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Biomedical subjects

T L Thompson

Publications and source records attributed to T L Thompson.

107 records · Page 6Linked to original sources
PubMed1966

Lethal action of ribonuclease for thermophilic bacilli.

Thompson, T. L. (University of Nebraska, Lincoln), and J. M. Shively. Lethal action of ribonuclease for thermophilic bacilli. J. Bacteriol 91:673-676. 1966.-Exposure of a thermophilic bacillus, NU 47, to 0.2 mug/ml of pancreatic ribonuclease resulted in a drastic reduction of viable cells. The observed lethality is thought to be associated specifically with the ribonuclease, since heat treatment did not diminish activity and the addition of ribonucleic acid to the cells completely eliminated the action of the enzyme. Cells from 2-hr cultures, buffered between pH 6.0 and 7.0, were most sensitive. The lethal action was most pronounced when cells were exposed to the enzyme at temperatures between 25 and 45 C. A greater proportion of cells survived at higher temperatures. The release of ultraviolet-absorbing materials into the medium after exposure to enzyme could not be detected.

Absorption↗
PubMed1964

Isolation and preliminary characterization of bacteriophage phi-mu-4.

Shafia, Fred (University of Nebraska, Lincoln), and T. L. Thompson. Isolation and preliminary characterization of bacteriophage phimu-4. J. Bacteriol. 87:999-1002. 1964.-Bacteriophage phimu-4 was isolated from lysogenic Bacillus stearothermophilus NU strain 4, and was propagated in strain 10 of the same species. The phage was extremely host-specific. One of the 23 strains of thermophiles screened for susceptibility supported phage replication. Plaque-forming efficiency of phimu-4 depended on the agar medium employed and the temperature of incubation. Generally, media which resulted in restricted growth of the host cells on agar plates were most satisfactory for plaque formation. A latent period of 35 min was terminated in cell lysis and release of about 175 plaque-forming units (PFU) per infected cell. The phage particles, propagated in cells cultured in Trypticase (BBL)-yeast extract-dextrose-calcium chloride broth, were routinely removed from the lysate by 0.6 ammonium sulfate saturation in the cold. Phage purification was by ultracentrifugation followed by sucrose density-gradient. The phage particles appeared spherical, in electron micrographs. Particle size determination by electron micrographs indicated a diameter of less than 100 A.

Antibodies↗
PubMedDate not supplied

Specific binding of 125I-LHRH agonist to hippocampal membranes: fluctuations during the estrous cycle.

Specific binding of 125I-[D-Ala6-CH3-Leu7-Pro9,NHET]LHRH, a LHRH agonist, to hippocampal membranes prepared from ovariectomized female rates was examined. One high affinity binding site was observed with a Kd of 0.12 +/- 0.01 nM and an apparent Bmax of 13.0 +/- 3.8 fmol/mg. Luteinizing hormone-releasing hormone and the behaviorally active Ac-LHRH(5-10) were able to compete for the agonist binding site. Native LHRH had an apparent Ki of 1.73 nM, while AC-LHRH(5-10) was 30 times less potent. Competition studies examined over the rat estrous cycle revealed an eighteenfold decrease in apparent affinity during diestrus I and estrus compared with ovariectomized animals. Tissue from animals in proestrus had a Ki of 5.0 nM. Specific binding studies indicate that receptor concentration is highest in proestrus (6.11 +/- 0.90 fmol/mg) and significantly lower during estrus (2.4 +/- 0.29 fmol/mg). These data suggest that at least one fragment of native LHRH can interact with neuronal LHRH receptors and that these receptors, like those in the pituitary, can be modulated by circulating steroid hormones.

Animals↗
PubMedDate not supplied

Using successful models of care to guide the teaching of psychiatry in primary care.

The focus of efforts to improve primary care physicians' recognition and treatment of psychiatric conditions has been on enhancing the content of their education, with little attention paid to the choice of models through which the content is delivered. The authors believe that existing models of primary care delivery are analogous to Strain's six basic models of teaching psychiatry to primary care physicians. Each model is especially suited to a particular segment of the primary care physician's education; for example, the consultation model has relevance for teaching about better management of identified patients and the bridge model for enhancing recognition of unrecognized disorders. All six models have a place in both education and practice.

Alabama↗
PubMedDate not supplied

Challenges and opportunities for consultation-liaison psychiatry in the managed care environment.

While the evolving managed care revolution is dramatically changing the clinical, educational, and economic systems in which consultation-liaison (C-L) psychiatrists must operate, this revolution also present several important opportunities for those in C-L psychiatry. The authors, who are academic department chairs and formerly directed C-L divisions, discuss C-L psychiatry's potential involvement and suggest approaches to effectively address key aspects of these changes.

Forecasting↗
PubMedDate not supplied

Women's health problems in soap operas: a content analysis.

A content analysis of women's health problems in network daytime serials is reported. Results indicate a preponderance of such problems as substance abuse, falls resulting in death or injury, comas or unconsciousness, mental health concerns (most notably represented by amnesia and multiple personality), concerns about AIDS, automobile accidents, being drugged, fainting, pregnancy problems, and violent acts resulting in harm. Such concerns as breast and cervical cancer and heart disease are less frequently portrayed.

Data Collection↗
PubMedDate not supplied

Family physicians' perceptions of psychosocial disorders: survey report and educational implications.

One-hundred seventy-seven family physicians were surveyed regarding their perceptions of the types of psychosocial problems encountered in their practices, the amount of time they felt they devoted to each problem, and their interests regarding continuing psychiatric education. Patients with "multiple somatic complaints" and "symptoms of emotional distress" were reported as being encountered most frequently. Marital and family problems, alcoholism and sexual dysfunction were also ranked highly. The physicians indicated the highest interest in managing these disorders in addition to treatment of the suicidal patient. The implications of these results for psychiatric educators and liaison psychiatrists are discussed. The authors recommend using DSM-III diagnostic criteria applicable to these syndromes in formulating educational programs for primary care physicians.

Adolescent↗
PubMedDate not supplied

Effects of a psychiatric liaison program on internists' ability to assess psychosocial problems.

The ability to assess psychosocial problems of a group of primary care internal medicine residents education in a relatively unstructured psychiatric liaison program was compared to a group of internists who had no specialized psychiatric training. The primary care residents did not demonstrate a superior ability to assess patients' psychosocial problems when compared to internists without such training. The effectiveness of such unstructured liaison experiences (i.e., the Balint model of group supervision and teaching conferences) is questioned. The authors suggest that the main benefit of such informal approaches may lie in gaining initial attitudinal and administrative acceptance by nonpsychiatric physicians of liaison teaching programs. Formally structured, supervised learning experiences are probably necessary to effectively enhance internists' clinical skills and behaviors in dealing with the psychosocial and psychiatric aspects of medical care.

Colorado↗
PubMedDate not supplied

Age as a factor influencing the power and sensitivity of experiments for assessing body weight, testis size, and spermatogenesis in rats.

Data from 25 rats aged 60, 150, or 240 days (n = 75) were used to determine the number of observations per rat and or rats per treatment group needed to provide future experiments of known power and sensitivity. End points examined included body weight, testicular weight, number of resistant elongated spermatids, yields of germ cells from their younger progenitors, and germ cell:Sertoli cell ratios. Requirements differed in relation to the experimental power and sensitivity desired. However, the replication needed for detecting equivalent treatment effects on paired testes weight or the number of spermatids per gram of testis increased with age, and was twice as great for 240- vs 60-day-old rats. In contrast, approximately twice as many 60-day-old rats were required for detecting treatment effects on the number of spermatids per Sertoli cell than when 150- or 240-day-old rats were used.

Aging↗
PubMedDate not supplied

Changing relationships between testis size, Sertoli cell number and spermatogenesis in Sprague-Dawley rats.

Relationships between several reproductive characteristics were investigated in 25 Sprague-Dawley rats aged 60, 150, and 240 days (n = 75). Daily sperm production correlated with body weight (r = 0.63), paired testes weight (r = 0.68), testes weight as a percentage of body weight (r = -0.50), the number of spermatids supported per Sertoli cell (r = 0.51) and the number of Sertoli cells per gram (r = 0.89) or per testis (r = 0.95) among rats pooled across age groups. In general, the number and magnitude of significant coefficients of correlation were decreased when calculated within age groups. The latter often appeared to reflect a statistical consequence of relative homogeneity among rats rather than the absence of a biological relationship. However, the total number of Sertoli cells per testis correlated with daily sperm production within age groups, and could account for 85 to 94% of the variability in sperm production at 150 and 240 days, respectively.

Age Factors↗