Biomedical subjects

T Machii

Publications and source records attributed to T Machii.

87 records · Page 5Linked to original sources

PubMed1984

Similarities between IgG-bearing lymphocytes and hairy cells: cytologic and cytochemical studies.

Six patients with hairy cell leukemia (HCL) were studied for surface immunoglobulin ( sIg ). In all five sIg -positive cases, the heavy chain isotype was IgG. We performed cytologic and cytochemical studies of sIgG + lymphocytes in normal peripheral blood and compared them with hairy cells. Normal sIgM + lymphocytes were also examined. sIgG + and sIgM + lymphocytes made up 0.9% and 6.1% of normal peripheral blood lymphocytes, respectively. Under a phase-contrast microscope, 76% of sIgG + lymphocytes showed cytoplasmic processes similar to those found on hairy cells, whereas most sIgM + lymphocytes had smooth surfaces. Tartrate-resistant acid phosphatase (TRAP) staining revealed that TRAP-positive cells accounted for 65% of sIgG + lymphocytes and 19% of sIgM + lymphocytes. Some (8.3%) of the sIgM + lymphocytes expressed sIgG concomitantly. When sIgM +, sIgM +, sIgG + lymphocytes were excluded, the percentages of cells with surface processes and of TRAP-positive cells in the remaining sIgM +, sIgG - lymphocytes were 10% and 12%, respectively. A very small proportion (0.2%) of sIgM -, sIgG - lymphocytes had cytoplasmic processes. These results indicate that normal sIgG + lymphocytes are cytologically and cytochemically different from most sIgM + lymphocytes and that the phase-contrast microscopic appearances and TRAP activity of sIgG + lymphocytes are similar to those of HCL tumor cells.

Acid Phosphatase↗

PubMed1983

A case of T-cell chronic lymphocytic leukemia (T-CLL) expressing a peculiar phenotype (E+, OKM1+, Leu 1+, OKT3- and IgG EA-).

A case of chronic leukemia is reported. Malignant cells had the morphology of lymphocytes and an affinity for skin. Cytochemically, they were peroxidase negative, and NaF-resistant alpha-naphthyl esterase positive. The peripheral mononuclear cells formed E-rosettes and reacted with Leu 1 and OKM1. They were unreactive with OKT3, OKT4, OKT6, OKT8, and OKIa1. The cells did not possess surface IG, C3b receptors, or IgG Fc receptors (EAIgG). They responded weakly to phytohemagglutinin, and did not respond to concanavalin A. The cells did not adhere to Petri dishes or phagocytose latex beads. It is concluded that it was a case of OKT3-, OKM1+, EAIgG- T-cell chronic lymphocytic leukemia. This suggests that OKT3 does not recognize all peripheral T-cells, and that OKM1 is not specific for the monocyte-myeloid lineage. These cells may be useful in the characterization of E+, OKM1+ subset of peripheral mononuclear cells.

Adult↗

PubMed1983

A human B lymphocyte antigen (P-76) shared by B-cell chronic lymphocytic leukemia cells and hairy cell leukemia cells.

A membrane antigen with an apparent specificity to B lymphocytes was detected with immunochemical techniques and its properties were analyzed. Anti-B-CLL serum was raised in a rabbit by immunization with B-cell chronic lymphocytic leukemia (B-CLL) cells. This anti-B-CLL serum was absorbed with erythrocytes, liver homogenate and insolubilized immunoglobulins. After further absorption with T-CLL cells, chronic myelocytic leukemia (CML) cells and acute myelocytic leukemia (AML) cells, the anti-B-CLL serum still reacted with peripheral blood B lymphocytes, B-CLL cells and hairy cell leukemia (HCL) cells. In contrast, no reactivity was seen with peripheral blood T lymphocyte or monocytes, or leukemia cells of non-B cell origin. An immunoprecipitation of radiolabeled cell surface proteins was attempted using the anti-B-CLL serum in the presence of Staphylococcus Aureus Cowan 1 (SaCl), and the precipitates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A membrane antigen with an apparent molecular weight of 76,000 daltons (P-76) was immunoprecipitated with the anti-B-CLL serum from the lysates of normal B lymphocyte, B-CLL cells and HCL cells. The antigen (P-76) is not composed of disulfide-linked subunits and has no structural relationship with HLA-DR (Ia-like) antigens or other known antigens. These results suggest that this antigen is B-lymphocyte specific, and favour the B-lymphocyte nature of HCL cells.

Antibody Specificity↗

PubMed1983

Ultrastructure of normal human T cell subpopulations. Parallel tubular arrays in T gamma lymphocytes and clustered dense bodies in T mu lymphocytes.

Ultrastructures of normal T-cell subpopulations, T gamma and T mu cells, were studied. T gamma cells were isolated and identified by repeating the rosetting method; firstly, by E rosette formation with neuraminidase-treated sheep red blood cells (SRBC), and next by EA gamma-rosette formation with ox red blood cells coated with IgG antibody (EAox). Before EAox rosetting, SRBC on isolated T cells were lysed by autologous plasma instead of ammonium chloride solution. Normal T gamma cells were heterogeneous with regard to their granules; the majority of T gamma cells had parallel tubular arrays (PTA) and a few had electron-dense granules. When ammonium chloride solution was employed to lyse SRBC, PTA were never observed; PTA in normal T gamma cells and in chronic lymphocytic leukemia cells with T gamma character both seemed to change into electron-dense granules after ammonium chloride treatment. In contrast to T gamma cells, T mu cells were characterized by clustered dense bodies, i.e. focal aggregates of electron-dense granules.

Ammonium Chloride↗

PubMed1983

Hairy cell leukemia: a report of 10 cases in Japan and characterization of anti-hairy cell sera.

A series of 10 cases of hairy cell leukemia (HCL) in native Japanese patients was studied. The clinicopathological features and the phenotype of the leukemic cells were compared with those of HCL in Western countries. The clinical pictures were similar to those in Western countries but some hematological findings were different. The Japanese patients had more marked leukocytosis, less granulocytopenia and thrombocytopenia, and no dry tap on bone marrow aspiration. The phenotype of hairy cells was much the same in both areas. Two kinds of anti-hairy cell sera were raised in rabbits. One (AHS 406) was obtained from rabbits immunized by injecting whole hairy cells. The other (alpha HC-G and alpha HC-M) was obtained by injection of membrane glycoproteins partially purified from hairy cells. The reactivity of the former antiserum (AHS 406) against leukemic cells from a variety of leukemias including HCL and B-cell chronic lymphocytic leukemia was studied by an immunoprecipitation technique. AHS 406 defined three cell surface antigens on HCL, P-30, P-35 and GP-135 (30,000, 35,000 and 135,000 daltons respectively). These three components are expressed only on neoplastic B lymphocytes. Quantitative differences in expression were observed in different types of leukemia, although all three antigens were most strongly expressed in HCL. alpha HC-G showed reactivity similar to that of OKIa-1. alpha HC-M reacted with hairy cells but not with other leukemic cells except mature granulocytes of chronic myelocytic leukemia. It also reacted with most granulocytes and a few mononuclear cells in normal peripheral blood. Preliminary immunochemical studies suggest that alpha HC-M reacts with a glycoprotein with a molecular weight of 26,000.

Adult↗

PubMed1982

Studies of human T gamma cells: division of a T gamma subset in normal and leukemic cells by using anti-T gamma-CLL heteroantiserum.

A specific heteroantiserum was prepared against the leukemic cells from a patient with T-derived chronic lymphocytic leukemia (T-CLL). The anti-serum was absorbed with cells of a morphologically different type from another patient with T-CLL. Both the immunizing cells and absorbing cells had Fc receptor for IgG (Fc gamma R), so the former case was named T gamma-CLL type 1, and the latter T gamma-CLL type 2. This antiserum, termed anti-T gamma-1, reacted with 19% of normal peripheral blood T lymphocytes, but not with non-T lymphocytes or monocytes. The T lymphocytes in the blood that reacted to anti-T gamma-1 were 72% of the T gamma cells. Anti-T gamma-1 also reacted to 60-78% of the thymocytes. Except for T gamma-CLL type 1 cells, anti-T gamma-1 did not react with various types of leukemia cells from lymphoid malignancies, myelogenous leukemias and monocytic leukemias. Studies on the relation between anti-T gamma-1 and OKT8 monoclonal antibody revealed that anti-T gamma-1 reactive (anti-T gamma-1+) cells and OKT8+ cells largely overlapped, but they were different in part. More interestingly, OKT8 inhibited Fc gamma R binding, but anti-T gamma-1 did not. These results indicate that anti-T gamma-1 is useful for detecting a certain subset of T cells and for classifying lymphoproliferative disorders.

Antibodies, Monoclonal↗

PubMed1981