Search PubMed⌕ Search

Biomedical subjects

T M Feltkamp-Vroom

Publications and source records attributed to T M Feltkamp-Vroom.

At least 19 recordsLinked to original sources
PubMed1983

Sural nerve immune deposits in polyneuropathy as a remote effect of malignancy.

Sural nerves from six patients with polyneuropathy as a remote accompaniment of malignancy were studied by light microscopy, immunofluorescence, electron microscopy, and immuno-electron microscopy. Sural nerves also were studied in twelve control patients with malignant diseases without peripheral neuropathy and in fifteen with neuropathy associated with nonmalignant conditions. Only nerves from the first group of six patients showed immune deposits of immunoglobulin M, C3, and C1q as granular or confluent layers in the inner laminae of the perineurium as well as in and around endoneural capillaries. The findings are similar to those in published studies of Waldenström's macroglobulinemia, chronic relapsing polyneuropathy, and neuropathy of monoclonal gammopathy and extend the list of conditions to include several forms of carcinoma and Hodgkin's disease.

Adult↗
PubMed1982

Pseudo-tumours, a clinical concept.

UNLABELLED: Space-filling lesions in the orbit can be divided into 3 large groups: 1. Acute and subacute inflammatory processes (bacterial, fungal, parasitic, foreign body). 2. Benign and malignant tumours, true new growths. 3. Pseudo-tumours. The conditions in group 3 are also space-filling lesions, but have the characteristics of a chronic, infiltrating, inflammatory process. In 11% of 340 consecutive patients in the Orbital Centre in Amsterdam this diagnosis was made. The group is far from uniform: the dominant cell type varies from one case to another. Usually lymphocytic cells are dominant; if these are arranged in follicles the lesion is called a pseudo-lymphoma. This condition can gradually leave the province of the pseudo-tumours and begin to show the characteristics of a malignant or non-Hodgkin lymphoma. Sometimes the inflammatory character of the lymphoid infiltration is dominant, as in myositis, sclerotenonitis and infiltrative Graves' ophthalmopathy; these conditions are clearly related to immunological processes. Attempts to classify all pseudo-tumours on a pathological basis have remained rather vague. CLINICAL PICTURE: rapid development (2-6 months); pain and oedema are present, but these are not the principal symptoms; secondary features are: disorders of motility, visual loss and other symptoms. Bilaterality usually indicates the presence of a systemic disease.

Connective Tissue Diseases↗
PubMed1982

T cell leukemia presenting as chronic polyarthritis.

T cell leukemia was detected in a woman who suffered from chronic polyarthritis. The peripheral blood leukocytes were increased in number and consisted of lymphocytes, 95% of which could be identified as T lymphocytes. T cell infiltration was found in the bone marrow, the synovial fluid, and tissue, and in nodules macroscopically resembling rheumatoid skin lesions. Further investigation of these cells by enzyme chemistry, immunohistochemistry, electron microscopy, and cytochemistry revealed that they had irregularly indented nuclei, no alpha-naphthyl acetate esterase activity, and only faint granular acid-phosphatase activity. The cells were negative for Ia-like antigen and surface immunoglobulin. Analysis of the cell surface glycopeptides showed the presence of abnormally enlarged carbohydrate structures. These data suggest that these leukemic T cells are a malignant equivalent of immature T cells.

Adult↗
PubMed1981

A malignant tumor arising from interdigitating cells; light microscopical, ultrastructural, immuno-and enzyme-histochemical characteristics.

A tumor in a 37 years old male is described in which the tumor cells appeared to be derived from interdigitating cells normally found in the T-cell area of lymph nodes. The patient presented with superior vena caval obstruction due to a mediastinal mass, followed by lymph node enlargement and skin lesions leading to death within 4 months. The tumor cells lacked immune markers for lymphocytic cells. They showed Ia-like antigens and high adenosine triphosphatase activity, while acid phosphatase and alpha-naphthyl acetate esterase activity was absent. Their fine morphology was strikingly similar to that of interdigitating cells. A combination of these data led us to the conclusion that this tumor represents a specific subtype of the tumors derived from the mononuclear phagocyte system, namely a sarcoma of interdigitating cells.

Adult↗
PubMed1980

Non-Hodgkin's lymphoma. Immunohistochemical and electron microscopical findings in relation to lightmicroscopy. A study of 74 cases.

From 74 patients with non-Hodgkin's lymphoma (NHL), fresh biopsy material from involved tissue was investigated histochemically and by light and electron microscopy. The results were compared with the light microscopical conclusions based on three currently utilized classifications, namely of Rappaport, Lukes, and Lennert. The separate groups of low-grade NHL appeared to consist of homogeneous cell populations, both in immunohistological as well as in enzymehistochemical and electron microscopical sense. On the contrary, high-grade NHL constituted a heterogeneous group in which supplementary (immunohistochemical, enzymehistochemical, and electron microscopical) investigation is very useful. All NHLs reacted with anti-human lymphocyte serum, including the so-called histiocytic NHLs according to the Rappaport classification, which proves their lymphocytic origin. Consequently the prefix "histiocytic" should be altered in a morphogenetically correct way. Without exception the B-cell NHLs were characterized by the presence of monoclonal immunoglobulin on the cell membrane and/or intracytoplasmically. In a part of the immunologically non-T-/non-B-/("null cell") NHLs, a B-cell origin was indicated by the presence of ATPase and/or 5' nucleotidase and a T-cell origin by the presence of dot-like acid phosphatase.

Adenosine Triphosphatases↗
PubMed1980

Proliferation of T gamma cells with killer-cell activity in two patients with neutropenia and recurrent infections.

Two patients with severe granulocytopenia and recurrent infections of the skin and oropharynx had excess T lymphocytes with receptors for the Fc portion of IgG (T gamma cells) in blood and bone marrow. The abnormal T gamma cells killed antibody-sensitized target cells in vitro (killer-cell activity) but did not suppress immunoglobulin production by B lymphocytes (suppressor-cell activity). T gamma lymphocytes from normal persons showed both killer-cell activity and suppressor-cell activity. In the serum of one patient, granulocyte antibodies, possibly of an autoimmune nature, were detected. The clinical picture in conjunction with the hematologic and immunologic findings characterized the disease of both patients as a distinct entity among the chronic lymphoproliferative diseases of T-cell origin.

Aged↗
PubMed1980

Metabolic and secretory properties of peripheral and synovial granulocytes in rheumatoid arthritis.

The metabolic and secretory properties of peripheral and synovial granulocytes of patients with rheumatoid arthritis were investigated with serum- or immunoglobulin-treated zymosan as activators of cell metabolism. During isolation of the synovial cells precautions were taken to prevent in vitro phagocytosis of immune materials present in the synovial fluids. Oxygen uptake, extracellular release of lysosomal enzymes under resting and activated conditions, yield of the isolated granulocytes, and the granule enzyme content of peripheral and synovial cells did not differ significantly from those of peripheral granulocytes from healthy volunteers. In agreement with the biochemical results, intracellular inclusions could be detected in only a few synovial cells with a direct immunofluorescence technique. The possibility that formation of "ragocytes" may be an in vitro phenomenon is discussed.

Adult↗
PubMed1980

The antiperinuclear factor: II. A light microscopical and immunofluorescence study on the antigenic substrate.

The antigenic substrate of the antiperinuclear factor (APF) in human buccal mucosa cells was studied by light microscopy, cytochemistry, and the immunofluorescence technique (IFT). The cytoplasmic granules against which the APF is directed stained basophilic in light-microscopical staining techniques. The granules did not show a positive reaction product by techniques in which special chemical components are stained, and no activity of lysosomal enzymes could be identified. The use of autoantibodies and other antisera directed to distinct tissues, components, or macromolecules did not resolve the character of the antigenic granules. In addition it was shown that human vaginal epithelial cells and cryostat sections of human and rabbit buccal and oesophageal mucosa incubated with APF-positive sera showed the same fluorescent granules in the IFT as human buccal mucosa cells. Cryostat sections of rabbit buccal and oesophageal mucosa were tested as an alternative substrate in the APF test. Since the specificity as well as the sensitivity decreased when these sections were used as a substrate, they are not suitable for diagnostic purposes.

Adult↗
PubMed1979

Immune complex glomerulonephritis in chronic granulomatous disease. Case report of an eighteen-year-old girl.

An 18-year-old girl is described who developed advanced renal sclerotic lesions, probably preceded by local focal glomerulonephritis, which coexisted with a Staphylococcus aureus abscess. It is hypothesized that immune complex glomerulonephritis developed, provoked by long-term antigenic stimulation of Staphylococcus aureus. The cause of these long-term bacterial infections was a defect of the phagocytic cells to kill bacteria effectively, a disease known as chronic granulomatous disease. Therapy with intracellularly active antimicrobial agents restored the renal function till no more haemodialysis was necessary.

Adolescent↗
PubMed1978

Demonstration of non-capsulated Haemophilus influenzae in the tissues of the respiratory tract in atopic and non-atopic subjects by means of an immunofluroescence technique.

The use of an immunofluorescence method for demonstration of non-capsulated H. influenzae colonization and penetration in the tissues of the respiratory tract seems to be a useful and convenient laboratory method. However, in in the tissues of the lower respiratory tract H. influenzae in our materials could not be demonstrated.

Fluorescent Antibody Technique↗
PubMed1977

Preparation and specificity testing of a rabbit anti-human thymocyte serum (with 1 colour plate).

The preparation of a specific anti-T cell serum, applicable in the indirect immunofluorescence technique on cell suspensions, smears of peripheral blood and bone marrow and on tissue sections is described. Rabbits were immunized with thymocytes; after removal of antibodies against species-specific antigens and antigens common to leucocytes by absorptions with red cells and granulocytes, specificity for thymocytes was obtained by repeated absorptions with CLL cells, used as B cell equivalents. Subsequently, the IgG fraction of the absorbed antiserum was isolated. This antibody preparation was tested with various types of blood cells as well as with cell suspensions depleted or enriched in T cells. For the study of tissue sections it had to be absorbed with liver powder. When studying lymphatic tissue it was found to stain only thymus-dependent areas in the specimens tested.

Animals↗