Search PubMed⌕ Search

Biomedical subjects

T L Chambers

Publications and source records attributed to T L Chambers.

At least 55 records · Page 3Linked to original sources
PubMed1982

Oxalosis in infancy.

We describe 3 infants with nephrocalcinosis and terminal renal failure. In all 3 there was widespread oxalate deposition: biochemical evidence of primary hyperoxaluria was sought but the presence of severe renal failure and the lack of established normal values for urinary and plasma oxalate and glycollate in infants made this diagnosis difficult to establish.

Calcium Metabolism Disorders↗
PubMed1982

Modification of the AOAC gas-liquid chromatographic method for indole in shrimp: collaborative study.

Several changes were suggested for standardization of the AOAC official final action gas chromatographic method for the determination of indole in shrimp. In a collaborative study, 3 FDA laboratories compared the modified method with the current method. At a 95% confidence level, the same results were obtained for each respective sample by the AOAC or the modified method, which had the following changes. The cleanup column was standardized by drying the silica gel for 2 h at 125 degrees C and equilibrating with 3 g of water/25 g of silica gel. Concentrated ethyl acetate shrimp extracts were treated with anhydrous sodium sulfate before column cleanup and indole was eluted from the column with 15% ethyl ether/hexane. A reduced amount of the internal standard, 2-methylindole, was used to improve peak height measurements at the 25 microgram% indole level. The modified method has been adopted official first action to replace method 18.075.

Animals↗
PubMed1981

The diet composition and nutritional knowledge of patients with anorexia nervosa.

A retrospective study of typical 24-h food intake was undertaken of the diets of 17 anorexia nervosa patients during the initial and the most severe phases of their illness. Patients completed a nutritional knowledge questionnaire. Patients' diets were significantly lower in energy and in all major nutrients than those of control subjects. The proportion of energy derived from protein was significantly higher, from fats significantly lower and from carbohydrates not significantly different from that of controls. The mean intake of all nutrients in the more severe phase of illness was lower in the initial phase. Intakes of calcium, retinol activity and ascorbic acid were below RDA levels in the majority of patients, but only a few reported intakes of thiamin, riboflavin and niacin equivalent below RDA values. Most patients scored higher on the nutritional knowledge questionnaire than matched controls, particularly in respect to questions concerning caloric content of food, dieting and roughage. Not all patients obtained high nutritional knowledge scores however, and 25 per cent performed less well than selected controls.

Adolescent↗
PubMed1981

High pressure liquid chromatographic method for indole in shrimp: development of method and collaborative study.

A collaborative study on the determination of indole in shrimp was conducted in which a high pressure liquid chromatographic (HPLC) method and a spectrofluorometric method were compared with the AOAC gas-liquid chromatographic (GLC) method (18.075-18.078, 13th ed.). In the HPLC method, 10 g shrimp was blended with methanol, an internal standard was added, and the extract was filtered. Indole was separated on an octadecylsilane reverse phase column, using 60% MeOH-H2O, and quantitated with a fluorescence detector (excitation 280 nm, emission 330 nm) by comparing the indole peak height with that of an internal standard, 2-methyl-indole. Recoveries at a 25 micrograms/100 g level averaged 104% with a range of 90-127%, and at a level of 35 micrograms/100 g averaged 102% with a range of 93-112%. In the spectrofluorometric method, 25 g shrimp was extracted with 2% EtOAc-hexane. After several washes, indole was partitioned into a saturated NaCl-MeOH solution and its fluorescence was measured (excitation 280 nm, emission 332 nm). Recoveries at a 25 micrograms/100 g level averaged 93% with a range of 0-255% and at a level of 35 micrograms/100 g averaged 64% with a range of 0-107%. Recoveries obtained by the AOAC-GLC method at a level of 25 micrograms/100 g averaged 96% with a range of 81-116% and at a level of 35 micrograms/100 g averaged 101% with a range of 81-119%. The coefficients of variation were 20, 10, and 64% at a 25 micrograms/100 g level for the GLC method, the HPLC method, and the spectrofluorometric method, respectively. The HPLC method was adopted as official first action for indole levels in shrimp exceeding 1 microgram/100 g.

Animals↗
PubMed1981

Ultraviolet detection procedure for liquid chromatographic determination of indole in shrimp.

In an ultraviolet detection procedure, indole was extracted from shrimp by blending with carbonate buffer and then partitioned into ethyl acetate. Before quantitation by high pressure liquid chromatography (HPLC), interferences were removed by the use of a polyamide cleanup column. Concentrated column effluent was injected into the chromatograph. Indole was detected by monitoring absorbance at 217 nm and quantitated by comparison of indole peak height to that of an internal standard, 2-methylindole. Recoveries of indole ranged from 72 to 117%. Results agreed well with those by the official AOAC gas-liquid chromatographic (GLC) method.

Animals↗
PubMed1980

Ophthalmic manifestations of primary oxalosis.

The clinical and pathological findings of a 6-month-old infant with primary oxalosis, who died in renal failure, are presented. The oxalate crystalline deposition in the retinal pigment epithelium corresponded to the flecked retinopathy observed ophthalmoscopically. The difficulties in establishing a precise biochemical diagnosis are discussed and the relevant ophthalmic literature is reviewed.

Calcium Oxalate↗
PubMed1979

Should we follow up children with Henoch-Schönlein syndrome?

We reviewed 31 children who had had Henoch-Schönlein syndrome without nephritis at least 5 years before. None was found to have progressive renal disease and these patients do not require the same long-term follow-up as those with clinical renal involvement.

Child, Preschool↗
PubMed1978

If I were a dean.

Explore the source record for details and available documents.

College Admission Test↗
PubMed1978

Fluorometric determination of histamine in cheese.

Thirty-one samples of cheese obtained from retail outlets were analyzed for histamine, using an official AOAC fluorometric method. The types of cheese analyzed and the ranges of histamine found were: colby, 0.3--2.8; camembert, 0.4--4.2; cheddar, 1.2--5.8; gouda, 1.3--2.4; provolone, 2.0--23.5; roquefort, 1.0--16.8; mozzarella 1.6--5.0; and swiss, 0.4--250 mg histamine/100 g. Ten of the 12 samples of swiss cheese contained less than 16 mg histamine/100 g. The remaining 2 samples which contained 116 and 250 mg histamine/100 g were judged organoleptically to be of poor quality. An investigation of one processing facility showed that the production of histamine in swiss cheese may have been a result of a hydrogen peroxide/low temperature treatment of the milk supply. Recovery of histamine added to methanol extracts of cheese ranged from 93 to 105%. Histamine content was confirmed by high pressure liquid chromatographic analysis of the methanol extracts.

Cheese↗
PubMed1975

Parental attitudes to a clinical examination in paediatrics.

A selected group of parents, whose children were patients in a recent clinical examination, declared, with one exception that this experience was not disturbing to them or their child. The overall impression given was of interest in the proceedings and of satisfaction that this was a tangible method of expressing their gratitude for the care their children had received. Complaints were few and related to organization rather than to the examiners or candidates.

Anxiety↗