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Biomedical subjects

T Matsuba

Publications and source records attributed to T Matsuba.

At least 55 records · Page 3Linked to original sources

Induction of anti-Theileria sergenti antibodies in calves with murine monoclonal anti-idiotype antibody.

Anti-idiotype (anti-Id) antibody that contains an internal image of Theileria sergenti (T. sergenti) merozoite surface antigen was intramuscularly inoculated to calves to induce anti-T. sergenti antibody. In immunized calves, anti-anti-Id antibodies were induced and these antibodies bound to merozoites of T. sergenti were confirmed by immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA). In this experiment, one calf with the highest antibody titer against anti-Id antibody was intravenously challenged with T. sergenti-infected erythrocytes to show insufficient protective effects of the anti-anti-Id antibodies against the challenge.

Animals↗

Configuration of the active Mg-ATP complex in protein kinase C reaction.

To probe the active site structure of protein kinase C stereochemical studies were carried out by using ATP beta S. The enzyme utilizes either one of the diastereomers (SP and RP) of ATP beta S almost equally well as a substrate. This result contrasts with that for cyclic AMP-dependent protein kinase, suggesting that the topography of the nucleotide-binding site is significantly different between the two kinases.

Adenosine Triphosphate↗