Search PubMed⌕ Search

Biomedical subjects

T Mikami

Publications and source records attributed to T Mikami.

815 records · Page 46Linked to original sources
PubMedDate not supplied

Blocking of immunofluorescence in the titration of membrane and intracellular reactive antibodies associated with herpesvirus of turkeys.

Blocking of the direct immunofluorescence (IF) reaction allowed titration of anti-membrane antigen (MA) or anti-intracellular antigen (IA) antibodies induced by herpesvirus of turkeys (HVT). The titers (blocking index) obtained by the blocking IF test were well correlated with those determined by the IF test. The results suggest that the blocking IF test was a simpler and better method for titration of a large number of serum samples from chickens infected with HVT.

Animals↗
PubMedDate not supplied

Detection and classification of infectious bronchitis viruses isolated in Korea by dot-immunoblotting assay using monoclonal antibodies.

Dot-immunoblotting assay (DIA) using five monoclonal antibodies (MAbs) to infectious bronchitis virus (IBV) was used to detect and classify the viruses propagated in embryonated chicken eggs. Using a group-specific MAb 3F5, 10 reference strains and 12 Korean isolates of IBV were successfully detected by DIA, and the lowest virus titer of IBV detected by DIA was approximately less than 10(3.8) mean embryo infective dose/ml. For evaluating the diagnostic efficiency, DIA was compared with the conventional infectious bronchitis (IB) diagnostic method. IBV antigens in allantoic fluid from embryonated eggs inoculated with IB-suspected field samples were specifically detected by DIA within only one or two egg passages, whereas the conventional embryonated egg inoculation method required four to seven egg passages for confirming IBV infection. These results indicated that DIA could significantly reduce time and cost for IB diagnosis. For examining the possibility of classifying IBV by DIA, four strain-specific MAbs, 3A4, 2A3, 6F7, and 2C6, were used. According to the MAb reacting patterns to the IBV antigens, the 10 IBV reference strains were classified into six groups; seven strains belonged to three different groups, and the other three strains each belonged to an individual group. In the case of 12 Korean isolates of IBV, they were classified in six groups. Among the six groups, the MAb reacting patterns of three groups matched those of the IBV reference strains, but the others did not. These data suggest that at least three variant serotypes of IBV exist in Korea.

Allantois↗