Search PubMed⌕ Search

Biomedical subjects

T Lynch

Publications and source records attributed to T Lynch.

At least 127 records · Page 7Linked to original sources
PubMed1969

Growth of Physarum flavicomum and Physarum rigidum in chemically defined minimal media.

Physarum flavicomum, P. polycephalum, and P. rigidum grew at pH 4.2 in a medium composed of mineral salts, glucose, biotin, thiamine, hematin, and four amino acids. Important differences in pH tolerance were noted among the species. The minimal medium of P. flavicomum and P. polycephalum contained the amino acids methionine, glycine, and arginine, but valine was also required by P. rigidum. Starting with an inoculum of about 0.3 mg of protein per 25 ml of minimal medium, P. flavicomum and P. polycephalum grew to 23 mg and P. rigidum to 12 mg of protein per 25 ml in 3, 2, and 4 weeks, respectively. P. flavicomum and P. polycephalum grew with valine or leucine replacing arginine in the minimal medium but the growth yields and growth rates were decreased. All three species utilized homocysteine thiolactone in the minimal media in place of methionine. Serine adequately replaced glycine for P. rigidum but was inhibitory in the minimal medium of P. flavicomum or P. polycephalum unless homocysteine thiolactone also replaced methionine. Growth rates of all three organisms were increased in the presence of seven amino acids (original four plus leucine, lysine, and isoleucine).

Amino Acids↗
PubMedDate not supplied

Primary torsion dystonia due to the Tor1A GAG deletion in an Irish family.

BACKGROUND: Early, limb-onset primary torsion dystonia (PTD) is commonly due to a trinucleotide GAG deletion in the TOR1A (DYT1) gene on chromosome 9q34. The majority of carriers of this mutation conform to a characteristic phenotype that is similar in different ethnic populations. AIM: To describe the clinical features of affected members of a large Irish family with PTD due to the TOR1A deletion. METHODS: Fourteen consenting family members from three generations were examined according to a standardised protocol. RESULTS: Five affected individuals were identified. Two had a somewhat atypical phenotype with focal and segmental upper-limb dystonia without further progression. CONCLUSION: The authors describe the clinical features of PTD due to the TOR1A GAG deletion in an Irish family illustrating the presence of intrafamilial phenotypic variability.

Adult↗
PubMedDate not supplied

Substance abuse and prostitution.

A study of 200 street prostitutes documented a high prevalence of alcohol and drug abuse in their family of origin, during the drift into prostitution and as part of prostitution. Additionally, the study documented a high prevalence of substance abuse among the child molesters and rapists of the subjects. The existence of a relationship between substance abuse and prostitution in and of itself does not imply causality. It is not clear whether substance abuse is one of the factors that pushed these women into prostitution (as noted earlier, 55% of the subjects reported being addicted prior to their prostitution involvement) or whether it was prostitution that caused their drug involvement (30% became addicted following and 15% concurrently with their prostitution involvement). Most likely, both prostitution and substance abuse are the behavioral translations of these women's endless cycles of victimization and severely disturbed backgrounds, as well as an expression of the self-destructive pull, the sense of hopelessness, helplessness, negative self-concept and psychological paralysis reported by almost every subject in the study.

Adolescent↗
PubMedDate not supplied

LC/MS determination of the intracellular concentration of two novel aryl phosphoramidate prodrugs of PMPA and their metabolites in dog PBMC.

LC/MS assays were developed to determine the plasma and intracellular concentrations of two aryl phosphoramidate prodrugs of the nucleotide analog 9-[2-R-(phosphonomethoxy)propyl]adenine. LC/MS was used to demonstrate the presence of high concentrations of PMPA in peripheral blood mononucleocytes following oral administration of prodrugs in dogs. High concentrations of PMPA and active metabolite were detected in MT-2 cells incubated with prodrug using an ion-pairing LC/MS assay.

Adenine↗
PubMedDate not supplied

Changing the guard: how the Mewburn Veterans Centre received new owners.

The consolidation of programs and services is currently of great interest in Canadian health care. This article describes the process that was used to transfer a 146-bed continuing care centre from one hospital organization to another. Leadership, team work, structure and communication were found to be the keys to success in dealing with the many problems and complexities of a facility transfer.

Alberta↗
PubMedDate not supplied

Determining physicians' knowledge and attitudes when prescribing drugs to treat gastrointestinal disorders.

OBJECTIVE: Peptic ulcer disease (PUD) affects 10% to 15% of the US population. The causes of PUD are many, including high acid production, low bicarbonate secretion, and infection due to Helicobacter pylori. In 1992, the Vermont Medicaid Program noted a significant increase in prescription drug expenditures, particularly in the area of treatment of PUD. The purpose of this study was to review Medicaid prescription data and to use focus group methodology to gain an understanding of rural nonacademic and semiurban academic physicians' prescribing decisions regarding the treatment of PUD. METHODS: Pharmaceutical data from 1991 and 1992, provided by the Department of Social Welfare, Medicaid Division, was reviewed. Focus group discussions were held with primary-care providers from rural and semiurban regions with Vermont. RESULTS: Pharmaceutical review revealed that expenditures increased 21% for gastrointestinal drugs from 1991 to 1992. Drug utilization review of pharmaceutical prescriptions revealed that H2 antagonists were being prescribed for greater than the recommended 6 to 8 weeks in 60% of the cases. Focus group discussions showed that rural nonacademic and urban academic physicians had similar concerns and management plans in regard to their patients with peptic ulcer disease. However, differences existed in physician perceptions regarding pharmaceutical effectiveness of various agents for the treatment of PUD. CONCLUSIONS: Physician education outreach programs should be designed to standardize treatment methodology for PUD throughout the state. This standardization of treatment could have a significant impact on healthcare costs and the ease with which patients can eradicate this disease.

Clinical Competence↗