Search PubMed⌕ Search

Biomedical subjects

T Matsuno

Publications and source records attributed to T Matsuno.

At least 235 records · Page 13Linked to original sources
PubMed1985

[A histological study on osteosarcoma. Part II: The mode of local extension of osteosarcoma].

The survival rate of patient with osteosarcoma has been increasing, owing to the development of adjuvant chemotherapy. Recently, the limb saving procedure in osteosarcoma has been attempted in many countries. Although we need knowledge about the mode of local extension of osteosarcoma in the planning of this surgery, there are few reports concerning the matter. The purpose of this paper is to make clear, morphologically, how tumor tissue extends across the epiphyseal plate, periosteum and bone marrow. The materials were 26 amputated extremities of osteosarcoma which had not received any chemotherapy or radiotherapy. Paraffin embedded macrosections were prepared and stained with HE and Masson stain. When epiphyseal plate was open or closing, 13 out of 14 cases (93%) showed transphyseal extension of tumor. Tumor extension along the vessels at the center of the plate and the epiphyseal artery was most common. Transmedullary extension showed three different types, infiltrative type (18 cases), lobular type (5 cases), and "skip" type (one case). None had capsule or pseudocapsule surrounding the tumor. As to the relationship between periosteal reaction and tumor involvement of cortex or medulla, periosteal reaction was located more proximally than cortical or medullary involvement in some cases. In other cases, however, cortical or medullary involvement was located more proximally than periosteal reaction. The gaps between the tips of the periosteal reaction and cortical or medullary involvement ranged from 0.5 to 1.5 cm. In the transperiosteal extension, it was confirmed that tumor cells had invaded the periosteum along the vessels penetrating the periosteum.

Adolescent↗
PubMed1984

Eimeria tenella, E. necatrix, E. acervulina, and E. maxima: anticoccidial activity of 1,6-dihydro-6-oxo-2-pyrazinecarboxylic acid 4-oxide.

The anticoccidial activity of an orotic acid analog, 1,6-dihydro-6-oxo-2-pyrazinecarboxylic acid 4-oxide (carboxyemimycin), was tested in battery experiments, utilizing 9-day-old Single-Comb White Leghorn cockerels. Carboxyemimycin, at 125 ppm and more in feed, exhibited marked anticoccidial activities against Eimeria tenella, E. necatrix, E. acervulina, and E. maxima. High doses of carboxyemimycin--up to 1000 ppm--did not cause any reduction in weight gains. The battery and in vitro studies with delayed and restricted medications revealed that carboxyemimycin affected the development of E. tenella in first and second generation schizogony and in gametogony.

Animal Feed↗
PubMed1984

Muscular development in the urinary tract.

The structures of the ureter, ureterovesical junction, bladder and sphincteric urethra were investigated histologically in 17 human fetuses (from 3rd to 21st weeks) and 2 neonates, with particular emphasis on their muscular development. The differentiation of ureteral muscle begins from the upper part of the ureter at the 12th week and muscle bundles run spirally. The purely longitudinal intramural ureteral muscle differentiates much later than the upper ureteral muscle and begins to form muscle bundles at the 17th week. The deep periureteral sheath and the trigonal muscle differentiate at an almost similar pace to the intramural ureteral muscle. The detrusor muscle begins to differentiate from the apical dome of the bladder at the 7th week. Distinct superficial periureteral sheath is also seen around the 12th week. The urethral striated muscle is recognized at the 8th week. It starts to develop in the anterior wall of the urethra as a dense cellular accumulation which eventually extends caudolaterally to form a U-shaped figure. The urethral smooth muscle starts its differentiation between the 9th and 11th weeks inside the urethral striated muscle.

Cell Differentiation↗
PubMed1984

Pituitary and gonadal function in prepubertal boys with hypospadias.

Luteinizing hormone releasing hormone and human chorionic gonadotropin tests were performed to examine the pituitary gonadal axis in 31 prepubertal boys with hypospadias. Luteinizing hormone and follicle stimulating hormone responses to luteinizing hormone releasing hormone in these boys with hypospadias were significantly higher than those in prepubertal control subjects (luteinizing hormone, p less than 0.01). follicle stimulating hormone, p less than 0.05). Prepubertal boys with hypospadias had remarkably reduced testosterone responses to human chorionic gonadotropin stimulation compared to controls (p less than 0.01). Hormonal milieus were further analyzed in the subtypes of hypospadias depending on their severity (distal vs. proximal). Nine of the 31 boys with hypospadias were classified as the proximal type. Basal luteinizing hormone levels in the proximal type were significantly higher than in the distal type (p less than 0.05). Luteinizing hormone and follicle stimulating hormone responses to luteinizing hormone releasing hormone and responses of testosterone to human chorionic gonadotropin were not significantly different in the 2 types. Seven of the 31 boys with hypospadias had a history of maternal progestin ingestion. Basal luteinizing hormone levels and responses of luteinizing hormone and follicle stimulating hormone to luteinizing hormone releasing hormone were also significantly higher in these subjects than in controls (p less than 0.005), though basal levels and responses of testosterone to human chorionic gonadotropin were not different from those of controls. Tests conducted to determine luteinizing hormone and follicle stimulating hormone and testosterone levels using luteinizing hormone releasing hormone and human chorionic gonadotropin stimulation revealed no statistical differences between the boys with hypospadias who had a history of maternal progestin ingestion and those without such a history. The majority of prepubertal boys with hypospadias had varying degrees of deficient testicular activity. The testicular function of these patients should be evaluated longitudinally at puberty and thereafter in order to insure the completion of secondary sexual development.

Child↗
PubMed1984

So-called nasal glioma.

Nasal glioma is heterotopic brain tissue which usually presents as a tumor around the nose of children and infants. We have encountered two cases of nasal glioma during the past nine years at Hokkaido University Hospital. One showed a tumor in the subcutis of the nasal bridge and the other a polypoid mass arising in the soft palate. Both consisted of proliferation of fibrillary spindle cells consistent with fibrillary astrocytes divided by fibrovascular septa. This was reminiscent of "gliosis" of the central nervous system. Occasional protoplasmic astrocytes were present in both and, in addition, one case showed neuronal cells and choroid plexus. The glial nature of the lesion was confirmed by the presence of glial fibrillary acidic protein demonstrated by the immunoperoxidase method. Nasal glioma is not neoplastic contrary to its name and is considered heterotopic brain tissue which was displaced during fetal development by similar pathogenetic mechanisms of sincipital and basal encephalocele, although its communication to the brain was lost.

Astrocytes↗
PubMed1984

Rubella virus-induced interference in chick embryo cells.

Primary chick embryo cells inoculated with rubella virus were found to develop interference with the multiplication of Sindbis virus. Substantial quantities of interferon (IFN) were detectable in the cell cultures indicating that the resistance induced was due to IFN production.

Animals↗
PubMed1984

[A histological study on osteosarcoma. Part I: Relationship between modes of osteoid formation and differentiation of tumor cells].

This study was undertaken to clarify whether the osteoid forming activity seen in osteosarcoma can be used as an index to show the degree of differentiation of the tumor. Twenty six human osteosarcomas which had been amputated in our clinics were used in this study. The whole slice of the primary tumor was stained by HE and Masson stain. The following observations were microscopically made in the peripheral, transitional and central part of the tumor; 1) the content and maturity of osteoid, 2) the degree of polymorphism and mitotic activity of the tumor cells. In 13 out of 26 cases, the amount and maturity of osteoid gradually increased toward the center of the tumor. On the other hand, the degree of polymorphism and mitotic activity of tumor cells in these cases gradually decreased toward the center. Mitotic activity in the center of the tumor decreased to about half of the peripheral part. The clinical courses of these 13 cases were milder than those in which the gradual increase of the amount and maturity of osteoid toward the center of the tumor were not observed. These results showed that zone formation of osteoid maturity in osteosarcoma can be used as an index to show the degree of differentiation of the tumor.

Adolescent↗
PubMed1983

Activation of macrophage function by intraperitoneal administration of the streptococcal antitumor agent OK-432.

Motility, adhesiveness, IL1 production, and inhibition of tumor cell growth in vitro were examined in murine peritoneal macrophages obtained after intraperitoneal injection of a streptococcal preparation OK-432, heat-inactivated OK-432 (HI-OK-432), and thioglycollate medium (TG). By varying the interval between intraperitoneal injection of OK-432 and the harvest of peritoneal macrophages, it was found that OK-432 induced a time-dependent multi-step alteration of these properties: step I increased motility on day 1: step II increased adhesiveness on day 2; and step II increased inhibition of tumor cell growth and IL1 production. During step III, the peritoneal macrophage population, including Ia-bearing cells, increased dramatically in the peritoneal cavities of OK-432-treated mice. In contrast, injection of either HI-OK-432 or TG, which lack antitumor activity in vivo, initiated steps I and II, but not step III. The Ia-bearing macrophages induced by OK-432 showed high ability of IL1 production, but low growth inhibitory activity against tumor cells. Based on these results, OK-432 seems to be performing a dual function: eliciting a new population of macrophages to the site of injection (heat stable function), and inducing two different populations of antitumor macrophages and Ia-bearing macrophages (heat unstable function).

Animals↗
PubMed1983

Purification and comparison of glutamine synthetase from chicken liver, brain and neural retina.

Glutamine synthetase (L-glutamate: ammonia ligase, EC 6.3.1.2) was isolated from chicken liver, brain and neural retina. The specific activities of the purified enzyme preparations from the three different sources were similar. They were composed of subunits of the same molecular weight (43 K) and were immunologically indistinguishable. Slight differences were detectable among them in relation to the amino acid compositions and regulation of their activities by the several effectors tested.

Amino Acids↗
PubMed1983

Complete repair of severe penoscrotal hypospadias in 1 stage: experience with urethral mobilization, wing flap-flipping urethroplasty and "glanulomeatoplasty".

We describe our experience with complete repair of severe penoscrotal hypospadias in 1 stage. The operative technique includes several innovative points, such as release of chordee by urethral mobilization (without detaching it from the glans) after penile degloving of dartos chordee, wing flap-flipping urethroplasty to the tip of the glans and incrimination of "glanulomeatoplasty" for better cosmetic and functional results. Our technique is compared to other 1-stage methods.

Catheters, Indwelling↗