Thromboplastin activity of mouse peritoneal macrophages.
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Biomedical subjects
T Lyberg
Publications and source records attributed to T Lyberg.
Modulation of adhesion molecule profiles on alveolar macrophages and blood leukocytes.
BACKGROUND: Cell adhesion molecules are believed to be essential for blood cell recruitment to the lung and for the movement of alveolar macrophages (AM) within the lung. OBJECTIVE: To investigate the expression pattern of L-selectin and beta(2) integrins on blood leukocytes and AM, including AM of various maturity. METHODS: Flow cytometry was used to study the expression of L-selectin (CD62L) and of the beta(2) integrins CD11a, CD11b, and CD11c on AM (including density-defined subpopulations), monocytes (Mo), polymorphonuclear neutrophils (PMN) and lymphocytes (Ly) sampled from healthy individuals, during incubation with and without lipopolysaccharide (LPS). RESULTS: A significantly different modulation pattern of beta(2) integrins and L-selectin was demonstrated on Mo and AM, cells of the same differentiation lineage. In contrast to AM, Mo had a marked ability to respond to LPS stimulation by increased expression of CD11a, CD11b and CD11c and decreased expression of L- selectin. These molecules were expressed to a similar degree on AM, whereas the basal levels of CD11b and L-selectin were considerably higher on Mo than on AM. A significantly different expression of CD11a as well as differences in the regulation of L-selectin during incubation were also demonstrated between density-defined subpopulations of AM. CD11a could not be upregulated on PMN, otherwise the modulation patterns of CD11b, CD11c and L-selectin were similar to that on Mo. The expression of CD11a on Ly was 3- to 6-fold higher than on Mo, PMN and AM. The level of CD11b decreased significantly upon incubation (uninfluenced by LPS stimulation), and CD11c was hardly expressed on Ly. The level of L-selectin on Ly was higher than on Mo, AM and PMN and was not decreased during incubation. CONCLUSION: Developmental origin, degree of cell differentiation (maturity) as well as different environmental conditions all heavily influence the expression and modulation pattern of beta(2) integrins and L-selectin on leukocytes and Mo-derived AM.
Adult↗
Subpopulations of human lung alveolar macrophages: ultrastructural features.
Lung alveolar macrophages (LAM), obtained by bronchoalveolar lavage of healthy donors, were separated into four subfractions on discontinuous gradients of Percoll and subjected to light microscopic, transmission (TEM) and scanning electron microscopic (SEM) studies. Alveolar macrophage morphometric analysis was performed on cytocentrifuged preparations. TEM of subpopulations revealed considerable morphologic heterogeneity. By SEM, cells of the most dense (D) subfraction were small, round, and, typically, the surface was highly ruffled with small membrane pseudopods. Cells of the least dense subfraction (A) showed a low degree of membrane folding or filopodia and were often totally disorganized. In smokers, macrophages of fraction A had a greater area and perimeter compared with non-smokers, whereas the inverse relationship was observed for C and D cells. Also, the number of electron-dense inclusions and the level of acid phosphatase were higher in smokers than in non-smokers. Coupled with functional heterogeneity the morphologic differences described in this paper suggest that density-separated subpopulations of LAM may represent different stages of differentiation or maturation.
Adult↗
Cephalometric analysis and surgical treatment of patients with obstructive sleep apnea syndrome. A preliminary report.
Oropharyngeal soft tissue profiles were studied by cephalometric analysis in 25 patients with obstructive sleep apnea syndrome (OSAS) and 10 controls. The length of the soft palate was significantly longer in patients (mean 48 mm) than in controls (mean 35 mm), as was the distance of close contact between the tongue and the soft palate. The thickness of the soft palate measured in the midsagittal plane was larger (mean 14 mm) than in the control group (mean 11 mm). The hyoid bone was more inferiorly positioned in patients than in controls, apparently giving the tongue a more upright position with more of the tongue tissue at the hypopharyngeal level than found in normals. In patients, the nasopharyngeal airway space, as well as the oropharyngeal airway space, had significantly reduced anteroposterior dimensions. Based on these data a new and modified surgical technique for treatment of OSAS patients has been developed. The surgical procedure is described, and some preliminary results concerning the effect of this operation in 16 patients are reported.
Adult↗