The effect of alterations in arterial carbon dioxide tensions on choroidal blood flow in rabbits.
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Biomedical subjects
Publications and source records attributed to T M Wilson.
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Four enzymes were separated from an extracellular preparation of Bacillus polymyxa by carboxymethylcellulose column chromatography. The pH optima were 8.3 to 8.5, 8.7 to 8.9, 9.2 to 9.4, and 9.5 to 9.6. All of the enzymes required calcium ion for maximum activity, whereas strontium ion was only partially effective in stimulating activity. Cobalt was the only other cation tested which was effective in two of the enzymes. The lyases seem to attack a calcium salt-bridged substrate. K(m) and V(m) data of the four enzymes on various oligomers are presented as well as paper chromatographic evidence of preferred sites of attack. All of the enzymes are endo-enzymes which, based upon their characteristics, were classed into two types.
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The fine structure of the M and C forms of seal poxvirus as observed in negative stained preparations in the electron microscope are described. The M form has a beaded surface and a serrated outline and particles averaged 312.3 mmicro, in length and 193.8 microns in width. The C form was not beaded, had a serrated periphery and averaged 352.8 mmicro in length and 196 mmicro in width. A short discussion of the morphology of seal poxvirus in relation to other poxviruses is presented.
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