A new unstable hemoglobin with high oxygen affinity, Hb Toyoake: beta 142 (H20) Ala replaced by Pro, was found in Japanese male with a normal blood hemoglobin level, shortened red cell survival, and increased plasma erythropoietin. Hemoglobin studies showed heat and isopropanol instability, and an increased tendency to heme loss and to subunit dissociation. Electrophoresis of whole hemolysate showed inconstant abnormal bands with reduced mobilities due to progressive heme loss during the in vitro procedure. Isolated Hb Toyoake with normal heme content migrated slightly faster than HbA. Oxygen affinity of red cells was elevated with P50 of 17.0 mm Hg at pH 7.4 and 37 degrees C (normal 25.0 mm Hg). Studies on hemolysate implied that Hb Toyoake had an almost normal Bohr effect, a diminished cooperativity, and a reduced response to inositol hexaphosphate. These multiple abnormalities are associated with a substitution of Pro for beta 142 Ala, resulting in disruption of the H-helix and the adjacent C-terminal portion of beta chain, which contain residues crucial for normal oxygen binding.

Adult↗

PubMed1980

Multiple factors are required for the accurate transcription of purified genes by RNA polymerase III.

Cell-free extracts (S-100) prepared from cultured mammalian KB cells have previously been shown to direct accurate and selective transcription of class III genes by RNA polymerase III. We have fractionated the KB S-100 and have found that multiple components are essential for the accurate transcription of these genes. After the S-100 has been separated into four different protein fractions by chromatography on phosphocellulose, two fractions are required, in addition to RNA polymerase III, for active and selective transcription of the virus-associated RNAI gene of adenovirus 2 and a tRNA gene; a third fraction is required, along with these components, for the reconstitution of 5 S RNA gene transcription. At least two of these components are distinct from the four factors required for accurate initiation of transcription by RNA polymerase II (Matsui, T., Segall, J., Weil, P. A., and Roeder, R. G. (1980) J. Biol. Chem. 255, 11992-11996).

Carcinoma↗

PubMed1980

Multiple factors required for accurate initiation of transcription by purified RNA polymerase II.

A soluble extract prepared from human cells (KB S-100) has been recently shown to direct accurate transcription initiation by purified RNA polymerase II at the major late promoter of adenovirus 2. We have fractionated this extract by chromatography on phosphocellulose, DEAE-cellulose, and DNA-cellulose, and have identified four components that are required for the active and selective initiation of transcription by RNA polymerase II at this promoter. One of these components seems to act by suppressing random, but not selective, transcription by RNA polymerase II. All but one of these components have been shown to be chromatographically distinct from the factors involved in directing selective transcription by RNA polymerase III.

Carcinoma↗

PubMed1980

Experimental chronic non-suppurative destructive cholangitis in rabbits following immunization with bile duct antigen.

Experimental chronic non-suppurative destructive cholangitis induced in rabbits following immunization with bile duct antigen. Histological changes similar to chronic non-suppurative destructive cholangitis were induced in rabbits by sensitization with antigen prepared from bovine gallbladder mucous membrane. The occurrence of these pathological changes was significantly higher in female animals than in males: 11 out of 14 female rabbits and 4 among 11 male rabbits exhibited histological changes. Immunoprecipitation using gel-diffusion and histological analysis by an immunofluorescence technique showed that this antigen was localized exclusively in the bile duct but not in either hepatocytes or other tissues. It is suggested that histological changes induced following immunization with bile duct antigen are specific immunological phenomena. This interesting experimental model may be helpful in explaining the pathogenesis of primary biliary cirrhosis.

Animals↗

PubMed1980

Funicular orientation by electrical stimulation and internal neurolysis in peripheral nerve suture.

Eleven peripheral nerve lacerations around the wrists of ten patients were treated with funicular suture or nerve graft. In three freshly lacerated nerves funicular orientation could be made only by electrical stimulation to both cut ends. The electrophysiological method was also utilised to obtain funicular orientation of a proximal stump in eight old nerve lacerations. However, funicular orientation of the distal stump of old lacerations, which was not responsive to electrical stimulation, was performed anatomically by internal neurolysis from a terminal branching area up to a distal stump. By six months after the operation, motor and sensory functions of the patients with funicular suture had recovered to an excellent degree with rapid reinnervation.

Adolescent↗

PubMed1980

The contribution of computed tomography in the choice of an anterolateral approach, for treating cervical dumb-bell tumours.

Two cases of cervical dumb-bell tumour were operated on through the eroded and enlarged intervertebral foramina after the exact position of the tumours had been revealed by CT scan. This anterolateral approach was probably the best route for minimizing any operative damage to the spinal cord in these very advanced cases.

Adult↗

PubMed1980

Influence of After-ripening on Phytochrome Control of Seed Germination in Two Varieties of Lettuce (Lactuca sativa L.).

The reversible photoreaction on seed germination of two varieties of lettuce differs remarkably not only with the variety but also with the germination temperature and the physiological conditions of seeds caused by after-ripening.Grand Rapids showed reversible photoreaction at 25 C with 0 to 6 months and at 30 C with 5 to 6 months after-ripening. MSU 16 did not show any reversible photoreaction at 30 C with 0 to 6 months or at 25 C with 0 to 3 months after-ripening although they were completely reversible with 4 to 6 months after-ripening.These two varieties of lettuce seeds, however, showed reversible photoreaction at 20 C when they were sown immediately after harvest or after 1 and 2 months after-ripening. The photoreversion decreased after the 4-to 6-month stage.

Journal Article↗

PubMed1980