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Biomedical subjects

T Manabe

Publications and source records attributed to T Manabe.

At least 559 records · Page 31Linked to original sources
PubMed1977

Molecular weight estimation of bovine brain mitochondrial monoamine oxidase.

The molecular size of bovine brain mitochondrial monoamine oxidase (MAO) was investigated Mitochondria were solubilized with an anionic detergent. Emarl 20C, and fractionated by ammonium sulfate. Ammonium sulfate-fractionated MAO was subjected to detergent-containing gel chromatography and detergent-containing gel electrophoresis. MAO activity appeared as single symmetrical peak in gel chromatography in the presence of 1% Emarl 20C, and the molecular weight was estimated to be 44,000. Polymerization of MAO was observed when gel chromatography was performed in lower (0.1%, 0%) concentrations of Emarl 20C. Activity staining of MAO after electrophoresis on a gel containing 0.1% Emar 20C was successful. The molecular weight of MAO estimated from the mobility of this stained band was 89,000. It is suggested that the molecular weight of MAO is 44,000 and that it recombines in low concentrations of the detergent to form complex particles with molecular weights of 89,000 or more.

Animals↗
PubMed1977

[The combination of STA-MCA anastomosis with another operation for the occlusive cerebrovascular disease (author's transl)].

Case 1. A 65 year old male had left hemiparesis with sudden onset since 8 years ago, which gradually aggravated for these 2 years. On Sept. 27, 1973, he was admitted to the Department of Neurosurgery, Kitano Hospital. There was left spastic hemiparesis with hemisensory disturbance and he could not walk without help for the maked spasticity. Left carotid angiogram revealed the complete occlusion of the internal carotid artery and marked stenosis of the external carotid artery at the common carotid bifurcation. External carotid endarterectomy was performed on Nov. 19, 1973, which was followed by STA-MCA anastomosis 2 months later. The spasticity of extremities and left hemisparesis were gradually improved and he was able to walk without help. Case 2. On Apr. 14, 1974, a 63 year old female developed complete stroke with right hemiparesis and speech disturbance after transient ischemic attacks of 5 days duration. On Aug. 9, he was admitted and had emotional incontinence, right hemiparesis, Gerstmann's syndrome and motor aphasia. Left carotid angiogram revealed a saccular aneurysm of the middle cerebral artery and the occlusion of the distal middle cerebral arterys. These findings suggested that the occlusion was caused by embolus from the middle cerebral aneurysm, and the combined surgery with STA-MCA anastomosis and operation for the aneurysm was planned. On Aug. 30, 1974, under left frontotemporal craniotomy, aneurysmal neck clipping and aneurysmectomy were performed and thereafter, STA-MCA double anastomosis was done. One week after operation, the gradual improvement of pre-operative symptomes was noted. Recently, STA-MCA anatomosis is well known to be one of the effective operative methods for the occlusive methods for the occlusive cerebrovascular diseases and in addition, we found that the combination of STA-MCA anastomosis with other operations was effective for unusual cases presenting in this report. Furthermore, except for the occlusive cerebrovascular diseases, we usually plan STA-MCA anastomosis for the cases of 1) carotid ligation or trapping for carotid-cavernous sinus fistula and some internal carotid aneurysms, 2) some intracranial tumors with the danger involving the main cerebral arteries by operation to protect the cerebrovascular insufficiency.

Aged↗
PubMed1977

Changes of upper gastrointestinal blood flow after hemorrhage in rabbits.

The effect of hemorrhage and blood transfusion on the regional blood flow of the upper gastrointestinal tract was measured with radioactive microspheres in a rabbit shock model. Fifteen minutes after hemorrhage, mucosal blood flow in the esophagus, corpus, and antrum and total blood flow in the duodenum decreased drastically. The persistent reduction of mucosal blood flow continued until 60 minutes after hemorrhage. In spite of these distribution patterns of mucosal blood flow during hemorrhage, no macroscopic change was seen in any part of the gastrointestinal mucosa. One hour after blood reinfusion, the mucosal blood flow in the corpus was increased markedly, and innumerable hemorrhagic erosions appeared in this region. It may be that the striking increase of mucosal blood flow due to blood transfusion caused bleeding from the disrupted mucosa of the corpus. At this time the impaired flow in the esophagus, antrum, and duodenum tended to be improved already. Six hours after blood reinfusion, the bleeding from the mucosal foci in the corpus ceased and the mucosal blood flow returned to almost normal. Thus the higher susceptibility of the corpus to hemorrhagic shock may be due to the greater degree of rapid increase of mucosal blood flow occurring in this portion alone after blood transfusion as well as to its higher vulnerability to ischemia in the hemorrhagic period. The flow patterns in the muscular layer almost paralleled those in the mucosal layer during hemorrhage and blood transfusion.

Animals↗
PubMed1976

Chemical reactivity of labile sulfur of iron-sulfur proteins. The reaction of triphenyl phosphine.

The reaction of triphenyl phosphine to iron-sulfur proteins from adrenal cortex mitochondria, spinach chloroplasts, and Clostridium pasteurianum was investigated. As ethanol concentrations in the reaction mixture increased, the rate of the reaction decreased. In the simultaneous presence of 1 M KC1 and 5 M urea, the reaction rate reached at maximum. Under these conditions the initial rates of the decolorization reaction by the phosphine were found to be 8.7, 0.88, and 1.8 nmol of ferredoxin per min at 25 degrees C for adrenal, spinach, and clostridial ferredoxins, respectively. The kinetic curves for the reaction of the phosphine sulfide formation, the loss of labile sulfur, and the deterioriation of visible absorption showed a similar pattern with a comparable rate. During this reaction, the complete reduction of ferric ions present in ferredoxin was observed with a fast rate under either aerobic or anaerobic conditions. These results suggest that the iron atoms in ferredoxin are first reduced by the intramolecular reductants in the presence of triphenyl phosphine with the concomitant formation of S2-2, which then reacts with triphenyl phosphine resulting in the formation of triphenyl phosphine sulfide.

Adrenal Cortex↗
PubMed1976

Kaposi's sarcoma of scrotum.

A rare case is presented of Kaposi's sarcoma localized to the scrotum with history, and physical and laboratory findings. Excision of the scrotum with bilateral orchiectomy was performed, with uneventful recovery. Literature is reviewed of Kaposi's sarcoma originating in the male external genitalia.

Aged↗
PubMed1976

[Dermoid tumor in left cerebellum--A case report (author's transl)].

This paper describes a dermoid tumor which developed in the dorso-lateral area of the left cerebellar hemisphere. It is the first to be reported in Japan. The patient was a 31 year old female who suffered from severe occipital headaches, frequent vomiting and gait disturbances. Neurological findings on admission included marked bilateral choked discs, horizontal nystagmus on gaze to the left, and poor coordination on the finger to nose test, nose-finger-nose test and and heel-shin test on the left side. Left suboccipital craniectomy was performed. A tumor was found which displaced the left cerebellum anteromedially and inferiorly. The postero-lateral surface of the tumor was adherent to the overlying dura matter. It was completely removed and found to be about the size of a hen's egg. It contained a yellow, porridge-like substance and short hairs. Histological examination of the capsule of the tumor showed stratified squamous epithelium and cutaneous structures, such as hair follicles and sebaceous glands. The diagnosis was dermoid tumors. We could find as many as 78 cases of the posterior fossa dermoids in the literature. In 72 cases (92.4%), the mass existed along the midline in the posterior fossa. The dermoids developed in the cerebellar hemisphere were reported in only 6 cases (7.6%) including the present case. Laterally growing dermoids are considered to be very rare. The pathogenesis is discussed and the literature reviewed.

Adult↗
PubMed1976

[Spinal cord arteriovenous malformation during pregnancy (author's transl)].

On May 7, 1973, a 23-year-old female, para ii, gravida ii, suddenly developed severe chest pain at 10th month of her pregnancy and soon after became paraplegic with sensory impairment lower than the level of 5th thoracic nerve and urinary incontinence. On May 8, she was admitted to our clinic and 4 days after, labour was induced by Caesarean section. Myodil myelography demonstrated "worm like defects" at the level of Th4-Th5, but sabarachnoid block was not found. Selective spinal angiography showed the image of typical arteriovenous malformation at the level of Th2-Th6. On June 26, about 1.5 months after delivery total removal of arteriovenous malformation (from Th2 to Th6)) was performed by the use of operative microscope. 10 months after operation, the patient could walk with her baby in her arms and no urinary incontinence was seen. We discussed the clinical characteristics and the treatment of spinal cord arteriovenous malformation during pregnancy.

Adult↗