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Biomedical subjects

T M Daniel

Publications and source records attributed to T M Daniel.

At least 145 records · Page 8Linked to original sources

Reactivity of purified proteins and polysaccharides from Mycobacterium tuberculosis in delayed skin test and cultured lymphocyte mitogenesis assays.

Hypersensitivity responses to two highly purified and well characterized mycobacterial proteins and a preparation of mycobacterial arabinogalactanarabinomannan were studied by using guinea pig skin tests and in vitro-cultured human lymphocyte mitogenesis stimulation assays. The purified proteins were found to elicit delayed-type hypersensitivity skin test reactions and to stimulate mitogenesis in cultured lymphocytes. The polysaccharide preparation was found to evoke delayed skin tests but not to stimulate cultured lymphocytes.

Animals↗

Purification and Characterization of Two Proteins from Culture Filtrates of Mycobacterium tuberculosis H(37)Ra Strain.

Two proteins have been purified from culture filtrates of Mycobacterium tuberculosis, H(37)Ra strain by a procedure combining gel filtration, diethylaminoethyl (DEAE)-cellulose chromatography, and zone electrophoresis. The two proteins are similar in molecular weight but differ slightly in charge. The faster migrating protein, designated a(1), is not antigenic. The slower migrating protein, designated a(2), is antigenic both with respect to antisera and as a skin-testing antigen.

Journal Article↗