was shown to be disrupted and over-expressed in lymphoma cells having these chromosomal translocations. In the present study we found rearrangements within the BCL-6 gene in seven out of 35 cases with B-cell non-Hodgkin's lymphoma (NHL). Further analysis revealed that three of these patients with BCL-6 abnormality had multiple rearranged bands hybridized with probes from a single restriction fragment within the major translocation cluster (MTC), suggesting that independent DNA rearrangements would occur on both alleles. Additionally, Southern blot analysis indicated that three patients carry deletions encompassing the area containing the first exon of the BCL-6 gene. Our results suggest that biallelic DNA rearrangements and deletions would occasionally occur in NHL patients with BCL-6 abnormality.
Adult↗
Treatment with granulocyte colony-stimulating factor for pneumonia in a patient with a variant form of X-linked chronic granulomatous disease.
Explore the source record for details and available documents.
Adult↗
Kinetic characterization of the redox components in solubilized membranes from porcine neutrophils: reduction with dithionite and photoexcited NAD(P)H.
Cytochrome b558 in solubilized membranes prepared from porcine neutrophils was reduced by dithionite with a second-order rate constant of 2.5 x 10(6) M-1 s-1 at pH 7.4 and 20 degrees C accompanied by spectral changes with peaks at 428 nm and 560 nm and isosbestic points at 420 and 441 nm. When an anaerobic mixture of solubilized membranes and NAD(P)H was exposed to a white light, cytochrome b558 was reduced biphasically but with almost the same spectral profiles as in the dithionite reduction. Thus, participation of redox component(s) of unknown nature in the photochemical reduction was suggested. The NAD(P). radical generated by photoexcitation of NAD(P)H with a 355 nm laser pulse under anaerobic conditions also reduced cytochrome b558 with a high rate constant of 4.3 x 10(8) M-1 s-1 at pH 7.4 and 20 degrees C. The reduction of cytochrome b558 accompanied a simultaneous reduction of a component having an absorption band around 420 nm, suggesting participation of an iron-sulfur (Fe-S) cluster. The cytochrome b558 reduction was followed by its reoxidation by another component with an apparent second-order rate constant of 6.5 x 10(5) M-1 s-1. During the reoxidation, the Fe-S-like component remained in the reduced state, and thus its role other than as electron mediator in neutrophils NADPH oxidase is suggested. Not only the rate constant but also the extent of cytochrome b558 reoxidation decreased as the same reaction mixture was exposed to the laser pulse repeatedly. This result clearly indicates that an electron accumulates in this electron-accepting component designated tentatively as the omega component.
Animals↗
Partial purification and substrate specificity of flavin-containing monooxygenase from rat brain microsomes.
Flavin-containing monooxygenase (FMO) was partially purified from rat brain microsomes through two successive chromatographies on columns of DEAE Sepharose and 2',5'-ADP Sepharose. The specific activity, benzydamine N-oxidation of partially purified brain FMO, was 122-fold higher than that of microsomes. A single band of 60 kDa was recognized by Western blotting analysis with anti-rat liver FMO. The Km value of brain FMO for thiourea was 4-fold lower, but that for cysteamine was 10-fold higher than that of liver FMO. The enzymatic activity for n-octylamine was detected in neither brain nor liver FMO. Kinetic analysis for neurotoxins also revealed that Km values of brain FMO for 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), 1,2,3,4-tetrahydroisoquinoline (TIQ) and N-methyl TIQ (NMTIQ) were lower than those of liver FMO. These results indicate that rat brain FMO catalyzes several substrates of liver FMO involving neurotoxins, but it seems likely that the kinetic properties of brain FMO are somewhat different from those of liver FMO.
Animals↗
Insulin receptor gene polymorphism and hyperinsulinemia in hypertensive patients.
Although insulin resistance often occurs in association with hypertension, considerable variation is observed in the degree of insulin resistance among hypertensive patients. Since there is evidence of a genetic basis in the development of insulin resistance in hypertension, we analyzed the contribution of genetic factors to insulin resistance in hypertensive patients. Sixty-six Japanese hypertensive patients were studied. These patients were divided into two groups (hyperinsulinemia group and normoinsulinemia group) according to plasma insulin response during a 75-g oral glucose tolerance test (75g-OGTT). Insulin receptor gene (INSR) was studied for association with insulin resistance in hypertensive patients. A microsatellite polymorphism in intron-2 of the insulin receptor gene was analyzed by the polymerase chain reaction method. Five alleles were detected in the INSR microsatellite. The frequency of C/C genotype in the hyperinsulinemia group was significantly higher than that in the normoinsulinemia group (73% vs. 43%, p = 0.02). There was no difference in genotype frequency of INSR between hypertensive patients and control subjects. When the hypertensive patients were divided into two groups, the frequency of C/C genotype in the hyperinsulinemia group was significantly higher than that in the control group (73% vs. 45%, p = 0.014). There was no significant difference between the normoinsulinemia group and control group. These data suggest that the insulin receptor gene may contribute to insulin resistance in hypertensive patients with hyperinsulinemia.
Alleles↗
Gap junction protein locus on chromosome 18 cosegregates with body weight in the spontaneously hypertensive rat.
To detect genetic predisposing factors for hypertension, we screened the genome of the spontaneously hypertensive rat (SHR). We determined the genotypes of all F2 rats at polymorphic sites between the two strains, using the DNA of F2 rats derived from mating of SHR and Wistar-Kyoto rats (WKY). Cosegregation analysis was conducted to assess whether the genotypes of polymorphic markers associate with any phenotypic parameters such as directly measured blood pressure, heart rate or body weight. All measurements in F2 rats were performed at 15 weeks of age. Two polymorphic markers on chromosome 18 cosegregated with body weight. Gap junction protein (heart connexin 43) gene showed the most significant association with body weight as a recessive trait, but no association was noted with other parameters. We conclude that the gap junction protein locus is a new candidate for the determinant gene of body weight in SHR.
Analysis of Variance↗
Morphological demonstration of the immune privilege in the testis using adjuvants: tissue responses of male reproductive organs in mice injected with Bordetella pertussigens.
The testis, the epididymis and the prostate are immunologically suppressed organs in which allogeneic tissue grafts can survive for a long time. In the present study, morphological features of these three organs after systemic administration of immunopotentiators was investigated in mice to determine whether or not this treatment can affect their immunosuppressive circumstances. The animals were intravenously injected with adjuvants, Bordetella pertussigens, then killed 7-10 days later for histological examination. The results showed that the testicular interstitium was completely free from leukocyte infiltration but that the accessory glands (the prostate, the coagulating gland and the seminal vesicle), the vas deferens, the epididymis and the ductuli efferentes received extravasation of leukocytes into their interstitial tissues. This indicates that the testis is resistant to leukocyte infiltration compared with the epididymis and the prostate.
Adjuvants, Immunologic↗
Determination of a common clonal origin of gastric and pulmonary mucosa-associated lymphoid tissue lymphomas presenting five years apart.
Mucosa-associated lymphoid tissue (MALT) lymphoma is often mis-diagnosed as a benign tumor. Dissemination to other sites occurs in MALT lymphoma. We report a 60-year-old man with gastric and pulmonary tumors of MALT lymphoma which occurred 5 years apart. Initially, the gastric tumor had been diagnosed as reactive lymphoreticular hyperplasia. To determine whether the two tumors arose from the same malignant clone, we amplified and sequenced the complementarity-determining region 3 of the immunoglobulin heavy chain gene using the polymerase chain reaction (PCR). The sequences were identical except for 11-nucleotide difference, suggesting identical clonality.
Base Sequence↗
Leukemia developing after 131I treatment for thyroid cancer in a patient with Werner's syndrome: molecular and cytogenetic studies.
A 40-year-old female patient with Werner's syndrome (WS) suffering from thyroid cancer and myelodysplastic syndrome (MDS) is reported. She had been diagnosed as having WS complicated with thyroid cancer seven years previously. Total thyroidectomy and radioactive iodine (131I, 100 mCi/year) therapy for seven years had slowed the progression of thyroid cancer. She suffered a sudden onset of MDS at the age of 40 years. After six months she died from overt leukemia. We found an additional chromosome aberration of chromosome 10 in the progression of leukemia from MDS.
Adenocarcinoma, Papillary↗
Midbrain paralemniscal projections to the facial nucleus: an anatomical and immunohistochemical study.
Serial 30 microns-thick sections through the midbrain tegmentum were stained with cresyl violet. The PL was found to be situated along the medial edge of the lateral lemniscus. The PL consisted of small- (10-15 microns) and medium-sized neurons (25-35 microns), and was the most prominent at the caudal level of the superior colliculus. In order to confirm the existence of the inhibitory paralemniscal-facial pathway, a combined HRP and immunohistochemical technique was use in the rat. This experiment revealed that 10.9% of the total number of GABA immunoreactive PL neurons also labeled with HRP after HRP injection was made in the medial part of the facial nucleus (FN). Electron microscopic observations were carried out on the medial part of the facial nucleus (FN) after kainic acid injection was made into the contralateral PL in the cat. The majority of degenerating PL fibers were ranged from 0.5 to 3.1 microns in diameter and made synaptic contacts with somata, proximal dendrites and dendritic profiles. These fibers, containing either round or pleomorphic vesicles, formed asymmetrical or symmetrical synapses. It was of particular interest in the present study that 40.7% of the total number of degenerating fibers make synaptic contacts with large dendrites more than 3.0 microns in diameter.
Animals↗
Ultrastructure of spinal relay of hypoglossal afferents to the parabrachial nucleus.
Wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP) injection into the hypoglossal nerve mainly resulted in retrograde labeling in the superior ganglia of the glossopharyngeal and vagal nerves ipsilaterally. Anterogradely labeled fibers were found in lamina I of the ipsilateral upper cervical spinal cord with a few distribution to laminae IV-V and VII-VIII. WGA-HRP injection into the PBN revealed intensive labeling of lamina I neurons of the upper cervical spinal cord ipsilaterally. These light microscopic observations appear to indicate the hypoglossal sensory inputs to the PBN through the spinal cord. In order to investigate the synaptic nature of this spinal relay, electron microscopic observations were carried out on lamina I of the first and second cervical spinal cord after cutting the hypoglossal nerve and WGA-HRP injection into the PBN in the same animal. The spinoparabrachial projection neurons were demonstrated to show a low cytoplasmic/nuclear ratio and have an oval or deeply indented nucleus with a centrally located nucleolus. Furthermore, dark and light type degenerating fibers were observed to make synaptic contacts with HRP-labeled somata and dendritic profiles.
Afferent Pathways↗
Hypoglossal afferents to lamina I neurons of the cervical spinal cord projecting to the parabrachial nucleus in the cat.
Attempts were made to determine the hypoglossal sensory inputs to the parabrachial nucleus (PBN) through the spinal cord. Wheatgerm agglutinin conjugated to horseradish peroxidase (WGA:HRP) was injected into the cat hypoglossal nerve. HRP-labeled fibers, predominantly derived from the glossopharyngeal and vagal nerves, were observed to terminate in lamina I of the upper cervical spinal cord. A few fibers were also distributed to laminae IV-V and VII-VIII ipsilaterally. WGA:HRP injection into the lateral portion of the PBN also resulted in retrograde labeling in lamina I with ipsilateral predominance. Light-microscopic data raised the possibility of a relay of hypoglossal sensory information to the PBN in lamina I of the cervical spinal cord. In order to confirm the spinal relay, electron-microscopic observations were carried out on lamina I of C1 spinal cord after sectioning of the hypoglossal nerve and WGA:HRP injection into the lateral portion of the PBN on the same side in each animal. It was of particular interest that degenerated hypoglossal afferent fibers made synaptic contacts with lamina I neurons, which were retrogradely labeled with HRP.
Afferent Pathways↗
[Genetic analysis of Werner syndrome in a family].
Werner syndrome (WRN) is a rare autosomal recessive disorder, one of the progeroid syndromes, and is characterized by features of premature aging. The incidence of WRN in the Japanese population, 1 in 200,000, is higher than than that in the Caucasian population. The genetic defect of WRN is unknown. But genetic linkage to several markers on the short arm of chromosome 8 has been reported recently. Here, we studied one family with WRN in which an affected individual had a papillary thyroid carcinoma and myelodysplastic syndrome. Using 4 microsatellites closely located to the WRN locus: D8S360, D8S1055, D8S339 and ANK1, we analyzed the genotypes of this patient, her three siblings and her parents, who were first cousins. The mutative haplotype, identified through the generations in pedigree, helps detect a carrier or a presymptomatic patient. The eldest sister inherited two normal haplotypes, but the second sister inherited one mutative haplotype. There was no difference in clinical signs and symptoms between these sisters. when the WRN gene is isolated, it will help us understand the mechanism of aging.
Adult↗
Growth of parathyroid gland in uremic patients on maintenance hemodialysis.
High-resolution, real-time ultrasonography was performed in 245 uremic patients on maintenance hemodialysis and the growth of the enlarged parathyroid glands was compared with the clinical and biochemical signs during follow-up periods of 12 months. The total volume of the parathyroid glands was significantly correlated with the serum C-terminal parathyroid hormone (C-PTH; r = 0.379, p = 0.0001) and calcium levels (r = 0.252, p = 0.0224). After 12 months, the total volume of the enlarged parathyroid glands and serum C-PTH or calcium levels were correlated more closely than in the initial study (r = 0.615, p = 0.0001, and r = 0.489, p = 0.0002, respectively). Both the serum C-PTH levels and the total volume of the enlarged parathyroid glands increased significantly (p = 0.0001), while the ratio between the serum C-PTH levels and the volume of the parathyroid glands decreased significantly (p = 0.0001). There was no difference in the clinical and biochemical signs except for the serum aluminum levels between the patients with and without an increased gland volume. These results suggest that the growth of parathyroid glands may progress more rapidly than the increase in the serum PTH levels, independent of the serum calcium levels, in uremic patients on maintenance hemodialysis.
Adolescent↗
Influence of serum phosphate on the efficacy of oral 1,25-dihydroxyvitamin D3 pulse therapy.
In patients with a moderate degree of renal insufficiency, restriction of dietary phosphate suppresses PTH secretion by increasing serum calcitriol. However, this may not operate in advanced renal failure. The present study was designed to evaluate the influence of serum phosphate levels on PTH secretion in oral 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] pulse therapy. 22 patients with secondary hyperparathyroidism [carboxy-terminal PTH (c-PTH) concentration: 19.5+/-13.9 ng/ml, mean +/-SD] received oral doses of 1,25(OH)2D3 (3.0-4.0 micrograms) twice a week, each at the end of hemodialysis, for 12 weeks. Doses of phosphate binders remained unchanged throughout this period. Patients were divided into two groups: group A (11 subjects) with mean serum phosphate levels of less than 6.0 mg/dl and group B (11 subjects) with levels of 6.0 mg/dl and above. There was no significant difference in the average corrected serum calcium levels. The reduction in serum intact PTH levels was greater in group A than in group B. A negative correlation (r = -0.48; p < 0.05) was observed between mean serum phosphate levels and the percent decrease in serum c-PTH levels. The findings of this study indicate an important role for dietary phosphate reduction in oral 1,25(OH)2D3 pulse therapy and suggest that serum phosphate reduction may play a part in suppressing PTH secretion through a mechanism independent of 1,25(OH)2D3 and plasma calcium levels.
Administration, Oral↗
[Clinical studies of 15 cases of renal angiomyolipoma].
Fifteen cases of renal angiomyolipoma seen between May, 1988 and October, 1994 in our hospital are presented. Surgical treatment was performed in 6 cases, 1 of which was falsely diagnosed as liposarcoma by frozen specimen during the operation. Nine cases were only followed-up by ultrasonography and/or computed tomography. In 2 cases, in which enucleation was performed after a follow-up period of more than one year, tumors were inclined to grow rapidly. In 5 cases, which were not operated and followed-up for more than one year, tumors were inclined to grow very slowly.
Adult↗
[Giant schwannoma in the pelvic cavity presenting as renal failure: a case report].
A case of giant schwannoma in the pelvic cavity detected with renal failure is reported. A 50-year-old man was referred to another clinic with chief complaints of general fatigue and edema of the face and dorsa of the feet. On March the 4th 1994, he was hospitalized in the clinic because of renal failure. Bilateral hydronephrosis and a giant pelvic tumor were found by computed tomography (CT) and ultrasonography. Because bilateral percutaneous nephrostomies failed to recover his renal function, he was referred to our clinic for the purpose of hemodialysis and the further examination of the tumor on March 16, 1994. The pelvic angiography showed that the tumor was fed by the vessel from the left internal iliac artery. After the chemo-embolization from the feeding artery, tumor resection was performed on May 9, 1994. The tumor was 16x13x10 cm in size, and 1,110 g in weight. The histological findings of the tumor revealed the mixed type schwannoma of Antoni A and B. Six months after the operation, he has had no tumor recurrence. This is a rare case of pelvic schwannoma which was detected with renal failure. We reviewed and discussed 56 cases of schwannoma in the pelvic cavity, including our case, in the Japanese literature.
Humans↗