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T Matsuto

Publications and source records attributed to T Matsuto.

At least 37 records · Page 2Linked to original sources
PubMed1996

[Usefulness of PCR-SSCP using non-RI coloration method as a routine genetic analysis].

The polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis is considered as one of useful routine genetic analyses. However, that PCR-SSCP has been analyzed using radioisotope (RI) such as [32P] labeled compounds. So that, it was difficult to use this method routinely at clinical laboratories. In this paper, we compared with an RI mediated and a non-RI coloration mediated PCR-SSCP analysis regarding to those of sensitivity, time and money cost, respectively. The coloration method had enough sensitivity to detect one of cholesteryl ester transfer protein gene mutations. The time and money cost of non-RI coloration mediated method were more advantageous than the RI mediated one. Therefore, it is shown that the non-RI coloration mediated PCR-SSCP analysis is very useful for the routine genetic analysis at clinical laboratories.

Carrier Proteins↗
PubMed1996

[Abnormality of HDL subfractions in cholesteryl ester transfer protein deficiency].

High-density lipoprotein (HDL) in cholesteryl ester transfer protein (CETP) deficiency is deficient in reverse cholesterol transport although HDL-cholesterol levels markedly increase in such patients. This may be responsible for the abnormal metabolism of pre beta 1-HDL, the initial acceptor of cell-derived cholesterol. To answer this question, we determined HDL subfraction levels in 8 CETP deficiency and 21 normolipidemic subjects. We also measured the rate of decrease in pre beta 1-HDL level by LCAT activity during the incubation at 37 degrees C for 90 min. We found that HDL2b was 2.4 times higher in CETP deficiency than in control [52.2 +/- 14.3, vs. 21.5 +/- 9.8% of plasma apoA-I(%AI), p < 0.005] while HDL2a and HDL3 were significantly lower in the former than in the latter (HDL2a; 25.5 +/- 8.0 vs. 40.4 +/- 6.5% AI, p < 0.005) (HDL3; 10.0 +/- 6.8 vs. 28.3 +/- 5.9% AI, p < 0.005). There were no significant differences in pre beta 1-HDL level between 2 groups (4.9 +/- 3.0 vs. 4.5 +/- 2.3% AI, NS). However, the rate of decrease in pre beta 1-HDL was significantly less in CETP deficiency than in control (-33.2 +/- 22.9 vs. -73.7 +/- 12.2%, p < 0.005). In conclusion, the metabolism of pre beta 1-HDL is delayed in CETP deficiency. It is suggested that the esterification of cell-derived cholesterol removed by pre beta 1-HDL may be deficient in CETP deficiency.

Apolipoproteins↗
PubMed1996

Pre beta 1-high-density lipoprotein increases in coronary artery disease.

Pre beta 1-HDL promotes cholesterol efflux from cell membranes. Its plasma concentration is regulated by lecithin: cholesterol acyltransferase (LCAT). To clarify whether the concentration of pre beta 1-HDL changes in coronary artery disease (CAD), we determined the distribution of apolipoprotein A-I (apoA-I) among HDL subfractions in 20 CAD patients and 20 healthy controls, using nondenaturing two-dimensional gel electrophoresis. We found that CAD patients had significantly higher concentrations of pre beta 1-HDL than the controls [7.6% +/- 3.4% vs 4.6% +/- 2.3% of apoA-I (P < 0.01)]. Even after correcting for apoA-I concentrations, this increase remained significant [87 +/- 37 vs 63 +/- 28 mg/L apoA-I (P < 0.05)]. The mean LCAT concentration was significantly lower (P < 0.05) in CAD patients than in controls. These findings, that pre beta 1-HDL concentrations increase in CAD, strongly suggest that the process of reverse cholesterol transport could be altered in CAD.

Aged↗
PubMed1995

Effects of modified low density lipoprotein and hypoxia on the expression of endothelial leukocyte adhesion molecule-1.

OBJECTIVES: Expression of endothelial leukocyte adhesion molecule (ELAM-1 or CD62E) plays a role as an early event of atherogenesis. It is well known that interleukin-1 (IL-1) expresses ELAM-1 on vascular endothelial cells. We have examined pathological factors that induce ELAM-1 expression on cultured endothelial cells. METHODS: Examined factors were native low density lipoprotein (LDL), oxidized LDL, glycated LDL, hypoxia, and IL-1. Peroxidation of LDL was performed by ultraviolet radiation. Hypoxia was reproduced by adding a hypoxic cell-culture medium that was deoxygenated by use of a vacuum pump and nitrogen gas. Endothelial cells were harvested from a porcine aorta and were allowed to proliferate to be subconfluent in slide chambers. Expression of ELAM-1 was evaluated by counting the number of cells that were characterized by positive staining with the immunohistochemical technique. RESULTS: Without any stimulants, about 6.9% of the endothelial cells expressed ELAM-1. Weakly oxidized LDL (12 pmol/micrograms protein) significantly expressed ELAM-1 (14.8%) after an incubation period of 1 hour. Glycated LDL induced significant expressions (12.6%) in a fructosamine concentration of 65 pmol/micrograms protein. A one-hour incubation with a hypoxic culture medium expressed ELAM-1 in 16.3% of the cells. Native LDL did not cause any significant increases in the percentage. IL-1 expressed ELAM-1 in 30% of the cells even with as low a concentration as 3.1 U/ml. CONCLUSIONS: The present study shows that not only IL-1 but also weakly oxidized LDL, glycated LDL, and hypoxia may be possible factors that cause the expression of ELAM-1.

Animals↗
PubMed1995

A new enzymatic method for the determination of inulin.

A new enzymatic method for the determination of inulin in plasma and urine, using inulase (EC 3.2.1.7), fructokinase (EC 2.7.1.4), phosphoglucoisomerase (EC 5.3.1.9) and glucose-6-phosphate dehydrogenase (EC 1.1.1.49) is described. The assay is based on the hydrolysis of inulin or Inutest (INutest which is the injectable form of inulin), by inulase and the determination of fructose released. The assay was linear up to 2 g/L of Inutest. The within-batch and between batch coefficients of variation were 2.3% and 2.2%, respectively. Recovery of added Inutest from plasma and urine was 98-102%. There was no interference from glucose (27.7 mmol/L), fructose (1.7 mmol/L) or mannose (1.7 mmol/L). When inulin clearance (using this method) and thiosulphate clearance were compared in 37 patients the inulin clearance was 9.3 mL/min (12%) lower than the thiosulphate clearance. We conclude that this enzymatic method is a simple and specific method.

Anthracenes↗
PubMed1994

[Calculation of normal range using a nonlinear least squares method].

We developed a method to calculate the normal range of laboratory tests using nonlinear least squares method. Our method decomposes a histogram of laboratory test into constituent distributions assuming these constituents to be gaussian distributions. The proposed method has been applied to the histograms of serum total protein and serum lactate dehydrogenase, and good fittings were obtained assuming the histograms consist of 2 and 3 constituents respectively. Accuracy and precision were calculated for the stimulated data of known distribution pattern with contamination of abnormal values to examine the robustness of our method. With the contamination rate of 10%, accuracy less than 1% and precision less than 3% have been shown for the sample size more than 2,000. And the estimation was not markedly affected by the contamination rate up to 50%. Based on the parameters estimated by our method, we proposed a new measure for laboratory tests named abnormality index, which gives a probability to be abnormal.

Clinical Laboratory Techniques↗
PubMed1993

[Detection of carbohydrate antigens of malignant cells in sputum with panel of monoclonal antibodies].

Serum tumor markers are useful for post-operative follow up, however, they are not necessarily useful for early stage diagnosis. Because the lesion is so small that it is unable to detect a tiny amount of their molecules in serum. If we could detect those antigens directly in cells from cytological specimens, it would provide a new diagnostic method for early stage cancers. The expression of carbohydrate antigens were examined with panel of specific anti-carbohydrate monoclonal antibodies (MAbs) on cytological specimens of sputum. In total, 146 sputa were collected in Sacomano's solution; 69 malignant cases (35 squamous cell carcinomas, 13 adenocarcinomas and 21 other primary lung cancers), 19 benign cases (pneumonia and bronchitis) and 58 borderline-malignancy cases which were defined by the standard of Japan Society of Lung Cancer. After removing mucus, the cells were stained with Vector's ABC method. Evaluation was performed by counting positively-stained cells among benign or atypical cells. As we examined previously in lung cancer tissue sections, there was statistical significance of frequency of positive stain between malignant and benign cases especially in MAbs AH6, THK2, SH1 and SNH3. Borderline malignancy gave intermediate value which means certain number of cells with cancerous biochemical character are mixed in the borderline specimens. In most cases, cell membrane was positively stained and sometimes, cytoplasm. Although the high sensitivity was observed in AH6 and SNH3, their specificity was lower than that of SH1, and visa versa. Those results indicate that the combination of anti-carbohydrate MAbs is useful for cytological diagnosis of lung cancer.

Antibodies, Monoclonal↗
PubMed1993

[Evaluation of serum protein fractions using nonlinear least squares method].

We developed a method to evaluate the contents of serum protein fractions using nonlinear least squares approach. Parameters that determined the distributions of fractions of a densitogram were estimated assuming that each fraction obeys Gaussian distribution. One hundred test results of patients and one hundred and fourteen simulated patterns were analyzed with the proposed method and the traditional method. For patient data, the traditional method tended to give higher values in beta-globulin fraction and lower values in alpha 2-globulin and gamma-globulin fractions compared with our method. For the simulated data, the traditional method also tended to give higher values in beta-globulin fraction and lower values in gamma-globulin fraction, whereas our method gave the correct values.

Blood Proteins↗
PubMed1993

[The improvement of colorimetric determination of lipid peroxide in human serum].

The present work details an improved application of kit to the measurement of lipid peroxides in human serum. Kits based on the reaction of lipid hydroperoxides with a leucomethylene blue derivative in the presence of hemoglobin. Overall sensitivity was increased by taking samples 5 times larger than those indicated in product literature for the system. It was necessary, however, to compensate actual data for protein error. To do this, the following empirically obtained formula was employed: A = a/(-3.45x + 98) x 100, where A (nmol/ml) indicates compensated data for lipid peroxides, a (nmol/ml) indicates the data for the actual measurement of lipid peroxides, and x (g/dl) indicates the total protein. We obtained what we considered to be satisfactory results by the above formula, as it allowed us to observe that lipid peroxides in fresh human serum undergo a change, momentarily.

Colorimetry↗
PubMed1992

[Use of heart rate variability and pulse wave velocity for diagnosing cardiovascular autonomic neuropathy].

Heart rate (HR) and arterial pulse wave velocity (PWV) were used for the evaluation of cardiovascular autonomic neuropathy. Data were analyzed from 30 patients with diabetes mellitus (aged from 13 to 75 years). Twenty healthy male subjects (aged from 22 to 44 years) were analyzed for computing normal values as well. After 15 minutes rest, electrocardiogram (ECG) of lead II and plethysmogram of finger tip were simultaneously recorded for each subject. The recording was first done in supine position for 120 seconds and subsequently in upright position for 40 seconds. HR was computed on the basis of consecutive pairs of R wave of the ECG. PWV was estimated by transit time from R wave peak to initial rise of pulse wave. Mean and coefficient of variation (CV) were obtained from the HR in the supine and upright positions respectively, and also from the PWVs. The influence of changing the position was evaluated by percentage of the difference (increasing rate). All the parameters were statistically tested for the difference between the patients with neuropathy and those without neuropathy. As the result, two parameters, that is, the CV of HR in the supine position and the increasing rate of PWV were found to be useful for diagnosing the cardiovascular autonomic neuropathy (p less than 0.05).

Adolescent↗
PubMed1990

[Personal laboratory data management system using optical card].

This paper describes the development and state of the medical optical card system. Recently, computerized medical information systems have been developed in many hospitals and they allow quick access and automatic processing of the patient's data in medical practice and research. However, the use of the information stored by such a system has been limited to each hospital while it is not rare for a patient to change hospitals. It is desirable to develop a personal medical data management system which allows the patient to carry his own medical records for a long period of time, and hospitals to share the information about the patient. The optical card is a transportable information medium with a large capacity. Since data are recorded optically with a laser beam, the card is tolerant to environmental factors such as static electricity, magnetism and impact which injure other transportable media such as IC card, magnetic stripe card and floppy disk. Therefore, the optical card suits our purpose. We developed a prototype of the medical optical card system. In our system, the card can contain character data, numerical data, two-valued image data and electrocardiographical data. In order to share laboratory data among different database systems, we defined a data descriptive language which enables complete and compact data description without any external code tables. The recorded patient data are presented in the original multi-window system, which allows the doctor to see any combination of any part of the patient's information simultaneously.

Clinical Laboratory Information Systems↗
PubMed1984

The excitatory action of the newly-discovered mammalian tachykinins, neurokinin alpha and neurokinin beta, on neurons of the isolated spinal cord of the newborn rat.

The actions of two new mammalian tachykinins, neurokinin alpha and neurokinin beta, were examined using the isolated spinal cords of newborn rats. Depolarizing responses of spinal motoneurons were recorded extracellularly from the lumbar ventral root during application of neurokinin alpha or neurokinin beta at concentrations ranging from 3 X 10(-8) M to 10(-6) M. The potencies of various tachykinins in depolarizing the motoneurons showed the following order: physalaemin greater than neurokinin beta divided by kassinin divided by substance P greater than neurokinin alpha. When the synaptic transmission in the spinal cord was blocked by tetrodotoxin, the depolarizing action of neurokinin alpha and neurokinin beta was markedly reduced but not completely abolished. The depolarizing action of neurokinin alpha and neurokinin beta was depressed by a substance P antagonist, [D-Arg1, D-Pro2, D-Trp7,9, Leu11]SP. The possibility that neurokinin alpha and neurokinin beta act as neurotransmitters in the mammalian spinal cord is discussed.

Animals↗