Frequency of lambda light chain subtypes in mouse antibodies to the 2,4-dinitrophenyl (DNP) group.
Of the three lambda chain subtypes made by inbred mice, chains of the lambda 1 subtype are much more frequent than those of the other subtypes (lambda 2,lambda 3) in antibodies (Ab) to those few antigenic structures that are known to elicit responses, in which lambda chains are the predominant type of light chain [(4-hydroxy-3-nitrophenyl)acetyl (NP) and dextran]. The reason for the frequency differences are not understood, and the large difference between the lambda 1 and lambda 3 frequencies is particularly puzzling, because in nearly all (about 95%) chains of these subtypes the N-terminal 97 or 98 amino acids are endoded by the same V lambda-gene segment. In an effort to identify an Ab response that has different lambda subtype frequencies, we analyzed the light chains of the Ab made by BALB/c and B6 mice in response to 2,4-dinitrophenylated chicken gamma globulin (DNP-CGG). We found that approximately 40% of the elicited anti-DNP molecules had lambda chains and of these approximately 40% were of the lambda 2 or lambda 3 subtype. Polyacrylamide gel electrophoresis indicated that the lambda 2 and lambda 3 chains were about equally abundant. Similar lambda subtype frequencies were found in the anti-DNP Ab produced by the hybridoma made with spleen cells from the same immunized mice. In the anti-DNP Ab elicited by DNP-CGG and in the anti-NP Ab elicited by NP-CGG the different lambda subtype frequencies (lambda 1/lambda 2 + lambda 3 = ca. 1.0-1.5 in anti-DNP and ca. 30 in anti-NP) were unaffected by immunizing mice with each of these antigens alone or with a mixture of the two. This finding, though preliminary, suggests that isotype-specific regulatory T cells are not responsible for the markedly different lambda subtype frequencies in anti-DNP and anti-NP Ab.