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Biomedical subjects

T L Chester

Publications and source records attributed to T L Chester.

9 recordsLinked to original sources
PubMed2005

A virtual-modeling and multivariate-optimization examination of HPLC parameter interactions and opportunities for saving analysis time.

The interrelations of parameters in HPLC are very complicated, even in a simple problem. Optimization requires considering all the adjustable parameters in concert, but the amount of work required to do this experimentally is prohibitive. However, if we first choose the selectivity parameters, we can then successfully and rapidly perform a multivariate optimization of the efficiency parameters within a numerical model. By examining this process with a level of detail not normally necessary in routine work, we reveal the complexity of parameter interactions in a simple separation, and the potential for large savings of analysis time by properly balancing parameter values. We show how to reduce a 13 min experimental separation to less than 2 min without utilizing ultra-small particles or pressure beyond the capabilities of an ordinary HPLC instrument. Ultra-small particles will often improve analysis times when the separation is plate-number-limited, but if the particles are smaller than optimal for the required separation, then larger particles will require less analysis time.

Chromatography, High Pressure Liquid↗
PubMed2004

Determination of pressure-temperature coordinates of liquid-vapor critical loci by supercritical fluid flow injection analysis.

Knowledge of phase behavior as sample is transferred through a chromatograph is necessary for the user to either take advantage of desirable effects, such as peak focusing possibilities, or to avoid disastrous peak broadening. Users staying within the norms of conventional chromatographic techniques may not realize the phase behavior events that might be happening or that might be avoided by virtue of the parameter values they use. However, users working with unconventional conditions or with unconventional fluids, such as near-critical or supercritical fluids, must have an awareness of phase behavior through their chromatograph to ensure success. Complete phase diagrams of binary fluids are rare. However, most chromatographic parameters can be set using only knowledge of the temperature and pressure coordinates of the appropriate critical locus. These coordinates can be quickly determined for Type I binary mixtures using chromatographic equipment and a peak-shape observation technique to perform a simple flow injection experiment. Results and chromatographic applications of this knowledge will be summarized.

Chemical Phenomena↗
PubMed2003

Business-objective-directed, constraint-based multivariate optimization of high-performance liquid chromatography operational parameters.

The goal of a separation can be defined in terms of business needs. One goal often used is to provide the required separation in minimum time, but many other goals are also possible. These include maximizing resolution within an analysis-time limit, or minimizing the overall cost. The remaining requirements of the separation can be applied as constraints in the optimization of the goal. We will present a flexible, business-objective-based approach for optimizing the operational parameters of high performance liquid chromatography (HPLC) methods. After selecting the stationary phase and the mobile-phase components, several isocratic experiments are required to build a retention model. Multivariate optimization is performed, within the model, to find the best combination of the parameters being varied so that the result satisfies the goal to the fullest extent possible within the constraints. Interdependencies of parameters can be revealed by plotting the loci of optimal variable values or the function being optimized against a constraint. We demonstrate the concepts with a model separation originally requiring a 54 min analysis time. Multivariate optimization reduces the predicted analysis time to as short as 8 min, depending on the goals and constraints specified.

Chromatography, High Pressure Liquid↗
PubMed2001

Techniques for increasing the throughput of flow injection mass spectrometry.

Improvements to the design and operation of a Gilson 215 multiprobe liquid-handling system have resulted in a significant increase in the throughput for flow injection molecular weight characterization of combinatorial chemistry libraries. The rapid injection sequence, and subsequent increased sample throughput, is effected by directing the entire mobile-phase flow through each of the injection loops sequentially while isolating or "dead-ending" the remaining nonactive loops. This mode of operation was accomplished by incorporating column-switching valves prior to and following the set of eight parallel injectors. Analysis rates are achieved without sacrificing the integrity of the flow injection peak profile as baseline resolution is maintained for all samples. Using this system, the total analysis time for a 96-well microtiter plate has been reduced to approximately 5 min.

Journal Article↗
PubMed1995

Estimation of liquid-vapor critical loci for CO2-solvent mixtures using a peak-shape method.

Critical-mixture curves for 13 CO2-solvent binary mixtures were estimated using the peak-shape method. Mixture critical points were determined within 1 degrees C and 1 atm. The results for CO2-toluene and CO2-methanol were compared to previously reported data from high-pressure view cell studies. No more than a 3% difference was observed in the data generated by the two different techniques. A few abnormalities encountered while using the peak-shape method are also discussed.

Carbon Dioxide↗
PubMed1981

Determination of (dichloromethylene) diphosphonate in physiological fluids by ion-exchange chromatography with phosphorus-selective detection.

An analytical method is presented for the determination of (dichloromethylene) disphosphonate (Cl2MDP) in serum and urine. Cl2MDP is isolated from biological samples by adsorption onto precipitated calcium phosphate. Orthophosphate is separated from Cl2MDP by anion-exchange chromatography using AG 1-X8 resin. Detection is accomplished on-line using a flame photometric detector. Potentially interfering condensed phosphates are removed by acid hydrolysis. Sample handling losses are corrected by monitoring the recovery of a [14C] Cl2MDP spike added to the samples. Determinations of Cl2MDP to concentrations as low as 2 mumol/l are possible. Extension of the method to determine other diphosphonates is discussed.

Chromatography, Ion Exchange↗