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Biomedical subjects

T Maeda

Publications and source records attributed to T Maeda.

At least 937 records · Page 52Linked to original sources
PubMed1990

Expression of IgH promoter/enhancer Ly-1 transgene in hematopoietic chimeric mice generated by embryonic stem cell line.

We have produced hematopoietic chimeric mice from an embryonic stem (ES) cell line carring Ly-1 cDNA under the control of IgH promoter and enhancer. Various amounts of serum IgM (5-86% of total IgM) in chimeric mice were of ES origin and 30-60% of IgM-positive B cells from the chimeric mice analyzed were reconstituted from ES cells. Using these chimeric mice, the expression of the Ly-1 transgene on lymphoid tissues was examined by polymerase chain reaction assay with primers specific for the transgene, and by cell sorter analysis. Transcription of the Ly-1 transgene was detected in spleen cells, thymocytes and lymph node cells; however, the expression of the Ly-1 molecule was observed only on lipopolysaccharide (LPS)-stimulated splenic IgM-positive B cells but not on resting splenic B cells. There was no significantly increased expression of Ly-1 on splenic T cells and thymocytes. Thus, our findings demonstrate that conventional splenic B cells could express the Ly-1 transgene on their surface in vivo after LPS stimulation. Also discussed is the ES-derived chimeric hematopoietic system.

Animals↗
PubMed1990

[Effect of shakuyaku-extract on electrical stimulation induced contraction of guinea pig ileum].

It was reported that Paeoniae Radix extracts (S) depressed the contraction induced by electrical stimulation on the isolated guinea pig ileum. In the present study, the mechanisms of the inhibitory action of S on the contraction of guinea pig ileum were investigated. Theophylline and phentolamine are respective antagonists of adenosine and norepinephrine, decreased the inhibitory action by S on the contraction of guinea pig ileum about 50%. Thus these data suggest that some unknown substances with adrenergic and adenosine like actions may be contained in S. Furthermore the possibility of other inhibitors in S was suggested.

Adenosine↗
PubMed1990

[Zinc and copper concentrations in breast milk and maternal serum in the postpartum period].

This study reports the contents of Zn and Cu in the breast milk and serum of postpartum mothers, 17 primiparas and 20 multiparas, at one week and at one month after delivery. Results were as follows. 1. The mean content of Zn in the breast milk was 5.44 micrograms/ml at 1 week after delivery, and it decreased significantly (p less than 0.01) to 2.73 micrograms/ml at 1 month after delivery. 2. The mean content of Zn in serum was 0.66 microgram/ml at 1 week, and it increased significantly (p less than 0.01) to 0.84 microgram/ml, close to the normal level, at 1 month. 3. The milk Zn level at 1 week after delivery was about 8 times as high as the Zn in serum. There was a significant (p less than 0.05) negative correlation in Zn contents between milk and serum at 1 week after delivery, and there was no significant correlation in Zn contents between milk and serum at 1 month after delivery. 4. The mean content of Cu in breast milk was 0.55 microgram/ml at 1 week after delivery, and it decreased to 0.44 microgram/ml at 1 month after delivery. 5. The mean content of Cu in serum was 2.14 micrograms/ml at 1 week after delivery, and it decreased significantly (p less than 0.01) to 1.35 micrograms/ml, close to the normal level, at 1 month after delivery. 6. Concerning the Cu contents of milk and serum, there was not a significant correlation at 1 week after delivery, but a significant (p less than 0.05) positive correlation was found at 1 month after delivery.

Adult↗
PubMed1990

[Silicon concentrations in maternal serum and breast milk in the postpartum period].

The silicon (Si) concentrations in maternal serum and breast milk were determined for 38 healthy postpartal women, 19 primiparae and 19 multiparae, by using inductively coupled plasma-atomic emission spectrometry (ICP-AES). The results are as follows: 1. The geometric mean (95% confidence interval) of the Si level in maternal serum was 9.7 mumol/l (8.8-10.8 mumol/l) at 1 week after delivery, and 8.1 mumol/l (7.1-9.2 mumol/l) at 1 month after delivery. The Si level at 1 week after delivery was significantly (p less than 0.05) higher than that at 1 month after delivery. 2. The geometric mean of the Si level (95% confidence interval), in breast milk was 6.3 mumol/l (5.4-7.3 mumol/l) at 1 week after delivery, and 6.1 mumol/l (5.1-7.2 mumol/l) at 1 month after delivery. 3. At both 1 week and 1 month after delivery, a significant correlation was not found between the Si concentrations in maternal serum and in breast milk.

Adult↗
PubMed1990

Nerve terminals in human periodontal ligament as demonstrated by immunohistochemistry for neurofilament protein (NFP) and S-100 protein.

Nerve terminals in the human periodontal ligament were investigated by immunohistochemistry for neurofilament protein (NFP) and glia-specific S-100 protein. The human periodontal ligament was found to be innervated by NFP-immunoreactive nerve fibers, and contained free and specialized nerve endings; essentially all these nerve fibers were associated with S-100 protein-immunoreactive elements. Free nerve endings showing tree-like ramifications were frequently observed along the whole length of the tooth. The specialized nerve endings consisting of thick nervous elements were classified into four types as follows: Ruffini-like endings which were composed of expanded nerve terminals arranged in a dendritic fashion were found mainly around the root apex; coiled nerve endings were located in the mid-region of the periodontal ligament; spindle-shaped types, and expanded nerve endings, both rarely found near the root apex. Immunostaining for S-100 protein showed that no lamellated nerve terminals were found in the human periodontal ligament. The region-specific distribution of various nerve terminals demonstrated in this study seems to be suited to effectively receive mechanical stimuli to the teeth from various directions.

Humans↗
PubMed1990

[Clinicopathological study on low grade glioma. In relation to malignant transformation].

The clinical status of patients with glioma is influenced by 1) the histological malignancy of the tumor, 2) the tumor volume, 3) secondary status such as brain edema or intracranial hypertension due to the tumor, and 4) the host immunity. Due to some improvement in at least 2) and 3) by the initial treatment, most low grade glioma cases pass through a clinically silent postsurgical period. However, at a certain point, transition to a high grade tumor malignant transformation may occur with exacerbation of the symptoms. Twenty-two cases of histologically established low grade glioma experienced over the past 7 years, in which immunological status was evaluated, were analyzed. Nine cases (41%) showed malignant transformation. Characteristic pictures of the clinical symptoms, computed tomography (CT) scan findings, immunological status, and morphological findings (mainly immunohistochemical examination) in nine cases were delineated. The findings at the time of exacerbation of the symptoms were as follows. In all cases CT scan demonstrated the change in the main lesion from low density to mixed density and were compatible with a high grade glioma. Reduction in host immunity was verified. Morphological increase in the tumor volume, increase in histological malignancy and deterioration in the secondary status due to the tumor were confirmed. Necrosis of the tumor cells as well as increase in giant cells and gemistocytes were observed. Immunohistochemical analysis revealed a decrease and irregularity in glial fibrillary acidic protein positive cells and positive processes as well as increase in vimentin intensity. These findings demonstrate change in the biological characteristics of the tumor.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1990

[Brain metastasis from primary pericardial mesothelioma. Case report].

A 50-year-old female was admitted because of nausea, vomiting, and cerebellar ataxia. Computed tomography scan revealed an enhanced mass accompanied with a cyst in the right cerebellar hemisphere. The mass situated in the subcortical region was removed. Histologically, highly vascular tumor cells lined the cavities. Postoperative radio- and chemotherapy were administered and the clinical symptoms improved gradually. Two months later, the patient complained of dyspnea. Chest X-ray on second admission demonstrated cardiomegaly. Hemorrhagic pericardial effusion amounting to 1000 ml was aspirated by pericardial puncture. Papillary clusters of tumor cells were demonstrated in the pericardial effusion. The patient died of cardiac failure. At necropsy solid tumors were located in the heart, lung, left inguinal region, and cerebellum. Histological diagnosis was mesothelioma arising from the heart. Primary pericardial mesotheliomas are rare; approximately 106 cases have been reported. Pericardial mesothelioma frequently spreads to the adjacent pleura and mediastinum, but distant metastases are extremely rare because patients with pericardial mesothelioma tend to die early due to cardiac failure or cardiac tamponade.

Brain Neoplasms↗
PubMed1990

[Congenital anomalies of the coronary arteries with associated ischemic ST depression on exercise: a report of three cases].

This report presented evidence of myocardial ischemia as the etiology of angina pectoris in three patients with congenital anomalies of the coronary arteries but without arteriosclerotic disease. All of three cases showed angina pectoris and ST depressions on their exercise electrocardiogram. Case 1: This 58-year-old man developed angina pectoris at the age of 50 years. His treadmill exercise test precipitated chest pain and ST depression. His coronary arteriograms disclosed an ectopic origin of the right coronary artery just anterior to the origin of the left coronary artery in the left coronary sinus. No significant atherosclerotic stenosis was present. An apparent ischemic manifestation appeared to be caused by compression of an aberrant right coronary artery between the aorta and the right ventricular infundibulum. Case 2: A 49-year-old woman had a history of angina. Her treadmill exercise test induced chest pain and an abnormal exercise electrocardiographic finding. Her coronary arteriograms revealed a single left coronary artery. Insufficient perfusion was postulated as a cause of apparent myocardial ischemia in this case though angiographically, there was adequate perfusion. Case 3: This 31-year-old man had a six-year history of angina. His treadmill exercise electrocardiograms revealed ischemic changes accompanied by chest pain. Coronary arteriograms disclosed a coronary artery fistula. The ischemic manifestation was apparently caused by inadequate perfusion due to coronary steal. With the increasing use of coronary arteriography, unusual origins and courses of coronary arteries will be more frequently encountered. Precise knowledge of anomalies is prerequisite for evaluating variations in the location of the coronary artery ostia and their statistical probabilities.

Adult↗
PubMed1990

[Improvement in the effects of radiation therapy with BRM].

The effects of radiation therapy and Sonifilan (SPG; a glucan produced by Schizophyllum commune) on the control of the primary lesion of human uterine cervix cancer and the experimental metastasis with mice were studied. SPG had excellent effects on the uterine cervix cancer compared with radiation therapy alone. Furthermore, immunohistochemical study showed that tumor infiltrated monocytes in good responders were mainly occupied with helper/induced T lymphocytes. SPG also suppressed the experimental lung metastasis especially with radiation therapy. Metastatic tumor infiltrated monocytes were many macrophages, which was the same as in inoculated local tumor.

Adenocarcinoma↗
PubMed1990

A transition space maintainer for the adolescent dentition: a case report.

Premature loss of teeth in posterior segments of the primary, mixed or permanent dentition may lead to space loss in an anterior-posterior dimension. This article illustrates how a simple, aesthetic and effective space maintainer can be used to prevent space loss in the permanent dentition.

Adolescent↗
PubMed1990

[An immunohistochemical study on the response of nerve fibers in the periodontium of rat molars during experimental tooth movement].

It is well known that orthodontic forces induce pain during tooth movement. However there have been few neurohistological studies on sensational periodontal tissue response to orthodontic tooth movement. So we have many unknown problems about reactions of periodontal nerve fibers during tooth movement. The present study deals with the response of nerve fibers in periodontal ligament of rat molar during experimental tooth movement by means of immunohistochemical method for calcitonin gene-related peptide (CGRP). The results obtained in this study were as follows: 1. In control sections, CGRP-immunopositive nerve fibers were observed both around the root apex and in the intermediate region of the periodontal ligament. Although most of those fibers was distributed around the blood vessels, a few nerves were recognized to terminate in the periodontal fibers. 2. At three days after tooth movement, the CGRP-positive nerves gradually increased in number around the blood vessels. After that, the number of CGRP-positive nerves decreased and no difference of distribution of CGRP-positive nerves was observed between the control sections and the experimental sections at seven days. 3. Especially, at three days when active remodeling of alveolar bone was taking place, a few nerve fibers showing CGRP-immunoreactivity were observed to concentrate around osteoclasts or to migrate Howship's lacunae. These findings showed the dynamic changes of CGRP-immunoreactive nerves in the periodontal ligament during tooth movement, and suggested that this reaction of the periodontal nerves might induce pain in patients. In addition to neurotransmitter function, it was supposed that CGRP directly or indirectly effected the cellular elements and surrounding tissues on peripheral organs such as vascular system and osteoclasts.

Animals↗
PubMed1990

[The management of drooling in patients with cerebral palsy--submandibular duct relocation].

Drooling is a significant problem demonstrated by individuals of all ages with a variety of etiologies such as neurological disorders, cerebral palsy, mental retardation and others. Persistent drooling not only creates troublesome hygienic problems for patients, teachers, nurses, and playmates because of the constant soiling of clothes, toys, and work materials, but also causes an odor from their clothing and bibs. The older patient with normal cognition is disturbed by the drooling and may become depressed and reclusive. Successful management of drooling alleviates these problems, improves appearance and self-esteem and significantly reduces the time involved in the care of the sufferers. The author, who had studied at the University of Toronto for 2 years, acquired the technique of submandibular duct relocation under the guidance of Dr. Crysdale. We carried out the surgical procedure of submandibular duct relocation for drooling on six patients who had cerebral palsy. The surgical procedure resulted in a dramatic decrease in drooling and odor levels. All of them improved in appearance, and the time involved in the care of the suffers was significantly reduced. The complication of ranula, however, appeared in two cases, which suggested a much higher frequency than that in the report of Crysdale. It seems that a detailed explanation to parents and teachers about the advantages and disadvantages of submandibular duct relocation is important.

Cerebral Palsy↗
PubMed1990

[The mean of Downs and northwestern analysis in adults].

An angle analysis and linear analysis are popularly used in clinical orthodontics. Downs analysis and Northwestern analysis are two methods of angle analysis, and the mean values reported by Iizuka in 1963 are still used. However in Japan each generation's constitution (height, weight) tends to increase year by year. For example, the mean height and weight of adult males (23 years old) have increased by 5.0cm and 7.6kg as compared with nearly 20 years ago. Therefore the mean of 20 years ago may not be appropriate. Analytical values at normal occlusion Japanese adults by Kayukawa, Iizuka, Ishikawa, Yamauchi, Miura, Iwazawa and, most recently by Asai in 1973. We obtained these values and determined the validity of currently used values.

Adult↗
PubMed1990

Estimation of the gonial angle from the orthopantomogram.

We set a new basic plane on an orthopantomogram in order to measure the gonial angle and obtained the following: 1) Usable error difference in ordinary clinical setting ranged from 0.5 degrees-1.0 degree. 2) Because there is no great difference between the levels of the gonial on orthopantomograms, they are effective for investigation of the changes of side and degree. Thus, based on the above results, 100 cases were randomly selected from the Angle class III cases which were treated at our department of orthodontics in order to investigate the differences between the left and the right side and pre and post-treatment changes and obtained the following results: 1) Pretreatment differences between the right and the left sides were recognized in 88 cases. 2) 66 cases showed posttreatment improvement in the differences between both sides. Because orthopantomograms are applied at the end of treatment and for evaluation of the stability of occlusion. We will investigate if the occlusion is best harmonized at equal gonial angles on both sides, using a combination of EMG and measurement of occlusion force, and will also study various cases in more detail.

Cephalometry↗
PubMed1990

[A case of Fabry's disease detected by renal biopsy findings].

A 39 year-old man was found to have mild proteinuria by urinary examination since one year ago. He was for the first time diagnosed as having Fabry's disease by histopathological and electronmicroscopic findings of the renal biopsy specimens, which showed the presence of numerous vacuolated cells and electron dense bodies inside the cells. The level of WBC alpha-galactosidase was significantly lower than normal level. The pedigree of this patient showed a familial history of various types of renal disease. One of the patient's brothers also showed decreased level of WBC alpha-galactosidase, who has been treated by maintenance hemodialysis for 2 years. It is concluded that early diagnosis of this disease through renal biopsy and WBC alpha-galactosidase level is important to manage the future course of patients with Fabry's disease.

Adult↗
PubMed1990

Somatostatin receptors on human lymphocytes and leukaemia cells.

Receptors for somatostatin were identified on mitogen-activated human peripheral blood lymphocytes (PBL) and human leukaemic cells in 87.5% of lymphoblastic leukaemia and in 12.5% of non-lymphocytic leukaemia, using a somatostatin radiobinding assay. The specific binding of 125I-somatostatin of these cells increased linearly with the cell numbers and was suppressed by non-iodinated somatostatin. We investigated the distribution of fluorescent somatostatin to mitogen-activated PBL by using a fluorescence-activated cell sorter (FACS). Over 95% of the cell populations bound fluorescent somatostatin and no distinct predilection was found among certain lymphocyte subpopulations and somatostatin receptor-positive cells. Scatchard analysis showed a single class (low affinity) of binding site on mitogen-activated PBL and two classes (high and low affinity) of specific binding sites on lymphoblastic leukaemia cells.

Cell Separation↗
PubMed1990

[Treatment of acute lymphoblastic leukemia in adults by the modified protocol of L-10M (Sloan-Kettering)].

We have treated 20 adult patients with acute lymphoblastic leukemia (ALL) and 2 patients with lymphoblastic lymphoma with a protocol modified from L-10M of the Memorial Sloan Kettering Cancer Center. Eighteen patients (81.8%) entered complete remission (CR). Eight of them eventually relapsed (only 1 patient had a meningeal relapse) and died. Median CR duration was 19 months (median overall follow-up of 35 months and 50% remission duration was not yet determined. Median overall survival was 19 months. Three patients died of sepsis during remission induction, but all of other deaths were due to resistant or relapsed leukemia. The four patients who completed 3.5 year's modified L-10M protocol were free from relapse for 6-11 months (mean 8.7). Although further follow-up is necessary, we suggest that modified L-10M protocol is effective for adult ALL and long-term survival may be available.

Adolescent↗