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Biomedical subjects

T Majima

Publications and source records attributed to T Majima.

At least 127 records · Page 7Linked to original sources
PubMed1983

Immunoassessment of patients with gastric cancer. Using leukocyte migration inhibition (LMI) test.

Leukocytes from patients with gastric, colorectal or benign gastrointestinal disease, as well as those from normal control subjects, were tested by the leukocyte migration inhibition test (LMIT), using five different allogeneic 3 M KCl extracts of gastric cancer extracts. In LMIT with a single tumor extract, a pathological MI was found in 48% of 79 gastric cancer patients, a significantly higher figure than in the three other groups of patients (4-21%). In the panel mode of LMIT, that is, testing each blood sample with five different tumor extracts, 62/79 (79%) of patients with gastric cancer showed a positive reaction. Positive reactivity was significantly higher in patients with gastric cancer than in patients in the other groups and the frequency was higher in stage IV cases than in stage I-III cases, although the differences were not significant. In addition, the frequency of the positive reaction was unrelated to the degree of differentiation in adenocarcinoma. Positive reactivity increased in inverse proportion to the degree of nuclear grade (NG) (84% in NG I, 80% in NG II and 64% in NG III), but the correlation was not statistically significant.

Adult↗
PubMed1983

Studies on effect of carcinoembryonic antigen on leucocyte migration inhibition test in patients with colorectal cancer.

Leucocyte migration inhibition test (LMIT) is a useful method to detect tumor associated antigens (TAA) in cancer patients. Carcinoembryonic antigen (CEA) is one of the best tumor markers for gastrointestinal cancer, and especially for colorectal cancer patients, who show high plasma CEA level frequently. In this study, we performed LMIT in 71 colorectal cancer patients with 3 M KCl extracts of cancer tissues and measured concentration of CEA in the plasma and the extracts of cancer tissue simultaneously. Although CEA in colorectal cancer extracts was individually varied from low to high in concentration, the levels of CEA had no relation to LMI reactivity. In addition, the LMI reactivity of colorectal cancer patients did not relate to the plasma CEA level of the corresponding patient. The results suggest that some antigens which induce leucocyte migration inhibition factor (LMIF) to lymphocytes from colorectal cancer patients might be different substances from CEA.

Carcinoembryonic Antigen↗
PubMed1980

Membrane potential fluctuation in Paramecium.

The fluctuation of the membrane potential in Paramecium caudatum cells was measured. The fluctuation was very large and composed of slow and periodic components and fast and random ones. The power of fast fluctuation depended on the environmental salt condition, particularly on the ratio of the potassium ion concentration to the square root of the calcium ion concentration, [K+]o/[Ca2+]o(1/2), in the medium. The power greatly increased with the decrease of this ratio. The power density spectrum of fluctuation was of the 1/(1 + (f/fc)2) type when the total power was large. There, the fluctuation arose mainly from the local current of potassium ions. Some cooperation among potassium channels to increase the fluctuation was suggested. The spectrum became of the 1/f type when the total power was small.

Journal Article↗
PubMed1980

[Comparative study on rat arthritis induced by various immune mechanisms (author's transl)].

Experimental arthritis was induced in rats by the use of horse ferritin as an antigen. The pathological findings were studied and the mechanisms of inflammation were analyzed. Arthritis was induced through various mode of actions: 1. Arthus reaction (antigen-induced arthritis). 2. Passive Arthus reaction. 3. Reverse passive Arthus reaction. 4. Arthritis induced by intra-articular injection of immune complex, by either single shot or repeated injections. 5. Arthritis induced by anamnestic reaction. Arthritis induced by repeated injections of immune complex or that induced by anamnestic reaction exhibited mild chronic synovitis characterized by round cell infiltration without detectable acute phase of inflammation. Arthritis induced through other immunological mechanisms revealed a sequential course of acute synovitis characterized by accumulation of polymorphonuclear leucocytes (PMN) followed by gradual disappearance of PMN and alternative increase of round cells or plasma cells and proliferation of synovial lining cells. All types of arthritis subsided with mild fibrosis of the synovial tissue without lasting over a significantly long time. Although the successful production of a long-lasting chronic arthritis has been reported in rabbits, it was impossible to induce such long standing arthritis through this experiment. The chronicity of the arthritis induced through immunological mechanism may not simply be due to the long-standing retention of immune complex within the involved joint but also due to other factors such as the species of the test animals, the nature of antigen used and local factors produced through acute joint inflammation.

Animals↗
PubMed1978

Demonstration of antibody for glutamic pyruvic transaminase (GPT) in chronic hepatic disorders.

The present paper describes the detection of an autoantibody for glutamic pyruvic transaminase (GPT) in sera of patients with chronic hepatic disorders. In 16 out of 500 patients, the existence of an antibody for pig GPT was demonstrated by the double antibody method, gel filtration and radioimmunoelectrophoresis. The antibody was demonstrated as an immunoglobulin G (IgG) with either polyclonal or monoclonal type (kappa or lambda). The binding portion of IgG with GPT was determined as the fragment Fab, but not Fc of IgG. Because the binding of 125I-pig GPT with the patient's antibody was displaced by human GPT, this antibody may have the characteristic of cross reacting with both pig and human GPT. Although the mechanism of production of the antibody for GPT and the pathological significance of the antibody in chronic hepatic disorders remained obscure, possible inhibition of GPT activity in serum is suggested in the presence of this antibody.

Alanine Transaminase↗
PubMed1976

Determination of triiodothyronine in red blood cells by radioimmunoassay.

This study was undertaken to determine T3 content in red cells by radioimmunoassay. T3 in red blood cells was solubilized fairly from the stroma by hemolysis and red-cell T3 content could be determined directly by radioimmunoassay of the lysate. After hemolysing red cells with an equal volume of distilled water, 0.4 ml of the hemolyzate was used for the assay. The red-cell T3 content was expressed as ng/ml of red-cell volume. The normal T3 range in red cells was 0.20-0.45 ng/ml, and the Mean+/-SD was 0.32+/-0.10 ng/ml. The limit of detectability was 0.2 ng/ml. In hyperthyroid patients, the red-cell T3 content was more than 0.50 ng/ml with a Mean +/-SD of 1.35+/-0.65 ng/ml. In hypothyroid patients, red cells contained less than 0.25 ng/ml of T3, and there was an overlap from 0.20 to 0.25 ng/ml in the content of red-cell T3 in hypothyroid and euthyroid subjects. The patients with T3 toxicosis showed a high or normal level of red-cell T3. A positive correlation was noted between the red-cell T3 content and the serum T3 level (r=0.66). The correlation between the red-cell T3 content and the free T4 index (expressed as T7) was also positive (r=0.67). From these experiments, it is suggested that the red-cell T3 is low in comparison with the serum T3 levels, and depends on two factors; serum T4 and serum T3 levels.

Binding, Competitive↗
PubMed1975

Radioimmunoassay for estimation of thyroglobulin in human serum.

A specific double antibody radioimmunoassay has been develop for the measurement of thyroglobulin in human serum. Human thyroglobulin was purified by combined DEAE-cellulose and affinity chromatography using Sepharose 4B-bound Concanavalin A. Sensitivity of test serum was 10 ng/ml. Thyroglobulin was not detectable in half of normal subjects, and half showed values between 10 and 180 ng/ml. In the patients with simple goiter and secondary hypothyroidism, serum thyroglobulin was usually in the normal range. In Hashimoto's thyroiditis, many sera having precipitating antibodies or high hemagglutination antibodies for thyroglobulin showed a high thyroglobulin concentration in serum probably due to a false positive reaction. In hyperthyroidism, an increased thyroglobulin level was observed in 64% of patients. However, there was no correlation between serum thyroglobulin and thyroxine levels in untreated hyperthyroidism. Serum thyroglobulin was increased significantly in some cases for several weeks after isotope therapy for the hyperthyroidism.

Goiter↗