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Biomedical subjects

T Long

Publications and source records attributed to T Long.

6 recordsLinked to original sources
PubMed1992

Recycling isoelectric focusing: use of simple buffers.

Using the recycling free-flow focusing (RF3) apparatus, we have demonstrated that single ampholytes can be utilized to establish very stable pH regions, separating all proteins into three groups: a sharply resolved zone of proteins isoelectric at the prevailing pH, this "pH window" being bracketed by zones of more acidic and/or basic proteins. The ampholytes used are either amino acids or their dipeptides and other derivatives. Where necessary, because of lack of an ampholyte with the required pH, a binary mixture of ampholytes can be utilized. The closer their isoelectric points (pI), the narrower will be the pH window, i.e., the sharper the resolution of the bracketed proteins. This method overcomes the necessity of using ill-defined commercial carrier ampholytes, such as Ampholine, for preparative isoelectric focusing. It is recommended that the ampholytes be utilized at relatively high concentration, 100mM or higher, this contributing to pH stability and minimizing protein precipitation.

Amino Acids↗
PubMed1992

'To protect the public and ensure justice is done': an examination of the Philip Donnelly case.

This paper constitutes a discussion of a case of sexual abuse of children by a nurse brought before the UKCC Professional Conduct Committee. Judgement was first deferred, and later the nurse was allowed to remain on the register and to practise as a nurse. The case is considered with reference to guidance from the UKCC in various documents. The case for removal from the register is first established, drawing on the UKCC Code of Professional Conduct and the UKCC document'... With a View to Removal From the Register...'?, and found to be more than adequate. The case for leniency is then considered with further reference to UKCC policy, but is rejected. It is concluded that, on this occasion, the Professional Conduct Committee's findings were erroneous and indefensible.

Adolescent↗
PubMed1990

The 5 and 15 minute runs as predictors of aerobic capacity in high school students.

Many coaches use field tests to estimate athlete's aerobic capacity. Recently it has been has suggested that the 5 minute run an accurate estimator of aerobic capacity as is the 15 minute aerobic run. The aim of this experiment was to determine whether the 5 minute run was as accurate an indicator of aerobic capacity as the 15 minute run. Also we wished to determine some correlational values between measured VO2max and both the 15 minute and 5 minute runs as well as determining some regression equations to calculate VO2max (mls.kg-1.min-1) given the distance run in the 5 or 15 minute runs. We found that the 15 minute run was still the best indicator of aerobic capacity for all age groups (r = 0.881, 851, 671 and 881 for 12, 13, 14, and 15 years respectively). The 5 minute run correlated significantly with the 15 minute run and with VO2max, in all cases. Correlational values were all moderate to high in nature with no correlation being lower than r = 0.534. We would conclude that the best method to estimate aerobic capacity in a field situation is to use the 15 minute run.

Adolescent↗