An examination of different mathematical models for renal function as measured by 131I-hippuran renography.
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Humans↗
Biomedical subjects
T Lindmo
Publications and source records attributed to T Lindmo.
Hyperthermia-induced shedding and masking of melanoma-associated antigen.
Hyperthermia has been reported to induce a dose-dependent reduction in the expression of melanoma-associated surface antigens. The objective of the present work was to study the mechanisms for the reduction in the expression of the p250 antigen recognized by the monoclonal antibody 9.2.27. Measurements at 37 degrees C showed that antibody binding induced a certain degree of modulation (internalization) of the melanoma-associated antigen. Masking of the antigen due to internalization and/or damage in situ, as well as shedding of the antigen, were measured after hyperthermia, and found to increase in a heat-dose-dependent manner, although for antigen masked this increase was not significant compared with control cells at 37 degrees C. The sum of antigen shed and masked after hyperthermia correlated with the overall reduction in antigen expression measured independently. During hyperthermia, antigen was shed and masked in approximately equal amounts. After the treatment, hyperthermia-induced shedding continued as a function of time and caused a further reduction in antigen expression, but masking did not differ from 37 degrees C controls.
Antibodies, Monoclonal↗
Cellular DNA content in normal human thyroid: a flow cytometric study based on frozen biopsy material.
A method is presented for preparing conventionally frozen biopsy material of thyroid tissue for flow cytometric measurement of DNA content in individual cells. The accuracy of the method was established by measuring cell suspensions of 24 biopsies of normal thyroid tissue from 20 patients. Biopsy material immersed in Histocon preservative solution prior to freezing was found to give better results than material frozen dry. All samples were found to have approximately the same modal DNA value (i.e. diploid), as determined by calibration of the fluorescence measurement with a standard of fluorescent microspheres. It was found that for an unknown sample, a deviation in modal DNA content of more than 13-14 per cent from diploid can be classified as significant by this technique.
Cell Aggregation↗